Gene:
Gene Symbol:
LPHN2
Gene Name:
latrophilin 2
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Genentech clones
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00945531
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4377nts
Open reading frame: 1 to 4377
ATGGTCAGCTCCGGTTGCCGTATGCGGAGCCTGTGGTTTATCATTGTGATCAGTTTCCTG
CCTAACACAGAGGGCTTCAGCCGGGCCGCCCTGCCATTCGGCCTGGTGCGGAGAGAGCTG
AGTTGCGAAGGGTACTCAATCGACCTGCGCTGTCCAGGCAGCGATGTGATCATGATTGAA
TCTGCAAATTATGGCCGAACCGACGATAAGATTTGCGACGCCGATCCCTTTCAGATGGAG
AACACCGACTGTTACCTGCCTGATGCCTTCAAAATCATGACACAGAGGTGCAACAATCGC
ACTCAGTGTATTGTGGTCACCGGCAGCGACGTGTTTCCCGACCCCTGTCCTGGCACATAT
AAGTACCTGGAGGTCCAGTATGAATGCGTGCCCTACATCTTCGTGTGCCCTGGAACTCTG
AAAGCTATCGTGGACTCCCCATGCATCTACGAGGCAGAACAGAAAGCAGGCGCCTGGTGT
AAGGACCCCCTGCAGGCAGCCGATAAAATCTACTTTATGCCCTGGACACCGTACAGAACT
GACACCCTGATTGAGTATGCCAGCCTGGAGGATTTCCAGAACAGCCGGCAGACCACAACT
TACAAGCTGCCCAACCGCGTGGACGGAACCGGTTTTGTGGTGTACGATGGGGCCGTCTTC
TTTAACAAGGAAAGGACACGCAACATCGTTAAGTTCGACCTGCGAACTCGGATCAAGTCA
GGAGAGGCCATCATTAATTATGCTAACTACCACGACACTAGCCCATACCGTTGGGGTGGG
AAGACCGACATCGATCTGGCCGTGGACGAAAATGGCCTGTGGGTCATCTACGCTACTGAG
CAGAACAATGGAATGATCGTGATTTCCCAGCTGAACCCTTACACCCTCAGGTTTGAGGCA
ACCTGGGAAACAGTGTATGATAAGCGCGCTGCATCCAATGCCTTCATGATCTGCGGCGTC
CTGTATGTGGTGCGGAGCGTGTACCAGGACAATGAGTCAGAAACTGGCAAGAACAGCATC
GATTATATCTACAATACCCGGCTGAACAGAGGAGAGTACGTGGACGTGCCATTTCCGAAC
CAGTATCAGTACATCGCCGCTGTGGACTATAATCCCAGAGATAACCAGCTGTACGTCTGG
AACAATAACTTTATTCTCAGGTATAGCCTGGAGTTCGGTCCACCTGATCCCGCTCAGGTC
CCTACCACAGCAGTTACAATCACTTCTAGTGCCGAGCTGTTCAAGACCATCATTAGTACT
ACCTCAACAACTAGCCAGAAAGGGCCTATGTCTACCACAGTCGCTGGAAGCCAGGAGGGC
TCCAAGGGGACAAAACCACCGCCCGCAGTGAGCACTACCAAGATCCCTCCAATTACTAAC
ATCTTTCCCCTGCCTGAGCGGTTCTGCGAAGCCCTGGACAGCAAGGGCATCAAGTGGCCA
CAGACCCAGAGGGGCATGATGGTGGAGCGCCCATGCCCAAAGGGCACCCGGGGCACCGCT
TCCTACCTGTGCATGATCTCTACCGGCACCTGGAATCCCAAGGGCCCTGATCTGTCTAAT
TGTACTAGTCATTGGGTGAACCAGCTGGCCCAGAAAATTAGGAGTGGGGAAAATGCAGCC
AGCCTGGCCAACGAGCTGGCAAAGCACACCAAAGGCCCTGTCTTCGCTGGCGACGTGAGC
AGCAGCGTGCGGCTCATGGAACAGCTGGTGGACATCCTGGACGCCCAGCTCCAGGAGCTG
AAACCATCAGAAAAGGATAGCGCCGGCCGCTCCTACAACAAGCTGCAGAAACGGGAGAAG
ACATGCAGAGCCTATCTGAAGGCTATCGTGGACACTGTCGATAATCTGCTGCGGCCCGAG
GCCCTGGAATCCTGGAAACACATGAACTCTAGTGAGCAGGCCCATACTGCTACCATGCTG
CTGGACACCCTGGAGGAAGGTGCCTTCGTGCTGGCCGATAACCTGCTGGAGCCAACAAGA
GTCAGTATGCCCACTGAGAACATCGTGCTGGAGGTTGCCGTGCTGAGCACCGAGGGGCAG
ATTCAGGACTTTAAATTCCCTCTGGGAATCAAGGGCGCCGGGTCAAGCATTCAGCTGTCA
GCTAACACAGTTAAGCAGAATAGCCGAAACGGCCTGGCCAAACTGGTGTTTATCATCTAC
CGGAGCCTGGGCCAGTTCCTGAGCACCGAAAACGCCACCATCAAGCTGGGAGCTGACTTT
ATCGGTCGGAACAGCACCATTGCCGTGAACAGTCATGTGATCAGCGTGAGCATCAACAAG
GAGTCCTCTCGCGTGTACCTGACCGACCCCGTGCTGTTCACCCTGCCACACATCGACCCG
GATAATTATTTTAATGCCAACTGCAGCTTCTGGAACTATTCCGAGCGGACCATGATGGGG
TACTGGTCTACACAGGGATGTAAACTGGTGGACACAAATAAGACTCGGACAACTTGCGCC
TGTAGCCACCTGACCAACTTTGCTATCCTCATGGCACATAGAGAAATTGCTTACAAGGAT
GGGGTGCACGAGCTGCTCCTGACCGTCATCACATGGGTCGGAATCGTGATCAGCCTGGTG
TGCCTGGCCATCTGTATTTTTACTTTCTGCTTCTTTCGAGGGCTGCAGTCCGACCGTAAC
ACCATTCATAAGAACCTGTGCATTAACCTGTTCATCGCTGAGTTTATTTTCCTGATCGGC
ATTGATAAGACCAAATACGCTATCGCATGCCCAATTTTTGCTGGCCTGCTGCACTTCTTT
TTCCTGGCTGCATTCGCATGGATGTGCCTGGAGGGCGTGCAGCTCTATCTGATGCTCGTC
GAGGTTTTTGAGTCCGAATACTCTAGGAAGAAATACTATTACGTCGCAGGGTATCTGTTC
CCAGCAACCGTGGTCGGAGTGAGCGCCGCTATCGACTATAAATCCTACGGGACAGAGAAG
GCCTGCTGGCTGCATGTCGATAATTACTTTATCTGGAGCTTCATTGGACCAGTGACCTTC
ATCATTCTGCTGAACATCATCTTCCTGGTCATCACCCTGTGCAAAATGGTGAAGCACAGC
AATACACTGAAGCCCGACAGCAGCCGGCTGGAGAACATCAAGTCCTGGGTGCTGGGTGCC
TTCGCTCTCCTGTGCCTCCTGGGCCTGACCTGGAGCTTCGGCCTGCTGTTCATCAATGAG
GAAACTATTGTGATGGCATACCTGTTCACCATCTTTAACGCCTTCCAGGGCGTCTTTATC
TTCATTTTTCACTGCGCCCTGCAGAAGAAAGTGCGGAAAGAATATGGGAAGTGTTTTAGA
CATAGCTACTGCTGTGGAGGCCTGCCCACAGAGTCCCCTCACAGCTCCGTGAAGGCTTCT
ACCACACGAACAAGTGCACGTTACTCTAGTGGCACTCAGAGCCGAATCAGGCGCATGTGG
AACGACACCGTGCGGAAACAGTCAGAGAGCAGCTTCATCAGCGGGGATATTAATTCTACA
AGTACTCTGAACCAGGGCATGACTGGGAATTACCTGCTGACCAACCCACTCCTGCGGCCC
CACGGCACCAATAACCCTTATAATACACTGCTGGCCGAGACTGTGGTGTGCAATGCACCA
AGCGCCCCCGTGTTCAACTCTCCTGGCCATAGTCTGAATAACGCACGCGACACCAGCGCA
ATGGACACACTGCCCCTGAACGGTAACTTCAATAACTCATATAGCCTGCACAAGGGCGAC
TACAATGATTCCGTGCAGGTCGTGGACTGTGGCCTGAGCCTGAACGATACCGCCTTCGAA
AAAATGATCATTTCTGAGCTGGTGCATAATAACCTGAGGGGCTCCTCTAAGACACACAAC
CTGGAGCTGACCCTGCCCGTGAAACCTGTCATCGGCGGGAGTTCAAGCGAGGACGATGCA
ATTGTGGCAGACGCCTCCTCTCTGATGCATAGCGATAATCCCGGCCTGGAGCTGCACCAT
AAGGAGCTGGAAGCTCCACTCATCCCGCAGCGCACACACTCTCTCCTGTACCAGCCTCAG
AAGAAAGTTAAAAGTGAGGGAACCGACAGCTACGTGAGCCAGCTGACAGCTGAGGCAGAA
GACCATCTGCAGTCTCCAAATCGGGATTCACTGTACACCAGCATGCCCAACCTGAGAGAC
AGCCCCTATCCTGAGAGTTCACCTGACATGGAGGAGGACCTGTCCCCCAGCCGGCGGAGC
GAGAATGAAGACATCTACTATAAGTCAATGCCCAACCTGGGAGCCGGTCACCAGCTCCAG
ATGTGCTATCAGATTTCCCGAGGAAATTCTGACGGTTACATCATTCCCATCAACAAGGAG
GGCTGTATTCCTGAAGGGGATGTGAGAGAGGGACAGATGCAGCTGGTGACCAGCCTG
Protein sequence predicted based on nucleotide sequence
MVSSGCRMRSLWFIIVISFLPNTEGFSRAALPFGLVRRELSCEGYSIDLRCPGSDVIMIE SANYGRTDDKICDADPFQMENTDCYLPDAFKIMTQRCNNRTQCIVVTGSDVFPDPCPGTY KYLEVQYECVPYIFVCPGTLKAIVDSPCIYEAEQKAGAWCKDPLQAADKIYFMPWTPYRT DTLIEYASLEDFQNSRQTTTYKLPNRVDGTGFVVYDGAVFFNKERTRNIVKFDLRTRIKS GEAIINYANYHDTSPYRWGGKTDIDLAVDENGLWVIYATEQNNGMIVISQLNPYTLRFEA TWETVYDKRAASNAFMICGVLYVVRSVYQDNESETGKNSIDYIYNTRLNRGEYVDVPFPN QYQYIAAVDYNPRDNQLYVWNNNFILRYSLEFGPPDPAQVPTTAVTITSSAELFKTIIST TSTTSQKGPMSTTVAGSQEGSKGTKPPPAVSTTKIPPITNIFPLPERFCEALDSKGIKWP QTQRGMMVERPCPKGTRGTASYLCMISTGTWNPKGPDLSNCTSHWVNQLAQKIRSGENAA SLANELAKHTKGPVFAGDVSSSVRLMEQLVDILDAQLQELKPSEKDSAGRSYNKLQKREK TCRAYLKAIVDTVDNLLRPEALESWKHMNSSEQAHTATMLLDTLEEGAFVLADNLLEPTR VSMPTENIVLEVAVLSTEGQIQDFKFPLGIKGAGSSIQLSANTVKQNSRNGLAKLVFIIY RSLGQFLSTENATIKLGADFIGRNSTIAVNSHVISVSINKESSRVYLTDPVLFTLPHIDP DNYFNANCSFWNYSERTMMGYWSTQGCKLVDTNKTRTTCACSHLTNFAILMAHREIAYKD GVHELLLTVITWVGIVISLVCLAICIFTFCFFRGLQSDRNTIHKNLCINLFIAEFIFLIG IDKTKYAIACPIFAGLLHFFFLAAFAWMCLEGVQLYLMLVEVFESEYSRKKYYYVAGYLF PATVVGVSAAIDYKSYGTEKACWLHVDNYFIWSFIGPVTFIILLNIIFLVITLCKMVKHS NTLKPDSSRLENIKSWVLGAFALLCLLGLTWSFGLLFINEETIVMAYLFTIFNAFQGVFI FIFHCALQKKVRKEYGKCFRHSYCCGGLPTESPHSSVKASTTRTSARYSSGTQSRIRRMW NDTVRKQSESSFISGDINSTSTLNQGMTGNYLLTNPLLRPHGTNNPYNTLLAETVVCNAP SAPVFNSPGHSLNNARDTSAMDTLPLNGNFNNSYSLHKGDYNDSVQVVDCGLSLNDTAFE KMIISELVHNNLRGSSKTHNLELTLPVKPVIGGSSSEDDAIVADASSLMHSDNPGLELHH KELEAPLIPQRTHSLLYQPQKKVKSEGTDSYVSQLTAEAEDHLQSPNRDSLYTSMPNLRD SPYPESSPDMEEDLSPSRRSENEDIYYKSMPNLGAGHQLQMCYQISRGNSDGYIIPINKE GCIPEGDVREGQMQLVTSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
