Gene:
Gene Symbol:
ERCC6
Gene Name:
excision repair cross-complementing rodent repair deficiency, complementation group 6
Original Clone ID:
U8896CG250-532
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00945225
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 4482nts
Open reading frame: 1 to 4482
ATGCCAAATGAGGGAATCCCCCACTCAAGTCAAACTCAGGAGCAAGACTGTTTACAGAGT
CAACCTGTCAGTAATAATGAAGAAATGGCAATCAAGCAAGAAAGTGGTGGTGATGGGGAG
GTGGAGGAGTACCTCTCCTTTCGTTCTGTGGGTGACGGGCTGTCCACCTCTGCTGTGGGG
TGCGCATCAGCAGCTCCGAGGAGAGGGCCAGCCCTGCTGCACATCGACCGACATCAGATC
CAGGCAGTAGAGCCTAGCGCCCAGGCCCTTGAGCTGCAGGGTTTGGGTGTGGACGTCTAT
GACCAGGACGTGCTGGAACAGGGAGTGCTTCAGCAGGTGGACAATGCCATCCATGAGGCC
AGCCGTGCCTCCCAGCTCGTTGACGTGGAGAAGGAGTATCGGTCGGTCCTGGATGACCTC
ACGTCATGTACGACATCCCTAAGGCAAATCAATAAAATTATTGAACAGCTTAGCCCTCAA
GCTGCCACCAGCAGAGACATCAACAGGAAACTAGATTCTGTAAAACGACAGAAGTATAAT
AAGGAACAACAGCTAAAAAAGATCACTGCAAAACAAAAGCATCTCCAGGCCATCCTTGGA
GGAGCAGAGGTGAAAATTGAACTAGATCACGCCAGTCTGGAGGAGGATGCAGAGCCGGGG
CCATCCAGTCTTGGCAGCATGCTCATGCCTGTCCAGGAGACTGCCTGGGAAGAGCTCATC
CGCACTGGCCAGATGACACCTTTTGGTACCCAGATCCCTCAGAAACAGGAGAAAAAGCCC
AGAAAAATCATGCTTAATGAAGCATCAGGCTTCGAAAAGTATTTGGCAGATCAAGCAAAA
CTGTCTTTTGAAAGGAAGAAGCAAGGTTGTAATAAAAGAGCAGCTAGAAAAGCTCCAGCC
CCAGTCACGCCTCCAGCCCCAGTGCAAAATAAAAACAAACCAAACAAGAAAGCCAGAGTT
CTGTCCAAAAAAGAGGAGCGTTTGAAAAAGCACATCAAGAAACTCCAGAAGAGGGCTTTG
CAGTTCCAGGGGAAAGTGGGATTGCCAAAGGCAAGGAGACCTTGGGAGTCAGACATGAGG
CCAGAGGCAGAGGGAGACTCTGAGGGTGAAGAGTCTGAGTATTTCCCCACAGAGGAGGAG
GAAGAGGAGGAAGATGACGAGGTGGAGGGGGCAGAGGCGGACCTGTCTGGAGATGGTACT
GACTATGAGCTGAAGCCTCTGCCCAAGGGCGGGAAACGGCAGAAGAAAGTGCCAGTGCAG
GAGATTGATGATGACTTTTTCCCAAGTTCTGGGGAAGAAGCTGAAGCTGCTTCTGTAGGA
GAAGGAGGAGGAGGAGGTCGGAAAGTGGGAAGATACCGAGATGATGGAGATGAAGATTAT
TATAAGCAGCGGTTAAGGAGATGGAATAAACTGAGACTGCAGGACAAAGAGAAACGTCTG
AAGCTGGAGGACGATTCTGAGGAAAGTGATGCTGAATTTGACGAAGGTTTTAAAGTGCCA
GGTTTTCTGTTCAAAAAGCTTTTTAAGTACCAGCAGACAGGTGTTAGGTGGCTGTGGGAA
TTGCACTGCCAGCAGGCAGGAGGAATTCTGGGAGATGAAATGGGATTGGGCAAGACCATC
CAGATAATTGCCTTCTTGGCAGGTCTGAGCTACAGCAAGATCAGGACTCGTGGTTCAAAT
TACAGGTTTGAGGGGTTGGGTCCAACTGTAATTGTCTGTCCAACAACAGTGATGCATCAG
TGGGTGAAGGAATTTCACACGTGGTGGCCTCCGTTCAGAGTGGCAATTCTACATGAAACC
GGTTCCTATACCCACAAAAAGGAGAAACTAATTCGAGATGTTGCTCATTGTCATGGAATT
TTGATCACATCTTACTCCTACATTCGATTGATGCAGGATGACATTAGCAGGTATGACTGG
CACTATGTGATCTTGGACGAAGGACACAAAATTCGAAATCCAAATGCTGCTGTCACCCTT
GCTTGCAAACAGTTTCGCACCCCTCATCGGATCATTCTGTCTGGCTCACCGATGCAAAAT
AACCTCCGAGAGCTGTGGTCGCTCTTTGACTTCATCTTCCCGGGAAAGTTAGGCACGTTG
CCTGTGTTTATGGAGCAGTTCTCCGTCCCCATCACCATGGGGGGATATTCAAATGCTTCC
CCAGTACAGGTCAAAACTGCTTACAAGTGTGCATGTGTCTTACGAGATACCATAAATCCA
TACCTACTGCGGAGAATGAAGTCAGATGTCAAGATGAGCCTTTCTTTGCCAGATAAAAAT
GAACAGGTCTTATTTTGCCGTCTTACAGATGAGCAGCATAAAGTCTACCAAAATTTCGTT
GATTCCAAAGAAGTTTACAGGATTCTCAATGGAGAGATGCAGATTTTCTCCGGACTTATA
GCCCTAAGAAAAATTTGCAACCACCCTGATCTCTTTTCTGGAGGTCCCAAGAATCTCAAA
GGTCTTCCTGATGATGAACTAGAAGAAGATCAGTTTGGGTACTGGAAACGTTCTGGGAAA
ATGATTGTTGTTGAGTCTTTGTTGAAAATATGGCACAAGCAGGGTCAGCGAGTATTGCTG
TTTTCTCAGTCAAGGCAGATGCTGGACATACTTGAAGTATTCCTTAGAGCCCAAAAGTAT
ACCTATCTCAAGATGGATGGTACCACTACAATAGCTTCAAGACAGCCACTGATTACGAGA
TACAATGAGGACACATCCATATTTGTGTTTCTTCTGACCACGCGGGTGGGCGGCTTAGGT
GTCAACCTGACGGGGGCAAACAGAGTTGTCATCTATGACCCAGACTGGAACCCAAGCACG
GACACGCAGGCCCGGGAGCGAGCATGGAGAATAGGCCAGAAGAAGCAAGTGACTGTGTAC
AGGCTCCTGACTGCGGGCACCATTGAAGAAAAGATCTACCACCGACAAATCTTCAAGCAG
TTTTTGACAAATAGAGTGCTAAAAGACCCAAAACAAAGGCGGTTTTTCAAATCCAATGAT
CTCTATGAGCTATTTACTCTGACTAGTCCTGATGCATCCCAGAGCACTGAAACAAGTGCA
ATTTTTGCAGGAACTGGATCAGATGTTCAGACACCCAAATGCCATCTAAAAAGAAGGATT
CAACCAGCCTTTGGAGCAGACCATGATGTTCCAAAACGCAAGAAGTTCCCTGCTTCTAAC
ATATCTGTAAATGATGCCACATCATCTGAAGAGAAATCTGAGGCTAAAGGAGCTGAAGTA
AATGCAGTAACTTCTAATCGAAGTGATCCTTTGAAAGATGACCCTCACATGAGTAGTAAT
GTAACTAGCAATGATAGGCTTGGAGAAGAGACAAATGCAGTATCTGGACCAGAAGAGTTG
TCAGTGATTAGTGGAAATGGGGAATGTTCAAATTCTTCAGGAACAGGCAAAACTTCTATG
CCATCTGGTGATGAAAGCATTGATGAAAAGTTAGGTCTTTCTTACAAAAGAGAAAGACCC
AGCCAGGCTCAAACAGAAGCTTTTTGGGAGAATAAACAAATGGAAAATAATTTTTATAAG
CACAAGTCAAAAACAAAACATCATAGTGTGGCAGAAGAAGAGACCCTGGAGAAACATCTG
AGACCAAAGCAAAAGCCTAAGAACTCTAAGCATTGCAGAGACGCCAAGTTTGAAGGAACT
CGAATTCCACACCTGGTGAAGAAAAGGCGTTACCAGAAGCAAGACAGTGAAAACAAGAGT
GAGGCCAAGGAACAGAGCAATGACGATTATGTTTTGGAAAAGCTTTTCAAAAAATCAGTT
GGCGTGCACAGTGTCATGAAGCACGATGCCATCATGGATGGAGCCAGCCCAGATTATGTA
CTGGTGGAGGCAGAAGCCAACCGAGTGGCCCAGGATGCCCTGAAAGCACTGAGGCTCTCT
CGTCAGCGGTGTCTGGGAGCAGTGTCTGGTGTTCCCACCTGGACTGGCCACAGGGGGATT
TCTGGTGCACCAGCAGGAAAAAAGAGTAGATTTGGTAAGAAAAGGAATTCTAACTTCTCT
GTGCAGCATCCTTCATCAACATCTCCAACAGAGAAGTGCCAGGATGGCATCATGAAAAAG
GAGGGAAAAGATAATGTCCCTGAGCATTTTAGTGGAAGAGCAGAAGATGCAGACTCTTCA
TCCGGGCCCCTCGCTTCCTCCTCACTCTTGGCTAAAATGAGAGCTAGAAACCACCTGATT
CTGCCAGAGCGTTTAGAAAGTGAAAGCGGGCACCTGCAGGAAGCTTCTGCCCTGCTGCCC
ACCACAGAACACGATGACCTTCTGGTGGAGATGAGAAACTTCATCGCTTTCCAGGCCCAC
ACTGATGGCCAGGCCAGCACCAGGGAGATACTGCAGGAGTTTGAATCCAAGTTATCTGCA
TCACAGTCTTGTGTCTTCCGAGAACTATTGAGAAATCTGTGCACTTTCCATAGAACTTCT
GGTGGTGAAGGAATTTGGAAACTCAAGCCAGAATACTGCTTG
Protein sequence predicted based on nucleotide sequence
MPNEGIPHSSQTQEQDCLQSQPVSNNEEMAIKQESGGDGEVEEYLSFRSVGDGLSTSAVG CASAAPRRGPALLHIDRHQIQAVEPSAQALELQGLGVDVYDQDVLEQGVLQQVDNAIHEA SRASQLVDVEKEYRSVLDDLTSCTTSLRQINKIIEQLSPQAATSRDINRKLDSVKRQKYN KEQQLKKITAKQKHLQAILGGAEVKIELDHASLEEDAEPGPSSLGSMLMPVQETAWEELI RTGQMTPFGTQIPQKQEKKPRKIMLNEASGFEKYLADQAKLSFERKKQGCNKRAARKAPA PVTPPAPVQNKNKPNKKARVLSKKEERLKKHIKKLQKRALQFQGKVGLPKARRPWESDMR PEAEGDSEGEESEYFPTEEEEEEEDDEVEGAEADLSGDGTDYELKPLPKGGKRQKKVPVQ EIDDDFFPSSGEEAEAASVGEGGGGGRKVGRYRDDGDEDYYKQRLRRWNKLRLQDKEKRL KLEDDSEESDAEFDEGFKVPGFLFKKLFKYQQTGVRWLWELHCQQAGGILGDEMGLGKTI QIIAFLAGLSYSKIRTRGSNYRFEGLGPTVIVCPTTVMHQWVKEFHTWWPPFRVAILHET GSYTHKKEKLIRDVAHCHGILITSYSYIRLMQDDISRYDWHYVILDEGHKIRNPNAAVTL ACKQFRTPHRIILSGSPMQNNLRELWSLFDFIFPGKLGTLPVFMEQFSVPITMGGYSNAS PVQVKTAYKCACVLRDTINPYLLRRMKSDVKMSLSLPDKNEQVLFCRLTDEQHKVYQNFV DSKEVYRILNGEMQIFSGLIALRKICNHPDLFSGGPKNLKGLPDDELEEDQFGYWKRSGK MIVVESLLKIWHKQGQRVLLFSQSRQMLDILEVFLRAQKYTYLKMDGTTTIASRQPLITR YNEDTSIFVFLLTTRVGGLGVNLTGANRVVIYDPDWNPSTDTQARERAWRIGQKKQVTVY RLLTAGTIEEKIYHRQIFKQFLTNRVLKDPKQRRFFKSNDLYELFTLTSPDASQSTETSA IFAGTGSDVQTPKCHLKRRIQPAFGADHDVPKRKKFPASNISVNDATSSEEKSEAKGAEV NAVTSNRSDPLKDDPHMSSNVTSNDRLGEETNAVSGPEELSVISGNGECSNSSGTGKTSM PSGDESIDEKLGLSYKRERPSQAQTEAFWENKQMENNFYKHKSKTKHHSVAEEETLEKHL RPKQKPKNSKHCRDAKFEGTRIPHLVKKRRYQKQDSENKSEAKEQSNDDYVLEKLFKKSV GVHSVMKHDAIMDGASPDYVLVEAEANRVAQDALKALRLSRQRCLGAVSGVPTWTGHRGI SGAPAGKKSRFGKKRNSNFSVQHPSSTSPTEKCQDGIMKKEGKDNVPEHFSGRAEDADSS SGPLASSSLLAKMRARNHLILPERLESESGHLQEASALLPTTEHDDLLVEMRNFIAFQAH TDGQASTREILQEFESKLSASQSCVFRELLRNLCTFHRTSGGEGIWKLKPEYCL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
