Gene:
Gene Symbol:
ZFPM1
Gene Name:
zinc finger protein, FOG family member 1
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00945216
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3021nts
Open reading frame: 1 to 3021
ATGTCCAGGCGGAAACAGAGCAACCCCCGGCAGATCAAGCGTTCCCTCGGAGACATGGAG
GCCAGAGAGGAGGTGCAGTTGGTGGGTGCCAGCCACATGGAGCAAAAGGCCACGGCACCT
GAAGCCCCGAGCCCTCCCAGCGCAGATGTTAACTCACCCCCACCGCTGCCGCCCCCCACA
TCCCCAGGAGGCCCCAAGGAGCTGGAAGGACAGGAACCAGAACCCAGGCCCACGGAGGAA
GAGCCGGGCAGTCCCTGGAGCGGGCCAGACGAGCTGGAGCCGGTGGTGCAGGATGGGCAG
AGGCGCATACGGGCCCGACTCAGCCTCGCCACGGGCCTGTCCTGGGGCCCGTTCCATGGG
AGTGTCCAGACCAGAGCCTCATCCCCCAGGCAGGCGGAGCCGAGCCCAGCCCTGACCCTG
CTGCTGGTGGACGAGGCCTGCTGGCTGAGGACGCTGCCCCAGGCCCTGACTGAGGCCGAG
GCCAACACAGAGATCCACAGGAAGGATGACGCACTCTGGTGCAGGGTCACCAAGCCGGTG
CCTGCGGGGGGACTCCTGAGCGTGCTCCTCACGGCCGAGCCCCACAGCACCCCCGGCCAC
CCTGTGAAGAAGGAGCCAGCAGAGCCCACGTGCCCGGCCCCTGCACACGACCTCCAGCTC
CTGCCCCAGCAGGCCGGGATGGCCTCCATCCTTGCCACCGCAGTGATCAACAAAGACGTC
TTCCCCTGCAAGGACTGTGGCATCTGGTACCGCAGCGAGCGCAACCTGCAGGCGCACCTG
CTCTACTACTGCGCCAGCCGCCAGGGCACCGGCTCCCCGGCCGCAGCCGCCACAGACGAG
AAGCCCAAAGAGACCTACCCCAACGAGCGCGTCTGCCCCTTCCCCCAGTGCCGCAAGAGC
TGCCCCAGCGCCAGCTCCCTGGAGATCCACATGCGCAGCCACAGCGGAGAGCGGCCCTTC
GTGTGCCTGATCTGCCTGTCGGCCTTCACCACCAAGGCCAACTGCGAGCGGCACCTCAAG
GTGCACACGGACACGCTGAGCGGTGTCTGCCACAGCTGTGGCTTCATCTCCACCACAAGG
GACATCCTCTACAGCCACTTGGTCACCAACCACATGGTCTGCCAGCCTGGCTCCAAGGGT
GAGATCTACTCGCCAGGGGCCGGACACCCAGCAACCAAGCTGCCCCCAGACAGTCTGGGC
AGCTTCCAGCAGCAGCACACGGCCCTGCAAGGCCCCCTGGCCTCCGCGGACCTGGGCCTG
GCGCCCACCCCATCGCCAGGACTGGACAGAAAGGCCCTGGCCGAGGCCACCAACGGAGAG
GCCAGAGCGGAGCCTCTGGCCCAGAATGGAGGCAGCAGCGAGCCCCCGGCGGCCCCCAGG
AGCATCAAGGTGGAGGCGGTGGAGGAGCCGGAGGCGGCCCCCATCCTGGGCCCCGGAGAG
CCTGGGCCCCAGGCCCCGTCGCGGACGCCGTCGCCGCGCAGCCCCGCCCCGGCCAGGGTC
AAGGCCGAGCTGTCCAGCCCCACGCCGGGCTCCAGCCCGGTGCCCGGCGAGCTGGGCCTG
GCCGGGGCCCTGTTCCTTCCGCAGTACGTGTTCGGGCCCGACGCGGCGCCCCCCGCCTCG
GAGATCCTGGCCAAGATGTCCGAGCTGGTGCACAGCCGGCTGCAGCAGGGCGCGGGCGCG
GGCGCCGGCGGCGCGCAGACCGGGCTCTTCCCCGGGGCCCCCAAGGGCGCTACGTGCTTC
GAGTGCGAGATCACCTTCAGCAACGTCAACAACTACTACGTGCACAAGCGCCTCTACTGT
TCAGGCCGCCGTGCGCCCGAGGACGCGCCTGCCGCGCGCAGGCCCAAGGCGCCCCCCGGC
CCGGCCCGCGCGCCCCCCGGCCAGCCCGCCGAACCCGACGCGCCGCGCTCGTCCCCGGGC
CCCGGAGCGCGCGAGGAGGGGGCTGGGGGCGCGGCCACGCCCGAGGACGGCGCGGGCGGC
CGGGGCAGCGAGGGCAGCCAGAGCCCGGGTAGCTCCGTGGACGACGCGGAGGACGACCCC
AGCCGCACGCTGTGCGAGGCCTGCAACATCCGCTTCAGCCGCCACGAGACCTACACCGTG
CACAAGCGGTACTACTGCGCCTCGCGCCACGACCCGCCGCCGCGCCGACCGGCCGCGCCC
CCGGGACCCCCTGGGCCGGCCGCGCCCCCGGCCCCCTCTCCCGCCGCGCCTGTGCGCACG
CGCAGACGCCGCAAGCTCTACGAGCTGCACGCGGCCGGCGCCCCGCCCCCCCCGCCGCCC
GGCCACGCCCCCGCGCCCGAGTCGCCGCGGCCCGGAAGCGGAAGCGGAAGCGGCCCCGGC
CTCGCCCCTGCGCGCTCGCCCGGCCCCGCGGCCGACGGCCCCATCGACCTGAGCAAGAAG
CCGCGGCGCCCGCTCCCCGGAGCCCCGGCACCGGCGCTGGCCGACTACCACGAGTGCACG
GCCTGCCGCGTGAGCTTCCACAGCCTCGAGGCCTACCTGGCGCACAAGAAGTACTCGTGC
CCCGCTGCGCCACCGCCCGGCGCGCTCGGCCTGCCCGCCGCCGCCTGCCCCTACTGCCCC
CCGAACGGCCCGGTGCGCGGGGACCTGCTGGAGCATTTCCGCCTGGCGCACGGCCTGCTG
CTCGGCGCGCCCCTGGCCGGCCCGGGGGTCGAGGCCCGGACGCCGGCCGACCGCGGCCCC
TCGCCCGCTCCCGCCCCCGCCGCCTCCCCGCAGCCCGGGTCCCGTGGCCCCCGGGACGGC
CTCGGCCCGGAGCCCCAGGAGCCGCCGCCCGGCCCGCCCCCGTCCCCGGCCGCCGCGCCC
GAGGCCGTGCCGCCCCCGCCGGCGCCCCCCTCCTACTCGGACAAGGGCGTCCAGACTCCC
AGCAAGGGCACGCCGGCGCCGCTGCCCAACGGCAACCACCGGTACTGCCGTCTTTGCAAC
ATCAAGTTCAGCAGCCTGTCCACCTTCATCGCCCACAAGAAGTATTACTGCTCCTCGCAC
GCCGCCGAGCACGTGAAGTTG
Protein sequence predicted based on nucleotide sequence
MSRRKQSNPRQIKRSLGDMEAREEVQLVGASHMEQKATAPEAPSPPSADVNSPPPLPPPT SPGGPKELEGQEPEPRPTEEEPGSPWSGPDELEPVVQDGQRRIRARLSLATGLSWGPFHG SVQTRASSPRQAEPSPALTLLLVDEACWLRTLPQALTEAEANTEIHRKDDALWCRVTKPV PAGGLLSVLLTAEPHSTPGHPVKKEPAEPTCPAPAHDLQLLPQQAGMASILATAVINKDV FPCKDCGIWYRSERNLQAHLLYYCASRQGTGSPAAAATDEKPKETYPNERVCPFPQCRKS CPSASSLEIHMRSHSGERPFVCLICLSAFTTKANCERHLKVHTDTLSGVCHSCGFISTTR DILYSHLVTNHMVCQPGSKGEIYSPGAGHPATKLPPDSLGSFQQQHTALQGPLASADLGL APTPSPGLDRKALAEATNGEARAEPLAQNGGSSEPPAAPRSIKVEAVEEPEAAPILGPGE PGPQAPSRTPSPRSPAPARVKAELSSPTPGSSPVPGELGLAGALFLPQYVFGPDAAPPAS EILAKMSELVHSRLQQGAGAGAGGAQTGLFPGAPKGATCFECEITFSNVNNYYVHKRLYC SGRRAPEDAPAARRPKAPPGPARAPPGQPAEPDAPRSSPGPGAREEGAGGAATPEDGAGG RGSEGSQSPGSSVDDAEDDPSRTLCEACNIRFSRHETYTVHKRYYCASRHDPPPRRPAAP PGPPGPAAPPAPSPAAPVRTRRRRKLYELHAAGAPPPPPPGHAPAPESPRPGSGSGSGPG LAPARSPGPAADGPIDLSKKPRRPLPGAPAPALADYHECTACRVSFHSLEAYLAHKKYSC PAAPPPGALGLPAAACPYCPPNGPVRGDLLEHFRLAHGLLLGAPLAGPGVEARTPADRGP SPAPAPAASPQPGSRGPRDGLGPEPQEPPPGPPPSPAAAPEAVPPPPAPPSYSDKGVQTP SKGTPAPLPNGNHRYCRLCNIKFSSLSTFIAHKKYYCSSHAAEHVKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
