Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLenti6.3/V5-DEST
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00942161
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 894nts
Open reading frame: 14 to 907
CCATTGACTAACTATGGAAGATTATACCAAAATAGAGAAAATTGGAGAAGGTACCTATGG
AGTTGTGTATAAGGGTAGACACAAAACTACAGGTCAAGTGGTAGCCATGAAAAAAATCAG
ACTAGAAAGTGAAGAGGAAGGGGTTCCTAGTACTGCAATTCGGGAAATTTCTCTATTAAA
GGAACTTCGTCATCCAAATATAGTCAGTCTTCAGGATGTGCTTATGCAGGATTCCAGGTT
ATATCTCATCTTTGAGTTTCTTTCCATGGATCTGAAGAAATACTTGGATTCTATCCCTCC
TGGTCAGTACATGGATTCTTCACTTGTTAAGAGTTATTTATACCAAATCCTACAGGGGAT
TGTGTTTTGTCACTCTAGAAGAGTTCTTCACAGAGACTTAAAACCTCAAAATCTCTTGAT
TGATGACAAAGGAACAATTAAACTGGCTGATTTTGGCCTTGCCAGAGCTTTTGGAATACC
TATCAGAGTATATACACATGAGGTAGTAACACTCTGGTACAGATCTCCAGAAGTATTGCT
GGGGTCAGCTCGTTACTCAACTCCAGTTGACATTTGGAGTATAGGCACCATATTTGCTGA
ACTAGCAACTAAGAAACCACTTTTCCATGGGGATTCAGAAATTGATCAACTCTTCAGGAT
TTTCAGAGCTTTGGGCACTCCCAATAATGAAGTGTGGCCAGAAGTGGAATCTTTACAGGA
CTATAAGAATACATTTCCCAAATGGAAACCAGGAAGCCTAGCATCCCATGTCAAAAACTT
GGATGAAAATGGCTTGGATTTGCTCTCGAAAATGTTAATCTATGATCCAGCCAAACGAAT
TTCTGGCAAAATGGCACTGAATCATCCATATTTTAATGATTTGGACAATCAGATTAAGAA
GATGTAGCTTTCTGACAAAAAGTTTCCATATGTTATGTCAACAGATAGTTGTGTTTTTAT
TGTTAACTCTTGTCTATTTTTGTCTTATATATATTTCTTTGTTATCAAACTTCAGCTGTA
CTTCGTCTTCTAATTTCAAAAATATAACTTAAAAATGTAAATATTCTATATGAATTTAAA
TATAATTCTGTAAATGTGAAAAAAAAAAAAAAAAAAAAA
Protein sequence predicted based on nucleotide sequence
MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRH PNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCH SRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSAR YSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNT FPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: mammalian Marker: blasticidin
Host Type: bacterial Marker: chloramphenicol
Host Type: mammalian Marker: blasticidin
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLenti6.3/V5-DEST
Synonyms:
''
Sequencing Primer:
Forward:
CMV forward
Reverse:
V5 reverse
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection, mammalian expression expression with RSV and 5pLTR and HIV-1 psi and RRE and gp41 peptide and cPPT and CMV Promoter and CMV enhancer and attR1 and lac UV5 promoter and CmR and ccdB and attR2 and V5 and WPRE and Factor Xa site and SV40 promoter and SV40 Ori and T7 promoter and M13 forward and f1 ori and AmpR promoter and AmpR and ori and CAP binding site and lac promoter and lac operator and M13 reverse and T3 and EM7 and BlastR and 3pLTR and SV40 poly(A) signal; ampicillin resistance in bacterial, chloramphenicol resistance in bacterial, blasticidin resistance in mammalian;
Comments:
pLent6.3/V5-DEST from Invitrogen: https://www.thermofisher.com/order/catalog/product/V53306
Catalog: V53306
For research use only. Not intended for any therapeutic or diagnostic use.
The upgrade from pLenti6 to pLenti6.3 is in the addition of a WPRE cassette. Lentiviral Gateway destination vector for expression of a C-terminally V5-tagged protein.
Size (bp):
9387
Parent Vector:
None
Properties:
Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name:
Invitrogen
Publications:
None
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
V5
epitope tag from simian virus 5
4325
4366
CDS
gp41 peptide
antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)
1493
1537
CDS
CmR
confers resistance to chloramphenicol
2801
3460
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
3802
4107
CDS
Factor Xa site
Factor Xa recognition and cleavage site
4861
4872
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
7302
8162
Miscellaneous Feature
WPRE
woodchuck hepatitis virus posttranscriptional regulatory element
4390
4978
Miscellaneous Feature
cPPT
central polypurine tract and central termination sequence of HIV-1
1804
1921
Miscellaneous Feature
HIV-1 psi
packaging signal of human immunodeficiency virus type 1
457
582
Miscellaneous Feature
RRE
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
1075
1308
enhancer
CMV enhancer
human cytomegalovirus immediate early enhancer
1944
2247
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
6558
6574
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
9307
9323
promoter
lac UV5 promoter
E. coli lac promoter with an "up" mutation
2717
2747
promoter
SV40 promoter
SV40 enhancer and early promoter
4993
5309
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
6530
6548
promoter
CMV Promoter
human cytomegalovirus (CMV) immediate early promoter
2248
2451
promoter
RSV
Rous sarcoma virus enhancer/promoter
3
229
promoter
AmpR promoter
null
7197
7301
promoter
lac promoter
promoter for the E. coli lac operon
9245
9275
promoter
T3
promoter for bacteriophage T3 RNA polymerase
9344
9362
protein binding site
attR1
recombination site for the Gateway LR reaction
2568
2692
protein binding site
attR2
recombination site for the Gateway LR reaction
4148
4272
protein binding site
CAP binding site
CAP binding activates transcription in the presence of cAMP.
9209
9230
protein binding site
lac operator
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
9283
9299
rep_origin
SV40 Ori
SV40 origin of replication
5160
5295
rep_origin
f1 ori
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
6716
7171
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
8333
8921
viral LTR
5pLTR
truncated 5' long terminal repeat (LTR) from HIV-1
230
410
Gene
Gene Symbol :
CDK1
Symbol Nomenclature :
CDK1
SYNONYM :
CDC2; CDC28A; P34CDC2
Designation :
cell cycle controller CDC2|cell division control protein 2 homolog|cell division cycle 2, G1 to S and G2 to M|cell division protein kinase 1|p34 protein kinase
Full Nomenclature :
cyclin-dependent kinase 1
GENEID :
GenBank Accession :
HGNC :
MIM :
Vega :
Target GenBank:
Pathways
NCI :
AP-1 transcription factor network
NCI :
E2F transcription factor network
NCI :
FOXM1 transcription factor network
NCI :
p73 transcription factor network
NCI :
PLK1 signaling events
NCI :
Retinoic acid receptors-mediated signaling
Reactome :
Activated TLR4 signalling
Reactome :
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome :
Activation of NIMA Kinases NEK9, NEK6, NEK7
Reactome :
APC/C-mediated degradation of cell cycle proteins
Reactome :
APC/C:Cdc20 mediated degradation of Cyclin B
Reactome :
APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome :
ARMS-mediated activation
Reactome :
Axon guidance
Reactome :
Cdc20:Phospho-APC/C mediated degradation of Cyclin A
Reactome :
Cell Cycle
Reactome :
Cell Cycle Checkpoints
Reactome :
Cell Cycle, Mitotic
Reactome :
Centrosome maturation
Reactome :
Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
Reactome :
Condensation of Prometaphase Chromosomes
Reactome :
Condensation of Prophase Chromosomes
Reactome :
Cyclin A/B1 associated events during G2/M transition
Reactome :
Cyclin B2 mediated events
Reactome :
Cytokine Signaling in Immune system
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
Depolymerisation of the Nuclear Lamina
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
Downstream signal transduction
Reactome :
Downstream signaling of activated FGFR
Reactome :
E2F mediated regulation of DNA replication
Reactome :
E2F-enabled inhibition of pre-replication complex formation
Reactome :
ERK activation
Reactome :
ERK1 activation
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
FCERI mediated MAPK activation
Reactome :
Frs2-mediated activation
Reactome :
FRS2-mediated cascade
Reactome :
G0 and Early G1
Reactome :
G1/S Transition
Reactome :
G1/S-Specific Transcription
Reactome :
G2/M Checkpoints
Reactome :
G2/M DNA damage checkpoint
Reactome :
G2/M DNA replication checkpoint
Reactome :
G2/M Transition
Reactome :
Gastrin-CREB signalling pathway via PKC and MAPK
Reactome :
Golgi Cisternae Pericentriolar Stack Reorganization
Reactome :
GRB2 events in EGFR signaling
Reactome :
GRB2 events in ERBB2 signaling
Reactome :
IGF1R signaling cascade
Reactome :
Immune System
Reactome :
Innate Immune System
Reactome :
Insulin receptor signalling cascade
Reactome :
Interleukin-2 signaling
Reactome :
IRS-mediated signalling
Reactome :
IRS-related events
Reactome :
IRS-related events triggered by IGF1R
Reactome :
Loss of Nlp from mitotic centrosomes
Reactome :
Loss of proteins required for interphase microtubule organizationÿ from the centrosome
Reactome :
M Phase
Reactome :
MAP kinase activation in TLR cascade
Reactome :
MASTL Facilitates Mitotic Progression
Reactome :
Mitotic G1-G1/S phases
Reactome :
Mitotic G2-G2/M phases
Reactome :
Mitotic M-M/G1 phases
Reactome :
Mitotic Prometaphase
Reactome :
Mitotic Prophase
Reactome :
MyD88 cascade initiated on plasma membrane
Reactome :
MyD88 dependent cascade initiated on endosome
Reactome :
MyD88-independent cascade
Reactome :
MyD88:Mal cascade initiated on plasma membrane
Reactome :
NCAM signaling for neurite out-growth
Reactome :
NGF signalling via TRKA from the plasma membrane
Reactome :
Nuclear Envelope Breakdown
Reactome :
Nuclear Pore Complex (NPC) Disassembly
Reactome :
Phosphorylation of Emi1
Reactome :
Phosphorylation of proteins involved in the G2/M transition by Cyclin A:Cdc2 complexes
Reactome :
Phosphorylation of the APC/C
Reactome :
Prolonged ERK activation events
Reactome :
RAF/MAP kinase cascade
Reactome :
Recruitment of mitotic centrosome proteins and complexes
Reactome :
Recruitment of NuMA to mitotic centrosomes
Reactome :
Regulation of APC/C activators between G1/S and early anaphase
Reactome :
Regulation of mitotic cell cycle
Reactome :
Regulation of PLK1 Activity at G2/M Transition
Reactome :
Resolution of Sister Chromatid Cohesion
Reactome :
SHC-mediated signalling
Reactome :
SHC-related events
Reactome :
SHC-related events triggered by IGF1R
Reactome :
SHC1 events in EGFR signaling
Reactome :
SHC1 events in ERBB2 signaling
Reactome :
SHC1 events in ERBB4 signaling
Reactome :
Signal Transduction
Reactome :
Signaling by EGFR
Reactome :
Signaling by EGFR in Cancer
Reactome :
Signaling by ERBB2
Reactome :
Signaling by ERBB4
Reactome :
Signaling by FGFR
Reactome :
Signaling by FGFR in disease
Reactome :
Signaling by GPCR
Reactome :
Signaling by Insulin receptor
Reactome :
Signaling by Interleukins
Reactome :
Signaling by Leptin
Reactome :
Signaling by PDGF
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome :
Signalling by NGF
Reactome :
Signalling to ERKs
Reactome :
Signalling to p38 via RIT and RIN
Reactome :
Signalling to RAS
Reactome :
SOS-mediated signalling
Reactome :
Toll Like Receptor 10 (TLR10) Cascade
Reactome :
Toll Like Receptor 2 (TLR2) Cascade
Reactome :
Toll Like Receptor 3 (TLR3) Cascade
Reactome :
Toll Like Receptor 4 (TLR4) Cascade
Reactome :
Toll Like Receptor 5 (TLR5) Cascade
Reactome :
Toll Like Receptor 7/8 (TLR7/8) Cascade
Reactome :
Toll Like Receptor 9 (TLR9) Cascade
Reactome :
Toll Like Receptor TLR1:TLR2 Cascade
Reactome :
Toll Like Receptor TLR6:TLR2 Cascade
Reactome :
Toll-Like Receptors Cascades
Reactome :
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
Reactome :
TRAF6 Mediated Induction of proinflammatory cytokines
Reactome :
TRIF-mediated TLR3/TLR4 signaling
WikiPathway :
Cell cycle
WikiPathway :
DNA damage response
WikiPathway :
G1 to S cell cycle control
WikiPathway :
Integrated Cancer pathway
WikiPathway :
miRNA regulation of DNA Damage Response
WikiPathway :
TGF beta Signaling Pathway
Protein
HPRD :
00302
Clone
Cloning Information :
Organism
Species Specific ID:
Genome