Gene:
Gene Symbol:
DCHS2
Gene Name:
dachsous cadherin-related 2
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00945113
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 8751nts
Open reading frame: 1 to 8751
ATGAGACTGGATGAGATAACGAAATCAGAGAATGAAGACGGAGAAAAGAAGATGTTTACT
CAGCAGTTTGAAAGTACTGATGCCTGTGTATATGAGGTGTATGTGAAAATAAATTCCATT
GTGAAATCTCAGAGTCAGTGTGGATTGAAAAGAGGTAGTCCCGAGACTGAGCTCTGGGGT
GCTATAGTGTTCAGAAGCCAGGAAGAGAACGCAAAACCAGTGAAGGAAACTGAGGTGACA
GCCTCTGATGCAGATTCAGGACTCTATGGCTTTATTGAATATTCTCTTTATGATGGATTC
CTGAGCTATGAAGCACCTCAGGCATTCCGGATCGACCCTCATGATGGGCAAATCTGTGTT
TCTCAAGATATCGACAGGGAAAGGGATCCAGCTACCTATGATCTCCTGGTGGAAGCTAAG
GATGGGGATGAGCAGAGAATGACACATTTAGCCCTGGTGAAGGGTGGGCTAAGTGCCCAA
GCCTTTGTTCGTGTGGACCTGGAGGACGTGAATGATAATCATCCTGTGTTTAACCCATCA
ACCTATGTGACGAGCATCAGTGATGAGACCCAGCCAGGCACCGAGATCATCAATGTTCTT
GCCACTGACCAGGACTCTGGGATATATGGGACAGTGGCTTATGAGCTTATTCCAGGAAAC
GTGTCGTCCCTTTTTACCATTGACTCCACCACAGGACTCTATAGTCCAGAGGTAGAAATT
CTGAGTGCTGTCAACTTTTCAGCAGACAAGGAAGTAATGAACAGTTTAGAGATGTTTTTA
CCTCTGCTAAGGCACTTCAAGAAGGTTGAAAGAGATGAGGAAGCTGCAGAAAAAAAATTT
GAAGCTAGCAGAGGTTGGTTCATGAGGTTTAAGGGAAGACGCCATCTCCATTACCTAAAA
GTGCAAGATGAAGCAGCAAGTGCTGATGGAGAAGCTGCAGCAAGGTATGTAGCAAATCTA
GCTAAGATTCTTGGTGAAGGAATTATTTACTTAACATTACCTCTTAGTCATTTGGAATCT
ACCACACTTTCGTTGATGGTCTCTGCTCAAGACGGTGGTGGGCTCACAGCTGTCATTAAT
GCCGATGTCACCATACACATTTTCCAGACAACTCTGGCACCTGCTGAGTTTGAAAGGCCT
AAGTACACTTTCTTAGTTTATGAAGATGTGCCTGAAGATAGTCCCATTGGAACAGTGAAA
GCAAGAGAGCCCTTGAACTCCTCAGAACCAATCTTTTACAGGATTTCTTCTGGTGATCTC
GGCGGAAAGTTCTCCATTCACCCGCGGCTGGGCACTATTCGCACCCGGAAGCCCCTGGAT
CACGAGACGCAGCCCGTGGTTGTGCTCACGGTGCAGGCGCAGCTCGGCAGCGCCCCAGCC
TGCAGCAGCACCGAGGTCAACATAACAGTCATGGATGTCAATGACAACCACCCAGCGTTC
CTCAGGACCTCGGATGAGATTAGAATATCCCAGACCACGCCCCCTGGCACAGCCTTGTAC
CTCGCACGTGCGGAAGACAGAGACAGTGGGCGGAACGGACTCATCCGGTACTCCATCGCC
AGCCCGCAGCCAGGCGTCTTTGCCATCGACAGAGCCCTGGGGGTGCTGTTCCTCAACGGC
AGCCTGGGCGCGGGCGAGCAGCGGGAGCTCACGCTGACTCTCAGGGCCGAGGACCAAGGC
GTGCATCCTCAGGCAGCCCTGCTGGTGCTGACAGTCGTTATCGAGAAACGCGAACACAGC
CCATCCTGGACTTTCGAACATTTGGTCTATCAAGTGGAAGTCAGTGAGTCTCTCTCACCG
ATGACACAAATGCTGCAAACACAGGCGCACCCACTTGGCCCCCAGCGTGCAGCCTCGCCT
CTTAGGTACTCGCTGGAACCCAGCGTAGACTCTGCTATGTTTGGAATCCGCCCTTACACG
GGCTGGATTTATTTGCGGCGACAGTTTGACTATGAATCCACCCAAACATATAATTTTAGA
GTGTTTGCTTGGATCCCCGAGGACGGATTCTTGCAAAATGTGAGCACTACAGTCATTGTT
CGTGTCTGGGATGAGAATGACAATTCCCCCACCTTCTTGCATGATGTGTTGTTTTTGAAA
GTCGAAGAGAGCCCTGTTCCCCAAGGGGTAATAGGCAAAATTACAGCTATTGACATGGAC
TCTGGAAAGAATGGACAGCTATTATATTTCCTTTTGTCTGATGGAAAATTCTTCAAGATG
AATCCTAATACAGGTCCAGCAGGCACCATCTATGTCATAACCTGGGCAGATGGTGCTGCT
GCCTTTAGTGGGACAGACTTTGCATTCAGTTCTGATGAACTTCAAGCCTTTGTTCTCAAG
TCTCTGTTCTGTGAATTAGGAGAAGGAGAGTTAATCAATTGGGTGGCACTGGATCGTGAG
CACCGGGGGCACCATGAGATGACTGTGCTAGTGACAGACCGCGGCTCCCCACCACGAAAC
GCCACCATGGCGGTTTACGTCTCAGTTACTGACATCAATGATAACAGGCCCTTCTTCCCC
CAGTGTCTCCCTGGAAAGGAGTTACACGTGAAGGTTCTGGAAGGTCAACCAGTAAATATG
TTGGTTACAACTGTGTTTGCAAAGGATCCTGATGAAGGAAATAATGCAGAAGTTACATAC
TCAGTATCTTCAGAAGATAGTTCTGATCACTTTAAGATTGACGCCAACAATGGTGAAATA
AGAACAACCACAATACTTTCGTATGATTATAGACCTTCCTACAGAATGAGTGTCATTGCC
ACTGACCAGGGAGTGCCTCCTCTTCAAGGACAGGCAGTTGTTAATATTCAGGTGATCCCA
CTATCCAAAGGGAGAGCAATCATGTCTCAGAATATTAGACATTTAATTATACCAGAAAAT
TTGAAGCCCACAAAAATAATGAGCTTGATAAAGTCATCTGATCACCTTCAACAACATTAT
AATGGAAAGTTACATTTTAGTATTGTTGCAGATGATAAGGATGGACACTTTGAAATAGAC
AGCTCAACCGGAGACTTGTTTCTTTCTAAGGAACTTGATTATGAGACGACATCTCATTAT
CTTTTCAGAGTGATTACTACAGACCATAGCAAAAACCTTTCCCTGAGTAGCACAGTCTTC
CTTAGTATCGATGTGGAAGATCAGAATGACCATTCCCCATCTTTCCAGGATGAGCTCATT
GTGATCAGTGTAGAGGAGAATGTTCCCATAGGAACCCTGGTGTATGTCTTCAATGCCAAA
GATGATGACGGCAGTTTTTTGAACAGTAGAATACAATACTACATTGAATCCCACAACCCT
GGCACGAATCCATTTCTCATCCACCCCTCATTTGGCACACTAGTCACTGTGTCCCGTCTT
GACAGAGAAAGCATTCCAACTGTCATCCTGACAGTAACAGCATCTGATCAGGCTGTGAAT
GTGACAGACCGGCGACTGAGATCACTGACAGCACAAATAGTGATTTTGGATGTAAATGAC
CACAACCCCACTTTTATTTCTTTCCCCAATGCCCATGTCAAAGAGGATGTCACAGTGGGC
TCCTTGGTCCACCACATAACTGCTCACGATCCAGACGAAGGAAGGAATGGAAAAGTAACA
TACAGCATCCTCTCAGGAAATGAAAACATGACGTTTATGCTAGATGAGTCATCAGGCTTA
CTAACCACAACCTGTCCTTTGGATTATGAAATGAAAACTCAGCATATTCTGACTGTTCTG
GCACTGGATGATGGCACACCAGCACTTTCTTCATCCCAGACTTTGACAGTTACTGTTCTT
GATGTAAATGATGAAGCTCCAGTATTTAAGCAGCACCTGTATGAAGCCTCAGTGAAAGAA
AACCAAAATCCAGGGGAGTTTGTTACCAGGGTTGAAGCTCTGGACAGAGATTCAGGAGTC
AATTCCAAGCTTCAGTTTGAAATCATGCCAGGTGCTTCATTTGAATTATTCGAGATAAAT
TCTGACACTGGAGAGGTAGTGACAACCACCATACTTGACAGAGAAATTCAAGAAGTCTTC
ACCCTTCGAGTACTAGTACGAGATGGGGGATTCCCTTCATTGTCCAGCACCACAACAATC
CTCTGCACTGTTGAAGATGAAAACGATCACGCACCAGAGTTTATTGTTTCCAGTTATGAC
ATTGAGGTTCTGGAAAACCAGGAACCAGAGGTTGTCTATACGGTTTTAGCCTCTGATATG
GATGCTGGCAATAACAGAGCTGTTGAATATCACATAATTGATGGAAATACTGATGAGTGC
TTTACTATAAATGAGATGTCAGGAGAACTCTCAACCACTCGTGCTTTGGACCGGGAGCAA
ATCAGCAATTTTACCCTTGTCATTCTGTGCTCTGACCTGGGAGATCCACCTAGGAGCTCT
GTAATACACCTGCAAGTTAGAGTTTTGGATGCCAATGACCACAGTCCTTCTTTTCCCACA
CTTTATTACCAGTCCTCTGTGAGAGAAGATGCTGAAGTGGGAACAGTGGTTCTTGTGCTT
TCAGCTGTGGACAAGGATGAAGGCCTGAATGGGCAAACTGAGTATTTTCTGACTGATGAG
GCTTCTGGTGCATTCACCATTGATCCTATGTCAGGCACATTGAAAACCAGCAACACCCTC
GACCGTGAAGCCAGATCTCAGCATACATTTAGTGCTGTGGCCAGAGACTGTAGCATCCAG
GGTTCACGAAGCACCACTGTAATTATAAAAGTATATGTCACTGATGTTAATGACAATGAT
CCAGTTTTGGAACAGAACCCTTTTGATGTGTTTCTTTCCCCCGAGTCGCCTACAAACCAG
ACAACTGTCATTGTGAGAGCTGATGACCTGGACTTGGGGCCCAATGGAACTGTGGTTTTT
AGTTTTGCAGAGACCCAGTCAATGTTTTCTATTGATAAATACACAGGAGAAATTCAATTT
CAGCAAAATCCATCTTCAGAATACTTCCCTATCTGGCTGCAGTTAAAAGTTACAGACCAG
GGCATTCCAGCCAGGACAACCACGGGTCTCTTGGTCATTCACATGGAAGGAGAAGATGTA
AAGATTTCCTTCAGCCACCACCTGTATAAAGGGCTCGTGACTGAAAATTGTGAGGCAGGT
ACTTCTATTGTGACTGTTAAAGCTTTTGCTCCTGACTCAATTCAGGACAGCATGAAATAT
TCAATTTTTAGTGGAAATGAAGATGGAGTTCTTTCCCTGTGCTCTAAGTCAGGACAACTC
ACTGTGAAAGAACCCAAGTTTCTTGATTTTGAAGTCAGAAATGAGGTTCAACTCATCGTT
TTAGCTGAAAGTAGTGGGCATAGAGCCTATTGCAAAGTAGCAGTCTTGATACAGGATGAG
AATGATAATTCACCATGCTTTGAACAAAGCATTTACCAGGCATCAGTGTCTGAAAGCCAA
CTCTACAATGCTCATGTCATACAGGTTTTTGCTACCGACTTGGACAGTGGTTTGAACGGC
CTGATTGAGTATTCTATTCTGTCTGGCAACCAAGAAGAAGCATTCCAGATTGATGCACTG
AGTGGTGTGATAACAACAAAAGCGATTCTAGATTACGAGCTCACCAGCTCCTACAGCTTG
ATTGTCCAAGCCACAGATAAAGGGATGCCCAGGCTTTCTAATACGACTGTAATCAAGGTA
CAGGTGACTGATATAAATGACAATGCCCCAGCTTTTCTCCCCTCTGAAGCAGTGGAAATT
ACAGAAGATTCTTTGCCTGGTGTAATTGTGACTCATGTGTCAGTTCATGATGTGGATTTG
AATTCAGCTTTCATATTCAGTTTTGCCAAAGAGAGTAATCCTGGAACCAAGTTTGCTATT
GATCAGAACACTGGAGTGGTGGTGTTGGTGAAAACATTGGATTTTGAAGAAATGACTGAA
TATGAGCTGCTCATCCAAATTTCTGATTCAGTGCACTACACAGAGGGAGCACTTGTAGTC
CGTGTGCTGGATGTCAATGATAATCCACCAGTGTTTTCTCAAGATTTCTATCAGGTCACA
GTTCCTGAATCAATACCTGTGGGGTATTCAGTGCTGACTCTGTCAGCCACAGACTTAGAA
AGCAATGAGAACATTTCTTACAGAATTCTATCCTCTTCTAAGGAATTCTCCATTGATCCT
AAGAATGGCACAATATTTACTATCAGTCCCGTATTACTTCTGGATACAATATCAACAACT
CAATTTCTTGTGGAAGCCAGTGATGGTGGAAATCCTGACCTGAGAGCTCTTACTTTAGTG
GAGATAGGAATAGAAGATATGAACAATTATGCCCCTGAATTCACAGTCAAATCCTATAAT
CTTAGCCTAAGTGAGGATGCTCTGGTTGGAAGCACGCTTGTTACATTTTCAAACATCGAC
CATGACTGGACCCGTGAAAACACATATGTTGAATATTCCATCATCAGTGGTAATTCACAG
AACAATTTTCATGTGGAAACTAAGTTCTTTCATTCAGAATATCCTTATAAGCAAGTCGGT
TATCTTGTGTTGCTTCACAGTCTGGACAGAGAAGCAAGTGCTAGCCATGAGCTTGTCATT
CTGGCATCTGACAGTGGCTGCCCTCCATTGAGTTCCACAGCTGTCATATCAATACAAGTA
CTTGATGTCAATGACAATCCCCCAAACTTCAGCAGCCTGAGCTATCACACCCATGTCAAG
GAAAGCACCCCTCTAGGGAGTCACATCACTGTGGTCTCAGCAAATGACCGTGACACAGGG
TCACATGCAGAAATCATCTACAACATCATCTCTGGAAATGAGAAGGGACATTTTTACTTA
GAAGAAAACACTGGAGTTCTTTATTTGATTAAACCTCTGGATTATGAAAAAATGACAAAA
TTCACCTTAACTGTCCAAGCTTCAGATGCAGAAAAGAAACATTTTTCTTTTGCAGTTGTG
TTTGTCAGTGTCCTGGATGATAACGACCATGCACCTCAGTTTATGTTCTCAAGCTTCAGC
TGTATTGTTCCAGAAAATCTGCCTATTTCCTCTACCATATGCTCTATAAATGCTCTGGAT
TTTGATGCTGGTCCGTATGGAGAATTGACCTATTCTATTGTATCACCCTGTTTTCTCACT
CATGGAATGTCTTATGATCATGATCTCTTCCTCATTGACCCTTTGACAGGGGATATTCAT
GCTAAGCAAATCCTTGACTATGAAAATGGCAATAAATACTGCCTCACAGTCCAAGCCAAA
GACAAAGGTGATGCAACTGCCTCCTTAGTGGTCTGGGTGGATATTGAAGGGATAGATGAA
TTTGAGCCCATTTTCACTCAAGATCAGTATTTTTTCACCCTCCCAGAAAAGAATAAAGAC
AGACAGTTGATTGGCAGAGTGGAAGCCTCAGATGCAGATGCTGGTATTGATGGAGTCATT
CTTTACTCCCTTGGAACCTCATCTCCTTTCTTTTCAGTAAATAAAACCAATGGAAATATT
TATTTGATTAGAGCCCTTCCCCTAATAAAAAGTCAACTCAACAAAGAAGACACCTTGGAA
ATGAAAATAATCGCTCATAGTCCCAAATCAGATTCCAAGTTTGCATCTTGCACTGTTTTT
GTGAATGTGTCTTTCTCCTCTGAAGGAACACCCTTGGCAGTGTTCGCCAGCAGCTTTTCA
ATCAGCCTGGTGGTCTCCTTTTTAGTGTTTCTGATACTCATCTGCATTCTAATTGTAATG
ATTTTAAGACATAAACAAAAAGACACAATAAACAATTATGAGGAGAAGAAAACCTCATCT
TTAGATGCGGACTTGAGAGTGACCCGGGATGCCAGTGTGCTCAAAGCCTTCCAGAAAACT
GACGACTGCAGTAACGAGGTGGTCCCTGTGGATGCCACTCCGGAATGGTTGAGTTTAATA
AGTATCATGGAGAAGGATATTGTCAATCTGTACAGACACTCAAACTCCAGTGGCCACTGT
TCTGTGGAAGGAGAAACTGCAGAAGATAAGGAAATCCAGAGGATAAATGAGCATCCCTAC
AGAAAGTGCTCAGACTCAGCTCTGAGTGACCACGAGTCCAGGGTGCCAGACTCGGGTATC
CCGAGGGACTCAGACCAGCTCTCCTGCCTATCTGGGGAAACTGATGTGATGGTGACTGCC
GAAACAGCAGAAGCCAGCCAAACATTTGGGGAAGGAGATCAAGGGGAAGGCTGCAGCACC
ACCTGTGCTCAAAATAATGTGTTACCCCAGACAGTTCAGAAGAGAGAGGCAAAAGAGAGC
ATCCTGGCTGACGTTAGAAAAGAGTCTGTCTTTATTTCAGGTGATCAGGAAGTAAGGTGT
GCAGCTCTTTCAACTCAGACGACCTCTGATCATGATGGAAAAGACAACTATCACTGGAAT
TATCTTCTTAGTTGGGAGCCCAAATTCCAACCTCTTGCCTCAGTATTTAATGATATTGCA
AAACTAAAGGATGAACATTTGCATATGCCTGGCATTCCAAAAGAGAAGAAATCTTTTGTT
TTTCCACCCCCTTTGATAACAGCAGTAGCCCAGCCTGGGATTAAAGCAGTCCCACCAAGA
ATGCCGGCAGTAAACCTGGGGCAGGTGCCTCCGAAACACCCACGCTCTCCCATCCCCTAC
CATCTTGGTTCTCTGCCAGAAGGCATGACTCCCAATTTTTCTCCATCTCTTTCCCTATTG
ACGATGCAGCCTCCTGCCTTGTCTCCACTGTTGAGAGAAGGAGAATTATTAGGAACACAC
ATCAGTGGTACATGCCATGAACTTAAAGCAGAAGATGAAGTTCAAATATTG
Protein sequence predicted based on nucleotide sequence
MRLDEITKSENEDGEKKMFTQQFESTDACVYEVYVKINSIVKSQSQCGLKRGSPETELWG AIVFRSQEENAKPVKETEVTASDADSGLYGFIEYSLYDGFLSYEAPQAFRIDPHDGQICV SQDIDRERDPATYDLLVEAKDGDEQRMTHLALVKGGLSAQAFVRVDLEDVNDNHPVFNPS TYVTSISDETQPGTEIINVLATDQDSGIYGTVAYELIPGNVSSLFTIDSTTGLYSPEVEI LSAVNFSADKEVMNSLEMFLPLLRHFKKVERDEEAAEKKFEASRGWFMRFKGRRHLHYLK VQDEAASADGEAAARYVANLAKILGEGIIYLTLPLSHLESTTLSLMVSAQDGGGLTAVIN ADVTIHIFQTTLAPAEFERPKYTFLVYEDVPEDSPIGTVKAREPLNSSEPIFYRISSGDL GGKFSIHPRLGTIRTRKPLDHETQPVVVLTVQAQLGSAPACSSTEVNITVMDVNDNHPAF LRTSDEIRISQTTPPGTALYLARAEDRDSGRNGLIRYSIASPQPGVFAIDRALGVLFLNG SLGAGEQRELTLTLRAEDQGVHPQAALLVLTVVIEKREHSPSWTFEHLVYQVEVSESLSP MTQMLQTQAHPLGPQRAASPLRYSLEPSVDSAMFGIRPYTGWIYLRRQFDYESTQTYNFR VFAWIPEDGFLQNVSTTVIVRVWDENDNSPTFLHDVLFLKVEESPVPQGVIGKITAIDMD SGKNGQLLYFLLSDGKFFKMNPNTGPAGTIYVITWADGAAAFSGTDFAFSSDELQAFVLK SLFCELGEGELINWVALDREHRGHHEMTVLVTDRGSPPRNATMAVYVSVTDINDNRPFFP QCLPGKELHVKVLEGQPVNMLVTTVFAKDPDEGNNAEVTYSVSSEDSSDHFKIDANNGEI RTTTILSYDYRPSYRMSVIATDQGVPPLQGQAVVNIQVIPLSKGRAIMSQNIRHLIIPEN LKPTKIMSLIKSSDHLQQHYNGKLHFSIVADDKDGHFEIDSSTGDLFLSKELDYETTSHY LFRVITTDHSKNLSLSSTVFLSIDVEDQNDHSPSFQDELIVISVEENVPIGTLVYVFNAK DDDGSFLNSRIQYYIESHNPGTNPFLIHPSFGTLVTVSRLDRESIPTVILTVTASDQAVN VTDRRLRSLTAQIVILDVNDHNPTFISFPNAHVKEDVTVGSLVHHITAHDPDEGRNGKVT YSILSGNENMTFMLDESSGLLTTTCPLDYEMKTQHILTVLALDDGTPALSSSQTLTVTVL DVNDEAPVFKQHLYEASVKENQNPGEFVTRVEALDRDSGVNSKLQFEIMPGASFELFEIN SDTGEVVTTTILDREIQEVFTLRVLVRDGGFPSLSSTTTILCTVEDENDHAPEFIVSSYD IEVLENQEPEVVYTVLASDMDAGNNRAVEYHIIDGNTDECFTINEMSGELSTTRALDREQ ISNFTLVILCSDLGDPPRSSVIHLQVRVLDANDHSPSFPTLYYQSSVREDAEVGTVVLVL SAVDKDEGLNGQTEYFLTDEASGAFTIDPMSGTLKTSNTLDREARSQHTFSAVARDCSIQ GSRSTTVIIKVYVTDVNDNDPVLEQNPFDVFLSPESPTNQTTVIVRADDLDLGPNGTVVF SFAETQSMFSIDKYTGEIQFQQNPSSEYFPIWLQLKVTDQGIPARTTTGLLVIHMEGEDV KISFSHHLYKGLVTENCEAGTSIVTVKAFAPDSIQDSMKYSIFSGNEDGVLSLCSKSGQL TVKEPKFLDFEVRNEVQLIVLAESSGHRAYCKVAVLIQDENDNSPCFEQSIYQASVSESQ LYNAHVIQVFATDLDSGLNGLIEYSILSGNQEEAFQIDALSGVITTKAILDYELTSSYSL IVQATDKGMPRLSNTTVIKVQVTDINDNAPAFLPSEAVEITEDSLPGVIVTHVSVHDVDL NSAFIFSFAKESNPGTKFAIDQNTGVVVLVKTLDFEEMTEYELLIQISDSVHYTEGALVV RVLDVNDNPPVFSQDFYQVTVPESIPVGYSVLTLSATDLESNENISYRILSSSKEFSIDP KNGTIFTISPVLLLDTISTTQFLVEASDGGNPDLRALTLVEIGIEDMNNYAPEFTVKSYN LSLSEDALVGSTLVTFSNIDHDWTRENTYVEYSIISGNSQNNFHVETKFFHSEYPYKQVG YLVLLHSLDREASASHELVILASDSGCPPLSSTAVISIQVLDVNDNPPNFSSLSYHTHVK ESTPLGSHITVVSANDRDTGSHAEIIYNIISGNEKGHFYLEENTGVLYLIKPLDYEKMTK FTLTVQASDAEKKHFSFAVVFVSVLDDNDHAPQFMFSSFSCIVPENLPISSTICSINALD FDAGPYGELTYSIVSPCFLTHGMSYDHDLFLIDPLTGDIHAKQILDYENGNKYCLTVQAK DKGDATASLVVWVDIEGIDEFEPIFTQDQYFFTLPEKNKDRQLIGRVEASDADAGIDGVI LYSLGTSSPFFSVNKTNGNIYLIRALPLIKSQLNKEDTLEMKIIAHSPKSDSKFASCTVF VNVSFSSEGTPLAVFASSFSISLVVSFLVFLILICILIVMILRHKQKDTINNYEEKKTSS LDADLRVTRDASVLKAFQKTDDCSNEVVPVDATPEWLSLISIMEKDIVNLYRHSNSSGHC SVEGETAEDKEIQRINEHPYRKCSDSALSDHESRVPDSGIPRDSDQLSCLSGETDVMVTA ETAEASQTFGEGDQGEGCSTTCAQNNVLPQTVQKREAKESILADVRKESVFISGDQEVRC AALSTQTTSDHDGKDNYHWNYLLSWEPKFQPLASVFNDIAKLKDEHLHMPGIPKEKKSFV FPPPLITAVAQPGIKAVPPRMPAVNLGQVPPKHPRSPIPYHLGSLPEGMTPNFSPSLSLL TMQPPALSPLLREGELLGTHISGTCHELKAEDEVQIL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
