CASR (Homo sapiens) in pJFT7_nHalo_DC(r4)

Explanation of Terms

Gene:
Gene Symbol:  CASR
Gene Name:  calcium-sensing receptor
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format: 
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Genentech clones
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00946161
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AVAILABLE
No restriction
Special MTA: HALO
Insert sequence: 3234nts
Open reading frame: 1 to 3234

ATGGCCTTCTACAGCTGCTGTTGGGTGCTGCTGGCCCTGACCTGGCACACATCCGCTTAT
GGCCCCGATCAGCGGGCACAGAAGAAAGGGGACATCATTCTCGGCGGGCTGTTTCCAATT
CATTTCGGAGTCGCCGCTAAGGATCAGGACCTGAAAAGTCGGCCCGAGTCAGTGGAATGC
ATCAGATACAATTTTAGGGGCTTCCGCTGGCTGCAGGCTATGATCTTTGCAATTGAGGAA
ATCAATAGCTCCCCTGCCCTGCTCCCAAACCTGACCCTGGGCTATAGAATTTTCGATACT
TGTAACACCGTGTCAAAGGCCCTGGAGGCTACCCTGAGCTTTGTCGCTCAGAACAAAATC
GATAGCCTGAACCTGGACGAGTTCTGCAACTGTAGTGAACACATTCCCTCAACCATCGCA
GTGGTCGGAGCTACTGGCAGCGGCGTGAGCACCGCAGTGGCTAACCTGCTCGGCCTGTTC
TACATTCCCCAGGTGTCCTATGCCTCTAGTTCAAGACTGCTCTCAAACAAGAATCAGTTC
AAGAGCTTCCTGAGGACAATCCCTAATGATGAACATCAGGCAACTGCTATGGCCGACATC
ATTGAGTACTTCCGCTGGAACTGGGTGGGCACCATTGCAGCCGACGATGACTATGGGCGA
CCTGGAATCGAGAAGTTTCGAGAGGAAGCCGAGGAACGTGACATCTGCATTGACTTCTCT
GAACTGATTAGTCAGTACTCAGATGAGGAAGAGATTCAGCACGTTGTGGAAGTGATCCAG
AATAGCACTGCCAAAGTGATCGTGGTGTTCAGCAGCGGTCCCGACCTGGAGCCCCTGATT
AAGGAAATCGTGCGGAGAAACATTACAGGAAAAATCTGGCTGGCTTCTGAGGCATGGGCC
TCTAGTTCACTGATCGCAATGCCACAGTATTTTCATGTGGTCGGAGGCACCATTGGGTTC
GCACTGAAGGCCGGTCAGATCCCAGGCTTTCGAGAGTTCCTGAAGAAAGTCCACCCGCGT
AAGAGCGTTCATAATGGATTTGCAAAAGAGTTCTGGGAAGAGACATTCAACTGCCACCTG
CAGGAGGGTGCAAAGGGTCCACTCCCGGTGGATACATTCCTGAGGGGCCATGAAGAGAGC
GGGGACCGCTTCTCCAATAGCTCCACTGCCTTTCGGCCACTGTGCACCGGCGATGAGAAC
ATCTCTAGTGTGGAAACACCTTACATTGACTATACTCACCTGAGAATCTCCTACAACGTC
TATCTGGCCGTGTACTCTATTGCTCATGCACTGCAGGACATCTACACCTGCCTCCCCGGG
AGGGGACTGTTCACAAATGGCTCTTGTGCCGACATCAAGAAAGTTGAGGCTTGGCAGGTG
CTGAAGCACCTGCGCCACCTGAACTTTACAAACAATATGGGGGAGCAAGTGACTTTCGAT
GAATGCGGGGACCTGGTCGGAAACTACAGCATCATTAATTGGCACCTGTCTCCAGAGGAC
GGAAGTATCGTGTTTAAGGAAGTCGGTTACTATAACGTGTACGCCAAGAAAGGCGAGCGG
CTGTTCATCAACGAAGAGAAAATCCTGTGGAGCGGGTTCTCCCGCGAGGTGCCCTTTTCT
AATTGCAGTAGGGATTGTCTGGCCGGCACCCGCAAGGGCATCATTGAAGGGGAGCCAACC
TGCTGTTTTGAGTGCGTGGAATGTCCGGACGGAGAGTACTCCGATGAAACAGACGCCTCT
GCTTGCAACAAGTGTCCAGATGACTTCTGGAGTAACGAAAATCATACTTCATGCATTGCC
AAAGAAATCGAGTTCCTGAGCTGGACCGAGCCCTTTGGGATCGCCCTGACCCTGTTCGCC
GTGCTGGGAATCTTTCTGACCGCCTTCGTGCTGGGCGTGTTCATCAAGTTCCGGAATACC
CCTATCGTGAAAGCAACAAACCGGGAACTGAGCTATCTGCTCCTGTTCTCCCTCCTGTGC
TGTTTTTCAAGCTCCCTGTTCTTTATTGGAGAGCCCCAGGATTGGACCTGCCGGCTGCGT
CAGCCTGCCTTCGGCATCAGCTTCGTGCTGTGCATTAGTTGTATCCTGGTTAAGACCAAT
CGGGTGCTGCTGGTGTTCGAGGCTAAGATCCCCACCAGCTTCCACAGAAAATGGTGGGGC
CTGAACCTGCAGTTTCTCCTGGTGTTCCTGTGCACTTTTATGCAGATCGTGATCTGCGTG
ATCTGGCTGTACACCGCACCCCCTTCTAGTTATCGGAATCAGGAACTGGAGGACGAAATC
ATTTTTATCACATGCCATGAGGGGTCCCTGATGGCTCTCGGATTCCTGATTGGTTACACT
TGCCTGCTGGCCGCAATCTGTTTCTTTTTCGCCTTTAAGAGCAGAAAACTGCCTGAGAAC
TTCAATGAGGCTAAGTTTATCACCTTCTCCATGCTGATCTTCTTCATCGTGTGGATTTCC
TTTATCCCAGCCTACGCCAGCACCTACGGCAAGTTCGTGAGTGCTGTGGAGGTCATCGCA
ATTCTGGCAGCCAGCTTCGGCCTGCTGGCCTGCATCTTCTTCAACAAAATCTACATCATC
CTGTTCAAACCTAGTCGAAACACTATCGAAGAGGTGCGGTGTAGCACCGCAGCACACGCC
TTCAAGGTCGCTGCAAGGGCCACCCTGAGGCGCTCAAATGTGAGCCGGAAAAGATCAAGC
TCCCTGGGCGGGTCCACAGGCTCTACTCCCTCTAGTTCAATCAGCTCCAAGTCTAACAGT
GAGGACCCCTTCCCTCAGCCAGAACGCCAGAAACAGCAGCAGCCCCTGGCCCTGACACAG
CAGGAGCAGCAGCAGCAGCCTCTGACCCTGCCACAGCAGCAGCGGAGCCAGCAGCAGCCT
AGATGCAAGCAGAAAGTGATCTTCGGCAGCGGCACCGTCACATTCTCACTGAGCTTTGAT
GAACCACAGAAGAACGCTATGGCACACAGGAACTCAACCCATCAGAACAGCCTGGAGGCC
CAGAAATCTAGTGACACTCTGACCCGTCACGAGCCACTCCTGCCACTGCAGTGTGGCGAG
ACAGACCTGGACCTGACCGTGCAGGAGACAGGGCTGCAGGGACCAGTGGGAGGCGACCAG
CGGCCCGAGGTCGAAGACCCCGAAGAGCTGAGCCCTGCCCTGGTGGTGAGCAGCAGCCAG
AGCTTCGTGATCAGCGGCGGGGGATCTACAGTCACTGAGAACGTCGTGAACAGC

Protein sequence predicted based on nucleotide sequence

MAFYSCCWVLLALTWHTSAYGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVEC
IRYNFRGFRWLQAMIFAIEEINSSPALLPNLTLGYRIFDTCNTVSKALEATLSFVAQNKI
DSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQF
KSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKFREEAEERDICIDFS
ELISQYSDEEEIQHVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGKIWLASEAWA
SSSLIAMPQYFHVVGGTIGFALKAGQIPGFREFLKKVHPRKSVHNGFAKEFWEETFNCHL
QEGAKGPLPVDTFLRGHEESGDRFSNSSTAFRPLCTGDENISSVETPYIDYTHLRISYNV
YLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHLNFTNNMGEQVTFD
ECGDLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEEKILWSGFSREVPFS
NCSRDCLAGTRKGIIEGEPTCCFECVECPDGEYSDETDASACNKCPDDFWSNENHTSCIA
KEIEFLSWTEPFGIALTLFAVLGIFLTAFVLGVFIKFRNTPIVKATNRELSYLLLFSLLC
CFSSSLFFIGEPQDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSFHRKWWG
LNLQFLLVFLCTFMQIVICVIWLYTAPPSSYRNQELEDEIIFITCHEGSLMALGFLIGYT
CLLAAICFFFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIA
ILAASFGLLACIFFNKIYIILFKPSRNTIEEVRCSTAAHAFKVAARATLRRSNVSRKRSS
SLGGSTGSTPSSSISSKSNSEDPFPQPERQKQQQPLALTQQEQQQQPLTLPQQQRSQQQP
RCKQKVIFGSGTVTFSLSFDEPQKNAMAHRNSTHQNSLEAQKSSDTLTRHEPLLPLQCGE
TDLDLTVQETGLQGPVGGDQRPEVEDPEELSPALVVSSSQSFVISGGGSTVTENVVNS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable