Original Clone ID:
None
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLenti6.3/V5-DEST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00943974
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1440nts
Open reading frame: 1 to 1440
ATGAGCGACGTGGCTATTGTGAAGGAGGGTTGGCTGCACAAACGAGGGGAGTACATCAAG
ACCTGGCGGCCACGCTACTTCCTCCTCAAGAATGATGGCACCTTCATTGGCTACAAGGAG
CGGCCGCAGGATGTGGACCAACGTGAGGCTCCCCTCAACAACTTCTCTGTGGCGCAGTGC
CAGCTGATGAAGACGGAGCGGCCCCGGCCCAACACCTTCATCATCCGCTGCCTGCAGTGG
ACCACTGTCATCGAACGCACCTTCCATGTGGAGACTCCTGAGGAGCGGGAGGAGTGGACA
ACCGCCATCCAGACTGTGGCTGACGGCCTCAAGAAGCAGGAGGAGGAGGAGATGGACTTC
CGGTCGGGCTCACCCAGTGACAACTCAGGGGCTGAAGAGATGGAGGTGTCCCTGGCCAAG
CCCAAGCACCGCGTGACCATGAACGAGTTTGAGTACCTGAAGCTGCTGGGCAAGGGCACT
TTCGGCAAGGTGATCCTGGTGAAGGAGAAGGCCACAGGCCGCTACTACGCCATGAAGATC
CTCAAGAAGGAAGTCATCGTGGCCAAGGACGAGGTGGCCCACACACTCACCGAGAACCGC
GTCCTGCAGAACTCCAGGCACCCCTTCCTCACAGCCCTGAAGTACTCTTTCCAGACCCAC
GACCGCCTCTGCTTTGTCATGGAGTACGCCAACGGGGGCGAGCTGTTCTTCCACCTGTCC
CGGGAGCGTGTGTTCTCCGAGGACCGGGCCCGCTTCTATGGCGCTGAGATTGTGTCAGCC
CTGGACTACCTGCACTCGGAGAAGAACGTGGTGTACCGGGACCTCAAGCTGGAGAACCTC
ATGCTGGACAAGGACGGGCACATTAAGATCACAGACTTCGGGCTGTGCAAGGAGGGGATC
AAGGACGGTGCCACCATGAAGACCTTTTGCGGCACACCTGAGTACCTGGCCCCCGAGGTG
CTGGAGGACAATGACTACGGCCGTGCAGTGGACTGGTGGGGGCTGGGCGTGGTCATGTAC
GAGATGATGTGCGGTCGCCTGCCCTTCTACAACCAGGACCATGAGAAGCTTTTTGAGCTC
ATCCTCATGGAGGAGATCCGCTTCCCGCGCACGCTTGGTCCCGAGGCCAAGTCCTTGCTT
TCAGGGCTGCTCAAGAAGGACCCCAAGCAGAGGCTTGGCGGGGGCTCCGAGGACGCCAAG
GAGATCATGCAGCATCGCTTCTTTGCCGGTATCGTGTGGCAGCACGTGTACGAGAAGAAG
CTCAGCCCACCCTTCAAGCCCCAGGTCACGTCGGAGACTGACACCAGGTATTTTGATGAG
GAGTTCACGGCCCAGATGATCACCATCACACCACCTGACCAAGATGACAGCATGGAGTGT
GTGGACAGCGAGCGCAGGCCCCACTTCCCCCAGTTCTCCTACTCGGCCAGCGGCACGGCC
Protein sequence predicted based on nucleotide sequence
MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQC QLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDF RSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKI LKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLS RERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGI KDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFEL ILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKK LSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: mammalian Marker: blasticidin
Host Type: bacterial Marker: chloramphenicol
Host Type: mammalian Marker: blasticidin
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLenti6.3/V5-DEST
Synonyms:
''
Sequencing Primer:
Forward:
CMV forward
Reverse:
V5 reverse
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection, mammalian expression expression with RSV and 5pLTR and HIV-1 psi and RRE and gp41 peptide and cPPT and CMV Promoter and CMV enhancer and attR1 and lac UV5 promoter and CmR and ccdB and attR2 and V5 and WPRE and Factor Xa site and SV40 promoter and SV40 Ori and T7 promoter and M13 forward and f1 ori and AmpR promoter and AmpR and ori and CAP binding site and lac promoter and lac operator and M13 reverse and T3 and EM7 and BlastR and 3pLTR and SV40 poly(A) signal; ampicillin resistance in bacterial, chloramphenicol resistance in bacterial, blasticidin resistance in mammalian;
Comments:
pLent6.3/V5-DEST from Invitrogen: https://www.thermofisher.com/order/catalog/product/V53306
Catalog: V53306
For research use only. Not intended for any therapeutic or diagnostic use.
The upgrade from pLenti6 to pLenti6.3 is in the addition of a WPRE cassette. Lentiviral Gateway destination vector for expression of a C-terminally V5-tagged protein.
Size (bp):
9387
Parent Vector:
None
Properties:
Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name:
Invitrogen
Publications:
None
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
V5
epitope tag from simian virus 5
4325
4366
CDS
gp41 peptide
antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)
1493
1537
CDS
CmR
confers resistance to chloramphenicol
2801
3460
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
3802
4107
CDS
Factor Xa site
Factor Xa recognition and cleavage site
4861
4872
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
7302
8162
Miscellaneous Feature
WPRE
woodchuck hepatitis virus posttranscriptional regulatory element
4390
4978
Miscellaneous Feature
cPPT
central polypurine tract and central termination sequence of HIV-1
1804
1921
Miscellaneous Feature
HIV-1 psi
packaging signal of human immunodeficiency virus type 1
457
582
Miscellaneous Feature
RRE
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
1075
1308
enhancer
CMV enhancer
human cytomegalovirus immediate early enhancer
1944
2247
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
6558
6574
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
9307
9323
promoter
lac UV5 promoter
E. coli lac promoter with an "up" mutation
2717
2747
promoter
SV40 promoter
SV40 enhancer and early promoter
4993
5309
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
6530
6548
promoter
CMV Promoter
human cytomegalovirus (CMV) immediate early promoter
2248
2451
promoter
RSV
Rous sarcoma virus enhancer/promoter
3
229
promoter
AmpR promoter
null
7197
7301
promoter
lac promoter
promoter for the E. coli lac operon
9245
9275
promoter
T3
promoter for bacteriophage T3 RNA polymerase
9344
9362
protein binding site
attR1
recombination site for the Gateway LR reaction
2568
2692
protein binding site
attR2
recombination site for the Gateway LR reaction
4148
4272
protein binding site
CAP binding site
CAP binding activates transcription in the presence of cAMP.
9209
9230
protein binding site
lac operator
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
9283
9299
rep_origin
SV40 Ori
SV40 origin of replication
5160
5295
rep_origin
f1 ori
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
6716
7171
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
8333
8921
viral LTR
5pLTR
truncated 5' long terminal repeat (LTR) from HIV-1
230
410
Gene
Gene Symbol :
AKT1
Symbol Nomenclature :
AKT1
SYNONYM :
AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Designation :
PKB alpha|RAC-PK-alpha|RAC-alpha serine/threonine-protein kinase|protein kinase B alpha|proto-oncogene c-Akt|rac protein kinase alpha
Full Nomenclature :
v-akt murine thymoma viral oncogene homolog 1
GENEID :
GenBank Accession :
GenBank Accession :
HGNC :
MIM :
Vega :
Target GenBank:
Pathways
NCI :
a6b1 and a6b4 Integrin signaling
NCI :
amb2 Integrin signaling
NCI :
Angiopoietin receptor Tie2-mediated signaling
NCI :
Aurora A signaling
NCI :
BCR signaling pathway
NCI :
Caspase Cascade in Apoptosis
NCI :
CD40/CD40L signaling
NCI :
Ceramide signaling pathway
NCI :
Class I PI3K signaling events mediated by Akt
NCI :
Coregulation of Androgen receptor activity
NCI :
CXCR3-mediated signaling events
NCI :
CXCR4-mediated signaling events
NCI :
E-cadherin signaling in keratinocytes
NCI :
E-cadherin signaling in the nascent adherens junction
NCI :
Endothelins
NCI :
ErbB1 downstream signaling
NCI :
ErbB2/ErbB3 signaling events
NCI :
FAS (CD95) signaling pathway
NCI :
Fc-epsilon receptor I signaling in mast cells
NCI :
FGF signaling pathway
NCI :
FOXA2 and FOXA3 transcription factor networks
NCI :
FoxO family signaling
NCI :
Glucocorticoid receptor regulatory network
NCI :
Hedgehog signaling events mediated by Gli proteins
NCI :
HIF-1-alpha transcription factor network
NCI :
IFN-gamma pathway
NCI :
IGF1 pathway
NCI :
IL2 signaling events mediated by PI3K
NCI :
IL4-mediated signaling events
NCI :
IL6-mediated signaling events
NCI :
IL8- and CXCR1-mediated signaling events
NCI :
IL8- and CXCR2-mediated signaling events
NCI :
Insulin Pathway
NCI :
Insulin-mediated glucose transport
NCI :
Integrin-linked kinase signaling
NCI :
Integrins in angiogenesis
NCI :
LPA receptor mediated events
NCI :
mTOR signaling pathway
NCI :
Nephrin/Neph1 signaling in the kidney podocyte
NCI :
Nongenotropic Androgen signaling
NCI :
p53 pathway
NCI :
p75(NTR)-mediated signaling
NCI :
Plasma membrane estrogen receptor signaling
NCI :
Reelin signaling pathway
NCI :
Regulation of nuclear SMAD2/3 signaling
NCI :
Regulation of Telomerase
NCI :
Retinoic acid receptors-mediated signaling
NCI :
S1P3 pathway
NCI :
Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI :
Signaling events mediated by PTP1B
NCI :
Signaling events mediated by Stem cell factor receptor (c-Kit)
NCI :
Signaling events mediated by the Hedgehog family
NCI :
Signaling events mediated by VEGFR1 and VEGFR2
NCI :
TCR signaling in naïve CD4+ T cells
NCI :
TCR signaling in naïve CD8+ T cells
NCI :
Thromboxane A2 receptor signaling
NCI :
Trk receptor signaling mediated by PI3K and PLC-gamma
NCI :
VEGFR1 specific signals
NCI :
VEGFR3 signaling in lymphatic endothelium
Panther :
Angiogenesis
Panther :
Apoptosis signaling pathway
Panther :
EGF receptor signaling pathway
Panther :
Endothelin signaling pathway
Panther :
FGF signaling pathway
Panther :
Huntington disease
Panther :
Hypoxia response via HIF activation
Panther :
Inflammation mediated by chemokine and cytokine signaling pathway
Panther :
Insulin/IGF pathway-protein kinase B signaling cascade
Panther :
Interleukin signaling pathway
Panther :
p53 pathway
Panther :
p53 pathway by glucose deprivation
Panther :
p53 pathway feedback loops 2
Panther :
PI3 kinase pathway
Panther :
Ras Pathway
Panther :
T cell activation
Panther :
VEGF signaling pathway
Reactome :
Activation of BAD and translocation to mitochondria
Reactome :
Activation of BH3-only proteins
Reactome :
Adaptive Immune System
Reactome :
AKT phosphorylates targets in the cytosol
Reactome :
AKT phosphorylates targets in the nucleus
Reactome :
AKT-mediated inactivation of FOXO1A
Reactome :
Apoptosis
Reactome :
Butyrate Response Factor 1 (BRF1) destabilizes mRNA
Reactome :
CD28 co-stimulation
Reactome :
CD28 dependent PI3K/Akt signaling
Reactome :
Constitutive PI3K/AKT Signaling in Cancer
Reactome :
Costimulation by the CD28 family
Reactome :
CTLA4 inhibitory signaling
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
deactivation of the beta-catenin transactivating complex
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
Downregulation of ERBB2:ERBB3 signaling
Reactome :
Downstream signal transduction
Reactome :
Downstream signaling events of B Cell Receptor (BCR)
Reactome :
Downstream signaling of activated FGFR
Reactome :
eNOS activation
Reactome :
eNOS activation and regulation
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
G beta:gamma signalling through PI3Kgamma
Reactome :
G-protein beta:gamma signalling
Reactome :
GAB1 signalosome
Reactome :
Gene Expression
Reactome :
GPCR downstream signaling
Reactome :
GPVI-mediated activation cascade
Reactome :
Hemostasis
Reactome :
Immune System
Reactome :
Innate Immune System
Reactome :
Integrin alphaIIb beta3 signaling
Reactome :
Intrinsic Pathway for Apoptosis
Reactome :
KSRP destabilizes mRNA
Reactome :
Membrane Trafficking
Reactome :
Metabolism
Reactome :
Metabolism of mRNA
Reactome :
Metabolism of nitric oxide
Reactome :
Metabolism of RNA
Reactome :
Negative regulation of the PI3K/AKT network
Reactome :
NGF signalling via TRKA from the plasma membrane
Reactome :
PI-3K cascade
Reactome :
PI3K events in ERBB2 signaling
Reactome :
PI3K events in ERBB4 signaling
Reactome :
PI3K/AKT activation
Reactome :
PI3K/AKT Signaling in Cancer
Reactome :
PIP3 activates AKT signaling
Reactome :
Platelet activation, signaling and aggregation
Reactome :
Platelet Aggregation (Plug Formation)
Reactome :
Regulation of beta-cell development
Reactome :
Regulation of gene expression in beta cells
Reactome :
Regulation of mRNA stability by proteins that bind AU-rich elements
Reactome :
Role of LAT2/NTAL/LAB on calcium mobilization
Reactome :
Signal Transduction
Reactome :
Signaling by EGFR
Reactome :
Signaling by EGFR in Cancer
Reactome :
Signaling by ERBB2
Reactome :
Signaling by ERBB4
Reactome :
Signaling by FGFR
Reactome :
Signaling by FGFR in disease
Reactome :
Signaling by GPCR
Reactome :
Signaling by PDGF
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by the B Cell Receptor (BCR)
Reactome :
Signaling by Wnt
Reactome :
Signalling by NGF
Reactome :
TCF dependent signaling in response to WNT
Reactome :
Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
Reactome :
Translocation of GLUT4 to the plasma membrane
WikiPathway :
Alpha 6 Beta 4 signaling pathway
WikiPathway :
AMPK signaling
WikiPathway :
Androgen receptor signaling pathway
WikiPathway :
Angiogenesis
WikiPathway :
angiogenesis overview
WikiPathway :
Apoptosis
WikiPathway :
B Cell Receptor Signaling Pathway
WikiPathway :
EGF/EGFR Signaling Pathway
WikiPathway :
Endochondral Ossification
WikiPathway :
EPO Receptor Signaling
WikiPathway :
Estrogen signaling pathway
WikiPathway :
Focal Adhesion
WikiPathway :
FSH signaling pathway
WikiPathway :
IL-1 signaling pathway
WikiPathway :
IL-2 Signaling pathway
WikiPathway :
IL-3 Signaling Pathway
WikiPathway :
IL-4 signaling pathway
WikiPathway :
IL-5 signaling pathway
WikiPathway :
IL-6 signaling pathway
WikiPathway :
IL-7 signaling pathway
WikiPathway :
Insulin Signaling
WikiPathway :
Integrated Breast Cancer Pathway
WikiPathway :
Integrated Cancer pathway
WikiPathway :
Integrin-mediated cell adhesion
WikiPathway :
Kit receptor signaling pathway
WikiPathway :
Leptin signaling pathway
WikiPathway :
MAPK signaling pathway
WikiPathway :
MicroRNAs in cardiomyocyte hypertrophy
WikiPathway :
Notch Signaling Pathway
WikiPathway :
Prolactin Signaling Pathway
WikiPathway :
RANKL/RANK Signaling Pathway
WikiPathway :
Regulation of toll-like receptor signaling pathway
WikiPathway :
Signal Transduction of S1P Receptor
WikiPathway :
TCR Signaling Pathway
WikiPathway :
TFs Regulate miRNAs related to cardiac hypertrophy
WikiPathway :
TGF beta Signaling Pathway
WikiPathway :
TNF alpha Signaling Pathway
WikiPathway :
Toll-like receptor signaling pathway
WikiPathway :
TOR signaling
WikiPathway :
TSH signaling pathway
WikiPathway :
TWEAK Signaling Pathway
WikiPathway :
Wnt Signaling Pathway
Protein
HPRD :
01261
Clone
Cloning Information :
Organism
Species Specific ID:
Genome