Gene:
Gene Symbol:
FBN3
Gene Name:
fibrillin 3
Original Clone ID:
U9662DG230-88
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00945156
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 8430nts
Open reading frame: 1 to 8430
ATGACTCTGGAGGGTCTGTATTTGGCAAGGGGCCCCCTGGCCCGGCTCCTGCTGGCCTGG
TCGGCCCTGTTGTGCATGGCAGGTGGCCAAGGCCGCTGGGACGGGGCCTTGGAGGCTGCA
GGTCCTGGACGTGTGCGGAGGCGGGGCAGCCCAGGCATCTTGCAGGGGCCGAATGTGTGC
GGCTCCCGGTTCCATGCCTACTGCTGTCCAGGCTGGAGGACATTCCCTGGCAGGAGCCAG
TGTGTCGTACCCATCTGTAGGCGCGCCTGCGGTGAAGGCTTCTGCTCCCAGCCCAACCTG
TGCACCTGTGCGGATGGGACGCTGGCTCCCAGCTGCGGGGTGAGCCGAGGGTCAGGGTGC
AGTGTGAGCTGTATGAATGGGGGCACCTGCCGGGGGGCGTCCTGTCTGTGTCAGAAGGGC
TACACAGGCACCGTGTGTGGGCAGCCCATCTGTGACCGCGGCTGCCACAATGGGGGTCGC
TGCATTGGGCCCAACCGCTGCGCCTGTGTGTATGGCTTCATGGGACCTCAATGTGAGAGA
GATTACCGGACGGGACCCTGCTTTGGCCAAGTAGGCCCCGAGGGGTGCCAGCATCAGCTG
ACGGGCCTCGTGTGCACCAAGGCACTTTGCTGTGCCACTGTGGGCCGTGCCTGGGGCCTT
CCATGTGAACTTTGCCCTGCACAGCCACACCCCTGCCGCCGCGGCTTCATCCCCAATATC
CACACGGGGGCCTGCCAAGATGTGGATGAGTGCCAGGCTGTGCCAGGCCTGTGCCAGGGA
GGCAGCTGCGTCAACATGGTGGGCTCCTTCCATTGCCGCTGTCCAGTTGGACACCGGCTC
AGTGACAGCAGCGCCGCATGTGAAGACTACCGGGCCGGCGCCTGCTTCTCAGTGCTTTTC
GGGGGCCGCTGTGCTGGAGACCTCGCCGGCCACTACACTCGCAGGCAGTGCTGCTGTGAC
AGGGGCAGGTGCTGGGCAGCTGGCCCGGTCCCTGAGCTGTGTCCTCCTCGGGGCTCCAAT
GAATTCCAGCAACTGTGCGCCCAGCGGCTGCCGCTGCTACCCGGCCACCCTGGCCTCTTC
CCTGGCCTCCTGGGCTTCGGATCCAATGGCATGGGTCCCCCTCTTGGGCCAGCGCGACTC
AACCCCCATGGCTCTGATGCGCGTGGGATCCCCAGCCTGGGCCCTGGCAACTCTAATATT
GGCACTGCTACCCTGAACCAGACCATTGACATCTGCCGACACTTCACCAACCTGTGTCTG
AATGGCCGCTGCCTGCCCACGCCTTCCAGCTACCGCTGCGAGTGTAACGTGGGCTACACC
CAGGACGTGCGCGGCGAGTGCATTGATGTAGACGAATGCACCAGCAGCCCCTGCCACCAC
GGTGACTGCGTCAACATCCCCGGCACCTACCACTGCCGGTGCTACCCGGGCTTCCAGGCC
ACGCCCACCAGGCAGGCATGCGTGGATGTGGACGAGTGCATTGTCAGTGGTGGCCTTTGT
CACCTGGGCCGCTGTGTCAACACAGAGGGCAGCTTCCAGTGTGTCTGCAATGCAGGCTTC
GAGCTCAGCCCTGACGGCAAGAACTGTGTGGACCACAACGAGTGTGCCACCAGCACCATG
TGCGTCAACGGCGTGTGTCTCAACGAGGATGGCAGCTTCTCCTGCCTCTGCAAACCCGGC
TTCCTGCTGGCGCCTGGCGGCCACTACTGCATGGACATTGACGAGTGCCAGACGCCCGGC
ATCTGCGTGAACGGCCACTGTACCAACACCGAGGGCTCCTTCCGCTGCCAGTGCCTGGGG
GGGCTGGCGGTAGGCACGGATGGCCGCGTGTGCGTGGACACCCACGTGCGCAGCACCTGC
TATGGGGCCATCGAGAAGGGCTCCTGTGCCCGCCCCTTCCCTGGCACTGTCACCAAGTCC
GAGTGCTGCTGTGCCAATCCGGACCACGGTTTTGGGGAGCCCTGCCAGCTTTGTCCTGCC
AAAGACTCCGCTGAGTTCCAGGCACTGTGCAGCAGTGGGCTTGGCATTACCACGGATGGT
CGAGACATCAACGAGTGTGCTCTGGATCCTGAGGTTTGTGCCAATGGCGTGTGCGAGAAC
CTTCGGGGCAGCTACCGCTGTGTCTGCAACCTGGGTTATGAGGCAGGTGCCTCAGGCAAG
GACTGCACAGACGTGGATGAGTGTGCCCTCAACAGCCTCCTGTGTGACAACGGGTGGTGC
CAGAATAGCCCTGGCAGCTACAGCTGCTCCTGCCCCCCCGGCTTCCACTTCTGGCAGGAC
ACGGAGATCTGCAAAGATGTCGACGAATGCCTGTCCAGCCCGTGTGTGAGTGGCGTCTGT
CGGAACCTGGCCGGCTCCTACACCTGCAAATGTGGCCCTGGCAGCCGGCTGGACCCCTCT
GGTACCTTCTGTCTAGACAGCACCAAGGGCACCTGCTGGCTGAAGATCCAGGAGAGCCGC
TGTGAGGTGAACCTTCAGGGAGCCAGCCTGCGGTCTGAGTGCTGCGCCACCCTCGGGGCA
GCCTGGGGGAGCCCCTGCGAACGCTGCGAGATCGACCCTGCCTGTGCCCGGGGCTTTGCC
CGGATGACGGGTGTCACCTGCGATGATGTGAACGAGTGTGAGTCCTTCCCGGGAGTCTGT
CCCAACGGGCGTTGCGTCAACACTGCTGGGTCTTTCCGCTGTGAGTGTCCAGAGGGCCTG
ATGCTGGACGCCTCAGGCCGGCTGTGCGTGGATGTGAGATTGGAACCATGTTTCCTGCGA
TGGGATGAGGATGAGTGTGGGGTCACCCTGCCTGGCAAGTACCGGATGGACGTCTGCTGC
TGCTCCATCGGGGCCGTGTGGGGAGTCGAGTGCGAGGCCTGCCCGGATCCCGAGTCTCTG
GAGTTCGCCAGCCTGTGCCCGCGGGGGCTGGGCTTCGCCAGCCGGGACTTCCTGTCTGGC
CGACCATTCTATAAAGATGTGAATGAATGCAAGGTGTTCCCTGGCCTCTGCACGCACGGT
ACCTGCAGAAACACGGTGGGCAGCTTCCACTGCGCCTGTGCGGGGGGCTTCGCCCTGGAT
GCCCAGGAACGGAACTGCACAGATATCGACGAGTGTCGCATCTCTCCTGACCTCTGCGGC
CAGGGCACCTGTGTCAACACGCCGGGCAGCTTTGAGTGCGAGTGTTTTCCCGGCTACGAG
AGTGGCTTCATGCTGATGAAGAACTGCATGGACGTGGACGAGTGTGCAAGGGACCCGCTG
CTCTGCCGGGGAGGCACTTGCACCAACACGGATGGGAGCTACAAGTGCCAGTGTCCCCCT
GGGCATGAGCTGACGGCCAAGGGCACTGCCTGTGAGGACATCGATGAGTGCTCCCTGAGT
GATGGCCTGTGTCCCCATGGCCAGTGTGTCAATGTCATCGGTGCCTTCCAGTGCTCCTGC
CATGCCGGCTTCCAGAGCACACCTGACCGCCAGGGCTGCGTGGACATCAACGAATGCCGG
GTCCAGAATGGTGGGTGTGACGTGCACTGTATTAACACTGAGGGCAGCTACCGGTGCAGC
TGTGGGCAGGGCTACTCGCTGATGCCCGACGGAAGGGCATGTGCAGACGTGGACGAGTGT
GAAGAGAACCCCCGCGTTTGTGACCAAGGCCACTGCACCAACATGCCAGGGGGTCACCGC
TGCCTGTGCTATGATGGCTTCATGGCCACGCCAGACATGAGGACATGTGTTGATGTGGAT
GAGTGTGACCTGAACCCTCACATCTGCCTCCATGGGGACTGCGAGAACACGAAGGGTTCC
TTTGTCTGCCACTGTCAGCTGGGCTACATGGTCAGGAAGGGGGCCACAGGCTGCTCTGAT
GTGGATGAATGCGAGGTTGGAGGACACAACTGTGACAGTCACGCCTCCTGTCTCAACATC
CCGGGGAGTTTCAGCTGTAGGTGCCTGCCAGGCTGGGTGGGGGATGGCTTCGAATGTCAC
GACCTGGATGAATGCGTCTCCCAGGAGCACCGGTGCAGCCCAAGAGGTGACTGTCTCAAT
GTCCCTGGCTCCTACCGCTGCACCTGCCGCCAGGGCTTTGCCGGGGATGGCTTCTTCTGC
GAAGACAGGGATGAATGTGCCGAGAACGTGGACCTCTGTGACAACGGGCAGTGCCTCAAT
GCGCCCGGCGGGTACCGCTGTGAATGTGAGATGGGCTTTGACCCCACCGAGGACCACCGG
GCCTGCCAGGATGTGGACGAGTGTGCGCAAGGGAACCTCTGTGCATTTGGGAGCTGTGAG
AACCTGCCTGGAATGTTCCGCTGCATCTGCAATGGTGGCTACGAACTGGACCGAGGGGGT
GGCAACTGCACAGACATCAACGAGTGTGCAGACCCAGTAAACTGCATCAACGGCGTGTGC
ATTAACACCCCCGGCAGCTACCTCTGCAGCTGCCCCCAGGATTTTGAGCTGAACCCCAGC
GGAGTGGGCTGCGTGGACACTCGGGCCGGGAACTGTTTCCTGGAGACGCATGACCGAGGG
GACAGTGGCATTTCCTGCAGTGCCGAGATCGGAGTTGGTGTCACCCGAGCTTCCTGCTGT
TGCTCCCTGGGCCGGGCTTGGGGCAATCCCTGTGAGCTGTGCCCTATGGCCAACACCACT
GAGTACAGAACCCTGTGCCCGGGTGGTGAGGGCTTCCAGCCTAACCGCATCACTGTCATT
CTGGAAGACATCGACGAGTGCCAAGAGCTGCCAGGGCTGTGTCAGGGGGGTGACTGCGTC
AACACGTTTGGCAGTTTCCAGTGTGAGTGCCCACCTGGCTACCACCTCAGTGAGCACACC
CGCATCTGTGAGGATATTGACGAATGCTCCACACACTCCGGCATCTGTGGCCCTGGCACC
TGCTACAACACCCTGGGGAACTACACCTGTGTCTGCCCTGCAGAGTACCTCCAAGTCAAT
GGTGGCAACAACTGCATGGATATGAGGAAGAGTGTCTGCTTCCGGCACTATAACGGCACA
TGTCAAAATGAGCTGGCCTTCAACGTGACCCGGAAAATGTGTTGCTGCTCCTACAACATT
GGCCAGGCCTGGAATAGACCCTGTGAGGCCTGCCCCACTCCCATCAGTCCTGACTACCAG
ATCCTGTGTGGAAATCAGGCCCCGGGATTCCTCACTGACATCCACACGGGGAAGCCCCTT
GACATTGATGAGTGTGGGGAGATCCCCGCCATCTGTGCCAATGGCATCTGCATAAACCAG
ATCGGGAGTTTCCGCTGCGAGTGCCCCGCAGGCTTCAACTACAACAGCATCCTGCTGGCT
TGTGAAGATGTCGATGAGTGTGGCAGCAGGGAGAGTCCCTGCCAGCAGAATGCTGACTGC
ATCAACATCCCCGGTAGCTACCGCTGCAAGTGCACCCGAGGGTACAAACTGTCGCCAGGC
GGGGCTTGTGTGGGACGGAATGAGTGTCGGGAGATCCCGAATGTCTGTAGCCATGGTGAC
TGCATGGACACAGAAGGCAGCTACATGTGTCTGTGTCACCGTGGATTCCAGGCCTCTGCA
GACCAGACCCTGTGCATGGACATTGACGAGTGTGACCGGCAGCCTTGTGGAAATGGGACC
TGCAAGAACATCATTGGCTCCTACAACTGCCTCTGCTTCCCTGGCTTTGTGGTGACACAC
AATGGGGATTGTGTGGATTTTGATGAGTGTACTACCCTGGTGGGGCAGGTGTGCCGATTT
GGCCATTGCCTCAACACAGCTGGTTCCTTCCACTGCCTCTGCCAGGATGGCTTTGAGCTC
ACAGCTGATGGGAAGAACTGTGTGGACACCAATGAGTGCCTCAGCCTTGCAGGAACCTGC
CTACCCGGCACTTGCCAGAACCTCGAGGGCTCCTTCCGCTGCATCTGTCCCCCTGGCTTC
CAGGTGCAGAGTGACCACTGCATTGATATCGACGAGTGCTCAGAGGAGCCCAACCTCTGC
CTCTTTGGCACCTGTACCAACAGCCCTGGGAGCTTCCAGTGCCTCTGCCCACCTGGCTTT
GTCCTCTCTGACAATGGGCACCGTTGCTTTGACACACGGCAGAGTTTCTGCTTCACCCGT
TTTGAGGCTGGGAAGTGCTCGGTGCCCAAAGCTTTCAACACCACCAAGACCCGCTGCTGC
TGCAGTAAGAGGCCTGGGGAGGGCTGGGGAGACCCCTGCGAACTGTGTCCCCAGGAGGGC
AGCGCTGCCTTTCAGGAGCTCTGCCCCTTTGGCCACGGGGCAGTCCCAGGCCCGGATGAC
TCCCGAGAAGACGTGAATGAGTGTGCAGAGAACCCTGGCGTCTGCACTAACGGCGTCTGT
GTCAACACCGATGGATCCTTCCGCTGTGAGTGTCCCTTTGGCTACAGCCTGGACTTCACT
GGCATCAACTGTGTGGACACAGACGAGTGCTCTGTCGGCCACCCCTGTGGGCAAGGGACA
TGCACCAATGTCATCGGAGGCTTCGAATGTGCCTGTGCTGACGGCTTTGAGCCTGGCCTC
ATGATGACCTGCGAGGACATCGACGAATGCTCCCTGAACCCGCTGCTCTGTGCCTTCCGC
TGCCACAATACCGAGGGCTCCTACCTGTGCACCTGTCCAGCCGGCTACACCCTGCGGGAG
GATGGGGCCATGTGTCGAGATGTGGACGAGTGTGCAGATGGTCAGCAGGACTGCCACGCC
CGGGGCATGGAGTGCAAGAACCTCATCGGTACCTTCGCGTGCGTCTGTCCCCCAGGCATG
CGGCCCCTGCCTGGCTCTGGGGAGGGCTGCACAGATGACAATGAATGCCACGCTCAGCCT
GACCTCTGTGTCAACGGCCGCTGTGTCAACACCGCGGGCAGCTTCCGGTGCGACTGTGAT
GAGGGATTCCAGCCCAGCCCCACCCTTACCGAGTGCCACGACATCCGGCAGGGGCCCTGC
TTTGCCGAGGTGCTGCAGACCATGTGCCGGTCTCTGTCCAGCAGCAGTGAGGCTGTCACC
AGGGCCGAGTGCTGCTGTGGGGGTGGCCGGGGCTGGGGGCCCCGCTGCGAGCTCTGTCCC
CTGCCCGGCACCTCTGCCTACAGGAAGCTGTGCCCCCATGGCTCAGGCTACACTGCTGAG
GGCCGAGATGTAGATGAATGCCGTATGCTTGCTCACCTGTGTGCTCATGGGGAGTGCATC
AACAGCCTTGGCTCCTTCCGCTGCCACTGTCAGGCCGGGTACACACCGGATGCTACTGCT
ACTACCTGCCTGGATATGGATGAGTGCAGCCAGGTCCCCAAGCCATGTACCTTCCTCTGC
AAAAACACGAAGGGCAGTTTCCTGTGCAGCTGTCCCCGAGGCTACCTGCTGGAGGAGGAT
GGCAGGACCTGCAAAGACCTGGACGAATGCACCTCCCGGCAGCACAACTGTCAGTTCCTC
TGTGTCAACACTGTGGGCGCCTTCACCTGCCGCTGTCCGCCCGGCTTCACCCAGCACCAC
CAGGCCTGCTTCGACAATGATGAGTGCTCAGCCCAGCCTGGCCCATGTGGTGCCCACGGG
CACTGCCACAACACCCCGGGCAGCTTCCGCTGTGAATGCCACCAAGGCTTCACCCTGGTC
AGCTCAGGCCATGGCTGTGAAGATGTGAATGAATGTGATGGGCCCCACCGCTGCCAGCAT
GGCTGTCAGAACCAGCTAGGGGGCTACCGCTGCAGCTGCCCCCAGGGTTTCACCCAGCAC
TCCCAGTGGGCCCAGTGTGTGGATGAGAATGAGTGTGCCCTGTCGCCCCCCACCTGCGGG
AGCGCCTCCTGTCGCAACACTCTTGGTGGCTTCCGCTGCGTCTGCCCCTCTGGCTTTGAC
TTTGATCAGGCCCTCGGGGGCTGCCAGGAGGTGGATGAGTGCGCCGGACGGCGTGGCCCC
TGTAGCTACAGCTGTGCCAACACGCCTGGTGGCTTCCTGTGCGGCTGTCCTCAAGGCTAC
TTCCGGGCTGGGCAAGGGCACTGTGTCTCCGGCCTGGGCTTCAGCCCCGGACCCCAGGAC
ACCCCGGACAAAGAGGAGCTGCTCTCGTCTGAAGCCTGCTACGAATGCAAGATCAATGGC
CTCTCCCCTCGGGACCGGCCACGACGCAGTGCCCACAGGGACCACCAGGTGAACCTGGCC
ACCCTTGACTCCGAGGCCCTGCTGACCTTGGGCCTGAACCTCTCACACCTGGGCCGGGCC
GAGCGCATCCTGGAGCTCCGGCCGGCCCTGGAGGGTCTAGAGGGCCGGATCCGCTACGTC
ATCGTCCGCGGAAACGAGCAAGGTTTCTTTCGCATGCATCACCTCCGTGGCGTCAGCTCC
CTGCAGCTGGGGCGGAGGCGGCCGGGGCCTGGAACCTACCGGCTGGAGGTGGTGAGCCAC
ATGGCAGGACCCTGGGGTGTCCAGCCAGAGGGGCAGCCAGGGCCATGGGGCCAGGCCTTG
AGGCTGAAGGTGCAGCTGCAGTTGCTTTTG
Protein sequence predicted based on nucleotide sequence
MTLEGLYLARGPLARLLLAWSALLCMAGGQGRWDGALEAAGPGRVRRRGSPGILQGPNVC GSRFHAYCCPGWRTFPGRSQCVVPICRRACGEGFCSQPNLCTCADGTLAPSCGVSRGSGC SVSCMNGGTCRGASCLCQKGYTGTVCGQPICDRGCHNGGRCIGPNRCACVYGFMGPQCER DYRTGPCFGQVGPEGCQHQLTGLVCTKALCCATVGRAWGLPCELCPAQPHPCRRGFIPNI HTGACQDVDECQAVPGLCQGGSCVNMVGSFHCRCPVGHRLSDSSAACEDYRAGACFSVLF GGRCAGDLAGHYTRRQCCCDRGRCWAAGPVPELCPPRGSNEFQQLCAQRLPLLPGHPGLF PGLLGFGSNGMGPPLGPARLNPHGSDARGIPSLGPGNSNIGTATLNQTIDICRHFTNLCL NGRCLPTPSSYRCECNVGYTQDVRGECIDVDECTSSPCHHGDCVNIPGTYHCRCYPGFQA TPTRQACVDVDECIVSGGLCHLGRCVNTEGSFQCVCNAGFELSPDGKNCVDHNECATSTM CVNGVCLNEDGSFSCLCKPGFLLAPGGHYCMDIDECQTPGICVNGHCTNTEGSFRCQCLG GLAVGTDGRVCVDTHVRSTCYGAIEKGSCARPFPGTVTKSECCCANPDHGFGEPCQLCPA KDSAEFQALCSSGLGITTDGRDINECALDPEVCANGVCENLRGSYRCVCNLGYEAGASGK DCTDVDECALNSLLCDNGWCQNSPGSYSCSCPPGFHFWQDTEICKDVDECLSSPCVSGVC RNLAGSYTCKCGPGSRLDPSGTFCLDSTKGTCWLKIQESRCEVNLQGASLRSECCATLGA AWGSPCERCEIDPACARGFARMTGVTCDDVNECESFPGVCPNGRCVNTAGSFRCECPEGL MLDASGRLCVDVRLEPCFLRWDEDECGVTLPGKYRMDVCCCSIGAVWGVECEACPDPESL EFASLCPRGLGFASRDFLSGRPFYKDVNECKVFPGLCTHGTCRNTVGSFHCACAGGFALD AQERNCTDIDECRISPDLCGQGTCVNTPGSFECECFPGYESGFMLMKNCMDVDECARDPL LCRGGTCTNTDGSYKCQCPPGHELTAKGTACEDIDECSLSDGLCPHGQCVNVIGAFQCSC HAGFQSTPDRQGCVDINECRVQNGGCDVHCINTEGSYRCSCGQGYSLMPDGRACADVDEC EENPRVCDQGHCTNMPGGHRCLCYDGFMATPDMRTCVDVDECDLNPHICLHGDCENTKGS FVCHCQLGYMVRKGATGCSDVDECEVGGHNCDSHASCLNIPGSFSCRCLPGWVGDGFECH DLDECVSQEHRCSPRGDCLNVPGSYRCTCRQGFAGDGFFCEDRDECAENVDLCDNGQCLN APGGYRCECEMGFDPTEDHRACQDVDECAQGNLCAFGSCENLPGMFRCICNGGYELDRGG GNCTDINECADPVNCINGVCINTPGSYLCSCPQDFELNPSGVGCVDTRAGNCFLETHDRG DSGISCSAEIGVGVTRASCCCSLGRAWGNPCELCPMANTTEYRTLCPGGEGFQPNRITVI LEDIDECQELPGLCQGGDCVNTFGSFQCECPPGYHLSEHTRICEDIDECSTHSGICGPGT CYNTLGNYTCVCPAEYLQVNGGNNCMDMRKSVCFRHYNGTCQNELAFNVTRKMCCCSYNI GQAWNRPCEACPTPISPDYQILCGNQAPGFLTDIHTGKPLDIDECGEIPAICANGICINQ IGSFRCECPAGFNYNSILLACEDVDECGSRESPCQQNADCINIPGSYRCKCTRGYKLSPG GACVGRNECREIPNVCSHGDCMDTEGSYMCLCHRGFQASADQTLCMDIDECDRQPCGNGT CKNIIGSYNCLCFPGFVVTHNGDCVDFDECTTLVGQVCRFGHCLNTAGSFHCLCQDGFEL TADGKNCVDTNECLSLAGTCLPGTCQNLEGSFRCICPPGFQVQSDHCIDIDECSEEPNLC LFGTCTNSPGSFQCLCPPGFVLSDNGHRCFDTRQSFCFTRFEAGKCSVPKAFNTTKTRCC CSKRPGEGWGDPCELCPQEGSAAFQELCPFGHGAVPGPDDSREDVNECAENPGVCTNGVC VNTDGSFRCECPFGYSLDFTGINCVDTDECSVGHPCGQGTCTNVIGGFECACADGFEPGL MMTCEDIDECSLNPLLCAFRCHNTEGSYLCTCPAGYTLREDGAMCRDVDECADGQQDCHA RGMECKNLIGTFACVCPPGMRPLPGSGEGCTDDNECHAQPDLCVNGRCVNTAGSFRCDCD EGFQPSPTLTECHDIRQGPCFAEVLQTMCRSLSSSSEAVTRAECCCGGGRGWGPRCELCP LPGTSAYRKLCPHGSGYTAEGRDVDECRMLAHLCAHGECINSLGSFRCHCQAGYTPDATA TTCLDMDECSQVPKPCTFLCKNTKGSFLCSCPRGYLLEEDGRTCKDLDECTSRQHNCQFL CVNTVGAFTCRCPPGFTQHHQACFDNDECSAQPGPCGAHGHCHNTPGSFRCECHQGFTLV SSGHGCEDVNECDGPHRCQHGCQNQLGGYRCSCPQGFTQHSQWAQCVDENECALSPPTCG SASCRNTLGGFRCVCPSGFDFDQALGGCQEVDECAGRRGPCSYSCANTPGGFLCGCPQGY FRAGQGHCVSGLGFSPGPQDTPDKEELLSSEACYECKINGLSPRDRPRRSAHRDHQVNLA TLDSEALLTLGLNLSHLGRAERILELRPALEGLEGRIRYVIVRGNEQGFFRMHHLRGVSS LQLGRRRPGPGTYRLEVVSHMAGPWGVQPEGQPGPWGQALRLKVQLQLLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
