Gene:
Gene Symbol:
MYH6
Gene Name:
myosin, heavy chain 6, cardiac muscle, alpha
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00946193
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5820nts
Open reading frame: 1 to 5820
ATGACCGATGCCCAGATGGCTGACTTTGGGGCAGCGGCCCAGTACCTCCGCAAGTCAGAG
AAGGAGCGTCTAGAGGCCCAGACCCGGCCCTTTGACATTCGCACTGAGTGCTTCGTGCCC
GATGACAAGGAAGAGTTTGTCAAAGCCAAGATTTTGTCCCGGGAGGGAGGCAAGGTCATT
GCTGAAACCGAGAATGGGAAGACGGTGACTGTGAAGGAGGACCAGGTGTTGCAGCAGAAC
CCACCCAAGTTCGACAAGATTGAGGACATGGCCATGCTGACCTTCCTGCACGAGCCCGCG
GTGCTTTTCAACCTCAAGGAGCGCTACGCGGCCTGGATGATATATACCTACTCGGGCCTC
TTCTGTGTCACTGTCAACCCCTACAAGTGGCTGCCGGTGTACAATGCCGAGGTGGTGGCC
GCCTACCGGGGCAAGAAGAGGAGTGAGGCCCCGCCCCACATCTTCTCCATCTCCGACAAC
GCCTATCAGTACATGCTGACAGATCGGGAGAACCAGTCCATCCTCATCACGGGAGAATCC
GGGGCGGGGAAGACTGTGAACACCAAGCGTGTCATCCAGTACTTTGCCAGCATTGCAGCC
ATAGGTGACCGTGGCAAGAAGGACAATGCCAATGCGAACAAGGGCACCCTGGAGGACCAG
ATCATCCAGGCCAACCCCGCTCTGGAGGCCTTCGGCAATGCCAAGACTGTCCGGAACGAC
AACTCCTCCCGCTTTGGGAAATTCATTAGGATCCACTTTGGGGCCACTGGAAAGCTGGCT
TCTGCAGACATAGAGACCTACCTGCTGGAGAAGTCCCGGGTGATCTTCCAGCTGAAAGCT
GAGAGAAACTACCACATCTTCTACCAGATTCTGTCCAACAAGAAGCCGGAGTTGCTGGAC
ATGCTGCTGGTCACCAACAATCCCTACGACTACGCCTTCGTGTCTCAGGGAGAGGTGTCC
GTGGCCTCCATTGATGACTCCGAGGAGCTCATGGCCACCGATAGTGCCTTTGACGTGCTG
GGCTTCACTTCAGAGGAGAAAGCTGGCGTCTACAAGCTGACGGGAGCCATCATGCACTAC
GGGAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGGCGGAGCCAGACGGCACCGAA
GATGCTGACAAGTCGGCCTACCTCATGGGGCTGAACTCAGCTGACCTGCTCAAGGGGCTG
TGCCACCCTCGGGTGAAAGTGGGCAACGAGTATGTCACCAAGGGGCAGAGCGTGCAGCAG
GTGTACTACTCCATCGGGGCTCTGGCCAAGGCAGTGTATGAGAAGATGTTCAACTGGATG
GTGACGCGCATCAACGCCACCCTGGAGACCAAGCAGCCACGCCAGTACTTCATAGGAGTC
CTGGACATCGCTGGCTTCGAGATCTTCGACTTCAACAGCTTTGAGCAGCTCTGCATCAAC
TTCACCAACGAGAAGCTGCAGCAGTTCTTCAACCACCACATGTTCGTGCTGGAGCAGGAG
GAGTACAAGAAGGAGGGCATTGAGTGGACATTCATTGACTTTGGCATGGACCTGCAGGCC
TGCATTGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTGGAGGAGGAGTGCATG
TTCCCCAAGGCCACTGACATGACCTTCAAGGCCAAGCTGTACGACAACCACCTGGGCAAG
TCCAACAATTTCCAGAAGCCACGCAACATCAAGGGGAAGCAGGAAGCCCACTTCTCCCTG
ATCCACTACGCCGGCACTGTGGACTACAACATCCTGGGCTGGCTGGAAAAAAACAAGGAT
CCTCTCAACGAGACTGTTGTGGCCCTGTACCAGAAGTCCTCCCTCAAGCTCATGGCCACT
CTCTTCTCCTCCTACGCAACTGCCGATACTGGGGACAGTGGTAAAAGCAAAGGAGGCAAG
AAAAAGGGCTCATCCTTCCAGACGGTGTCGGCTCTCCACCGGGAAAATCTCAACAAGCTA
ATGACCAACCTGAGGACCACCCATCCTCACTTTGTGCGTTGCATCATCCCCAATGAGCGG
AAGGCTCCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGCTGCAATGGCGTG
CTGGAGGGCATCCGCATCTGCAGGAAGGGCTTCCCCAACCGCATCCTCTACGGGGACTTC
CGGCAGAGGTATCGCATCCTGAACCCAGTGGCCATCCCTGAGGGACAGTTCATTGATAGC
AGGAAGGGGACAGAGAAGCTGCTCAGCTCTCTGGACATTGATCACAACCAGTACAAGTTT
GGCCACACCAAGGTGTTCTTCAAGGCAGGGCTGCTTGGGCTGCTGGAGGAGATGCGGGAT
GAGAGGCTGAGCCGCATCATCACGCGCATGCAGGCCCAAGCCCGGGGCCAGCTCATGCGC
ATTGAGTTCAAGAAGATAGTGGAACGCAGGGATGCCCTGCTGGTAATCCAGTGGAACATT
CGGGCCTTCATGGGGGTCAAGAATTGGCCCTGGATGAAGCTCTACTTCAAGATCAAGCCG
CTGCTGAAGAGCGCAGAGACGGAGAAGGAGATGGCCACCATGAAGGAAGAGTTCGGGCGC
ATCAAAGAGACGCTGGAGAAGTCCGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTG
TCCCTGCTGCAGGAGAAGAATGACCTGCAGCTCCAAGTGCAGGCGGAACAAGACAACCTC
AATGATGCTGAGGAGCGCTGCGACCAGCTGATCAAAAACAAGATTCAGCTGGAGGCCAAA
GTAAAGGAGATGAATGAGAGGCTGGAGGATGAGGAGGAGATGAACGCGGAGCTCACTGCC
AAGAAGCGCAAGCTGGAAGACGAGTGCTCAGAGCTCAAGAAGGACATTGATGACCTGGAG
CTGACACTGGCCAAGGTGGAGAAGGAGAAGCATGCAACAGAGAACAAGGTGAAGAACCTA
ACAGAGGAGATGGCTGGGCTGGATGAAATCATCGCTAAGCTGACCAAGGAGAAGAAAGCT
CTACAAGAGGCCCATCAGCAGGCCCTGGATGACCTTCAGGTTGAGGAAGACAAGGTCAAC
AGCCTGTCCAAGTCTAAGGTCAAGCTGGAGCAGCAGGTGGATGATCTGGAGGGATCCCTA
GAGCAAGAGAAGAAGGTGCGCATGGACCTGGAGCGAGCAAAGCGGAAACTGGAGGGCGAC
CTGAAGCTGACCCAGGAGAGCATCATGGACCTGGAAAATGATAAACTGCAGCTGGAAGAA
AAGCTTAAGAAGAAGGAGTTTGACATTAATCAGCAGAACAGTAAGATTGAGGATGAGCAG
GTGCTGGCCCTTCAACTACAGAAGAAACTGAAGGAAAACCAGGCACGCATCGAGGAGCTG
GAGGAGGAGCTGGAGGCCGAGCGCACCGCCAGGGCTAAGGTGGAGAAGCTGCGCTCAGAC
CTGTCTCGGGAGCTGGAGGAGATCAGCGAGCGGCTGGAAGAGGCCGGCGGGGCCACGTCC
GTGCAGATCGAGATGAACAAGAAGCGCGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTG
GAGGAGGCCACGCTGCAGCACGAGGCCACTGCCGCGGCCCTGCGCAAGAAGCACGCCGAC
AGCGTGGCCGAGCTGGGCGAGCAGATCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAG
AAGGAGAAGAGCGAGTTCAAGCTGGAGCTGGATGACGTCACCTCCAACATGGAGCAGATC
ATCAAGGCCAAGGCAAACCTGGAGAAAGTGTCTCGGACGCTGGAGGACCAGGCCAATGAG
TACCGCGTGAAGCTAGAAGAGGCCCAACGCTCCCTCAATGATTTCACCACCCAGCGAGCC
AAGCTGCAGACCGAGAATGGAGAGTTGGCCCGGCAGCTAGAGGAAAAGGAGGCGCTAATC
TCGCAGCTGACCCGGGGGAAGCTCTCTTATACCCAGCAAATGGAGGACCTCAAAAGGCAG
CTGGAGGAGGAGGGCAAGGCGAAGAACGCCCTGGCCCATGCACTGCAGTCGGCCCGGCAT
GACTGCGACCTGCTGCGGGAGCAGTACGAGGAGGAGACAGAGGCCAAGGCCGAGCTGCAG
CGCGTCCTGTCCAAGGCCAACTCGGAGGTGGCCCAGTGGAGGACCAAGTATGAGACGGAC
GCCATTCAGCGGACTGAGGAGCTCGAAGAGGCCAAAAAGAAGCTGGCCCAGCGGCTGCAG
GATGCCGAGGAGGCCGTGGAGGCTGTTAATGCCAAGTGCTCCTCACTGGAGAAGACCAAG
CACCGGCTACAGAATGAGATAGAGGACTTGATGGTGGACGTAGAGCGCTCCAATGCTGCT
GCTGCAGCCCTGGACAAGAAGCAGAGAAACTTTGACAAGATCCTGGCCGAGTGGAAGCAG
AAGTATGAGGAGTCGCAGTCTGAGCTGGAGTCCTCACAGAAGGAGGCTCGCTCCCTCAGC
ACAGAGCTCTTCAAGCTCAAGAACGCCTACGAGGAGTCCCTGGAGCACCTAGAGACCTTC
AAGCGGGAGAACAAGAACCTTCAGGAGGAAATCTCGGACCTTACTGAGCAGCTAGGAGAA
GGAGGAAAGAATGTGCATGAGCTGGAGAAGGTCCGCAAACAGCTGGAGGTGGAGAAGCTG
GAGCTGCAGTCAGCCCTGGAGGAGGCAGAGGCCTCCCTGGAGCACGAGGAGGGCAAGATC
CTCCGGGCCCAGCTAGAGTTCAACCAGATCAAGGCAGAGATCGAGCGGAAGCTGGCAGAG
AAGGACGAGGAGATGGAACAGGCCAAGCGCAACCACCAGCGGGTGGTGGACTCGCTGCAG
ACCTCCCTGGATGCAGAGACACGCAGCCGCAACGAGGTCCTGAGGGTGAAGAAGAAGATG
GAAGGAGACCTCAATGAGATGGAGATCCAGCTCAGCCACGCCAACCGCATGGCTGCCGAG
GCCCAGAAGCAAGTCAAGAGCCTCCAGAGCTTGCTGAAGGACACCCAGATCCAGCTGGAC
GATGCGGTCCGTGCCAACGACGACCTGAAGGAGAACATCGCCATCGTGGAGCGGCGCAAC
AACCTGCTGCAGGCTGAGCTGGAGGAGCTGCGTGCCGTGGTGGAGCAGACAGAGCGGTCC
CGGAAGCTGGCGGAGCAGGAGCTGATTGAGACCAGCGAGCGGGTGCAGCTGCTGCATTCC
CAGAACACCAGCCTCATCAACCAGAAGAAGAAGATGGAGTCGGATCTGACCCAGCTCCAG
TCGGAAGTGGAGGAGGCAGTGCAGGAGTGCAGAAACGCCGAGGAGAAGGCCAAGAAGGCC
ATCACGGATGCCGCCATGATGGCAGAGGAGCTGAAGAAGGAGCAGGACACCAGCGCCCAC
CTGGAGCGCATGAAGAAGAACATGGAGCAGACCATTAAGGACCTGCAGCACCGGCTGGAC
GAGGCCGAGCAGATCGCCCTCAAGGGAGGCAAGAAGCAGCTGCAGAAGCTGGAAGCGCGG
GTGCGGGAGCTGGAGGGTGAGCTGGAGGCCGAGCAGAAGCGCAACGCAGAGTCGGTGAAG
GGCATGAGGAAGAGCGAGCGGCGCATCAAGGAGCTCACCTACCAGACAGAGGAAGACAAA
AAGAACCTGCTGCGGCTACAGGACCTGGTGGACAAGCTGCAACTGAAGGTCAAGGCCTAC
AAGCGCCAGGCCGAGGAGGCGGAGGAGCAAGCCAACACCAACCTGTCCAAGTTCCGCAAG
GTGCAGCATGAGCTGGATGAGGCAGAGGAGCGGGCGGACATCGCTGAGTCCCAGGTCAAC
AAGCTTCGAGCCAAGAGCCGTGACATTGGTGCCAAGCAAAAAATGCACGATGAGGAGTTG
Protein sequence predicted based on nucleotide sequence
MTDAQMADFGAAAQYLRKSEKERLEAQTRPFDIRTECFVPDDKEEFVKAKILSREGGKVI AETENGKTVTVKEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLFNLKERYAAWMIYTYSGL FCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGES GAGKTVNTKRVIQYFASIAAIGDRGKKDNANANKGTLEDQIIQANPALEAFGNAKTVRND NSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLD MLLVTNNPYDYAFVSQGEVSVASIDDSEELMATDSAFDVLGFTSEEKAGVYKLTGAIMHY GNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQ VYYSIGALAKAVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCIN FTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECM FPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLEKNKD PLNETVVALYQKSSLKLMATLFSSYATADTGDSGKSKGGKKKGSSFQTVSALHRENLNKL MTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDF RQRYRILNPVAIPEGQFIDSRKGTEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRD ERLSRIITRMQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKP LLKSAETEKEMATMKEEFGRIKETLEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNL NDAEERCDQLIKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLE LTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQVEEDKVN SLSKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKLQLEE KLKKKEFDINQQNSKIEDEQVLALQLQKKLKENQARIEELEEELEAERTARAKVEKLRSD LSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHAD SVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANE YRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQ LEEEGKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETD AIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAA AAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETF KRENKNLQEEISDLTEQLGEGGKNVHELEKVRKQLEVEKLELQSALEEAEASLEHEEGKI LRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHQRVVDSLQTSLDAETRSRNEVLRVKKKM EGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRN NLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQ SEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLD EAEQIALKGGKKQLQKLEARVRELEGELEAEQKRNAESVKGMRKSERRIKELTYQTEEDK KNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVN KLRAKSRDIGAKQKMHDEEL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
