Gene:
Gene Symbol:
ITPR3
Gene Name:
inositol 1,4,5-trisphosphate receptor, type 3
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00946199
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 8016nts
Open reading frame: 1 to 8016
ATGAGTGAAATGTCCAGCTTTCTTCACATCGGGGACATCGTCTCCCTGTACGCCGAGGGC
TCCGTCAATGGCTTCATCAGCACTTTGGGGCTGGTGGATGACCGCTGTGTGGTGGAGCCC
GCGGCCGGGGACCTGGACAACCCCCCTAAGAAGTTCCGTGACTGCCTCTTCAAGGTGTGC
CCCATGAACCGCTACTCGGCCCAGAAGCAGTACTGGAAGGCCAAGCAGACTAAGCAGGAC
AAGGAGAAGATCGCTGATGTGGTGTTGCTGCAGAAGCTGCAGCATGCGGCGCAGATGGAG
CAGAAGCAAAATGACACGGAGAACAAGAAGGTGCATGGGGATGTCGTGAAGTATGGCAGT
GTGATCCAGCTCCTGCACATGAAGAGCAACAAGTACCTGACAGTGAACAAGCGGCTTCCG
GCCTTGCTGGAGAAGAACGCCATGCGGGTGACTCTGGATGCCACAGGCAACGAGGGTTCC
TGGCTCTTCATCCAGCCCTTCTGGAAGCTGCGGAGCAACGGGGACAACGTGGTCGTGGGG
GACAAGGTGATCCTGAATCCTGTCAATGCCGGGCAGCCTCTGCATGCCAGCAATTACGAG
CTCAGCGACAACGCCGGCTGCAAGGAGGTCAATTCTGTGAACTGCAACACCAGCTGGAAG
ATCAACCTGTTTATGCAGTTTCGGGACCACCTGGAGGAGGTGTTGAAAGGGGGAGACGTG
GTGCGGCTGTTCCATGCGGAGCAGGAGAAGTTCCTGACGTGTGACGAGTACAAGGGCAAG
CTGCAGGTGTTCCTGCGAACTACACTGCGCCAGTCTGCCACCTCGGCCACCAGCTCCAAT
GCTCTCTGGGAGGTGGAGGTGGTCCACCACGACCCCTGCCGTGGAGGAGCTGGGCACTGG
AATGGCTTGTACCGCTTCAAGCACCTGGCTACAGGCAACTACCTGGCTGCTGAGGAGAAC
CCCAGTTACAAAGGTGATGCCTCAGATCCCAAGGCAGCAGGAATGGGGGCACAGGGCCGC
ACAGGCCGCAGGAATGCTGGGGAGAAGATCAAGTACTGCCTGGTGGCTGTGCCTCATGGC
AATGACATCGCCTCTCTCTTTGAGCTGGACCCCACCACCTTGCAGAAAACCGACTCTTTC
GTGCCCCGGAACTCGTACGTCCGGCTGCGGCACCTCTGCACCAACACGTGGATTCAGAGC
ACCAATGTGCCCATTGACATCGAGGAGGAGCGGCCCATCCGGCTCATGCTGGGCACCTGC
CCCACCAAGGAGGACAAGGAGGCCTTTGCCATCGTGTCAGTGCCCGTGTCTGAGATCCGA
GACCTGGACTTTGCCAATGACGCCAGCTCCATGCTGGCCAGTGCCGTGGAGAAACTCAAC
GAGGGCTTCATCAGCCAGAATGACCGCAGGTTTGTCATCCAGCTGCTGGAAGACCTGGTG
TTCTTTGTCAGCGATGTCCCCAACAATGGGCAGAATGTCCTGGACATCATGGTCACTAAG
CCCAACCGGGAACGGCAGAAGCTGATGAGGGAGCAGAACATCCTCAAACAGGTCTTTGGC
ATTCTGAAGGCCCCGTTCCGTGAGAAGGGGGGTGAAGGTCCCCTGGTGCGGCTGGAGGAG
CTGTCAGACCAGAAGAACGCCCCCTACCAGCACATGTTCCGCCTGTGCTACCGCGTGTTG
CGGCATTCCCAGGAGGACTACCGCAAGAACCAGGAGCACATTGCCAAGCAGTTTGGGATG
ATGCAGTCCCAGATTGGCTACGACATCCTGGCCGAGGACACCATCACTGCCCTGCTGCAC
AACAACCGCAAGCTCCTGGAAAAGCACATCACCAAGACCGAGGTGGAGACCTTCGTCAGC
CTTGTGCGCAAGAACCGGGAGCCCAGGTTCCTGGACTACCTCTCTGACCTGTGTGTGTCC
AACCACATCGCCATCCCCGTCACCCAAGAGCTCATCTGCAAGTGTGTGCTGGACCCCAAG
AACAGTGACATTCTCATCCGGACCGAGCTTCGGCCCGTGAAGGAGATGGCCCAATCCCAC
GAGTACCTGAGCATCGAGTACTCAGAAGAGGAAGTGTGGCTCACGTGGACTGACAAGAAT
AACGAGCATCATGAGAAGAGTGTGAGGCAGCTGGCCCAGGAGGCGCGGGCCGGCAACGCC
CACGACGAGAATGTGCTCAGCTACTACAGGTACCAGCTGAAGCTCTTTGCCCGCATGTGC
TTGGACCGCCAGTACTTGGCCATCGACGAGATCTCCCAGCAGCTGGGCGTGGACCTGATT
TTCCTGTGCATGGCAGACGAGATGCTGCCCTTTGACCTGCGCGCCTCCTTCTGCCACCTG
ATGCTGCACGTGCACGTGGACCGTGACCCCCAGGAGCTGGTCACGCCGGTCAAGTTTGCC
CGTCTCTGGACTGAGATCCCCACAGCCATCACCATCAAGGACTATGATTCCAACCTCAAC
GCGTCCCGAGATGACAAGAAGAACAAGTTTGCCAACACCATGGAGTTCGTGGAGGACTAC
CTCAACAATGTAGTCAGCGAGGCCGTGCCCTTTGCCAACGAGGAGAAGAACAAGCTCACT
TTTGAGGTGGTCAGCCTGGCGCACAATCTCATCTACTTCGGCTTCTACAGCTTCAGCGAG
CTGCTGCGGCTCACTCGCACACTGCTGGGCATCATCGACTGTGTGCAGGGGCCCCCGGCC
ATGCTGCAGGCCTATGAGGACCCCGGTGGCAAGAATGTGCGGCGGTCCATCCAGGGCGTG
GGGCACATGATGTCCACCATGGTGCTGAGCCGCAAGCAGTCCGTCTTCAGTGCCCCCAGC
CTGTCTGCTGGGGCCAGTGCTGCTGAGCCGCTGGACAGAAGCAAGTTTGAGGAGAATGAG
GACATTGTGGTGATGGAGACCAAGCTGAAGATCCTGGAAATCCTTCAGTTCATCCTCAAT
GTCCGCCTGGATTACCGCATATCCTACCTGCTGTCTGTCTTCAAGAAGGAGTTTGTGGAG
GTGTTTCCCATGCAGGACAGTGGGGCTGATGGCACAGCCCCTGCCTTCGACTCTACCACT
GCCAACATGAACCTGGATCGCATCGGGGAGCAGGCGGAGGCCATGTTTGGAGTGGGGAAG
ACAAGCAGCATGCTGGAGGTGGATGACGAGGGCGGCCGCATGTTCCTGCGCGTGCTCATC
CACCTCACCATGCACGACTATGCGCCGCTGGTCTCGGGTGCCCTGCAGCTGCTCTTCAAG
CACTTCAGCCAGCGCCAGGAGGCCATGCACACCTTCAAGCAGGTTCAGCTGCTGATCTCA
GCGCAGGACGTGGAGAACTACAAGGTGATCAAGTCGGAGCTGGACCGGCTGCGGACCATG
GTGGAGAAGTCAGAGCTGTGGGTGGACAAGAAGGGCAGTGGCAAGGGTGAGGAGGTGGAG
GCAGGCGCCGCCAAGGACAAGAAAGAGCGTCCCACGGACGAGGAGGGCTTTCTGCACCCA
CCAGGGGAGAAAAGCAGTGAGAACTACCAGATCGTCAAGGGCATCCTGGAAAGGCTGAAC
AAGATGTGCGGGGTTGGGGAGCAAATGAGGAAGAAGCAGCAACGGCTGCTGAAGAACATG
GATGCCCACAAGGTCATGCTGGACCTGCTGCAGATCCCCTATGACAAGGGTGATGCCAAG
ATGATGGAGATCCTGCGCTACACGCACCAGTTCCTGCAGAAGTTCTGTGCAGGGAACCCC
GGCAACCAGGCCCTGCTGCACAAACACCTGCACCTCTTCCTCACGCCAGGGCTCCTGGAG
GCAGAGACCATGCAGCACATCTTCCTGAACAACTATCAGCTCTGCTCCGAGATCAGCGAG
CCTGTGTTGCAGCACTTCGTGCACCTGCTGGCCACGCACGGGCGCCATGTGCAGTACCTG
GACTTCCTGCACACCGTCATTAAGGCCGAGGGCAAGTACGTCAAGAAGTGCCAGGACATG
ATCATGACTGAGCTGACCAATGCAGGTGACGATGTGGTCGTGTTCTACAATGATAAGGCA
TCGCTGGCCCACCTGCTGGACATGATGAAGGCCGCCCGCGACGGCGTGGAGGACCACAGC
CCCCTCATGTACCACATTTCCCTGGTGGACCTGCTGGCCGCCTGTGCCGAGGGCAAAAAC
GTCTACACTGAGATCAAGTGCACCTCCCTGCTGCCGCTGGAGGACGTGGTGTCTGTGGTG
ACGCATGAGGACTGCATCACTGAGGTGAAAATGGCCTATGTGAACTTCGTGAACCACTGC
TACGTGGACACGGAGGTGGAGATGAAGGAGATCTACACCAGCAACCACATCTGGACGCTC
TTTGAGAACTTCACCCTGGACATGGCTCGGGTCTGCAGCAAGCGTGAGAAGCGCGTGGCT
GACCCCACCTTGGAGAAGTACGTGCTGAGCGTTGTGCTGGACACCATCAACGCCTTCTTC
AGCTCCCCATTCTCTGAGAACAGCACTTCCCTGCAGACACACCAGACGATTGTGGTGCAG
CTGCTGCAGTCTACCACACGCCTCCTCGAGTGTCCGTGGCTACAGCAGCAGCACAAGGGC
TCCGTGGAGGCCTGCATCCGGACCCTCGCCATGGTGGCCAAGGGCCGGGCCATCTTGCTG
CCCATGGACCTGGATGCCCACATCAGCTCGATGCTCAGCAGTGGAGCCAGCTGTGCAGCT
GCCGCCCAGCGGAACGCCTCCAGCTACAAGGCAACCACGCGGGCCTTCCCCCGCGTCACC
CCCACCGCCAACCAGTGGGACTACAAGAACATCATTGAGAAGCTGCAGGACATCATCACA
GCCCTGGAGGAGCGGCTGAAGCCCCTGGTACAGGCTGAGCTGTCCGTGCTGGTGGATGTC
CTGCACTGGCCTGAGCTGCTCTTCCTGGAGGGCAGTGAGGCCTACCAGCGCTGCGAGAGT
GGGGGCTTCCTGTCCAAGCTGATCCAGCACACCAAGGACCTCATGGAGTCGGAGGAGAAG
CTGTGCATCAAGGTGCTGCGGACCCTGCAGCAGATGCTGCTCAAGAAGACCAAGTACGGG
GACCGGGGCAACCAGCTGCGCAAGATGCTGCTGCAAAACTACCTCCAGAACCGGAAGTCC
ACCTCGCGGGGGGACCTTCCCGACCCCATAGGCACTGGCCTGGACCCAGACTGGTCGGCA
ATCGCAGCCACCCAGTGCCGGCTGGACAAGGAGGGGGCCACCAAGTTGGTATGCGACCTC
ATCACCAGCACCAAGAACGAGAAGATCTTCCAGGAGAGCATCGGCCTGGCCATCCACCTG
CTGGATGGTGGCAACACAGAGATCCAGAAATCCTTCCACAACCTGATGATGAGTGACAAG
AAGTCAGAGCGCTTCTTCAAGGTGCTGCACGACCGCATGAAGCGGGCCCAGCAGGAGACC
AAGTCCACGGTGGCAGTCAACATGAATGACCTGGGCAGCCAGCCACATGAGGACCGCGAG
CCAGTCGACCCCACCACCAAAGGCCGCGTGGCCTCCTTCTCGATACCTGGCTCCTCATCC
CGCTACTCGCTGGGCCCCAGCCTGCGCCGGGGGCACGAGGTGAGCGAACGTGTGCAGAGC
AGTGAGATGGGCACATCCGTGCTCATCATGCAGCCCATCCTGCGCTTTCTGCAGCTGCTG
TGTGAGAACCACAACCGGGACCTGCAGAACTTCCTGCGCTGTCAGAACAACAAAACCAAC
TACAACTTGGTATGCGAGACGCTGCAGTTCCTGGACATCATGTGCGGCAGCACCACGGGC
GGCCTGGGGCTGCTGGGGCTCTACATCAATGAGGACAACGTGGGCCTCGTCATCCAGACC
TTGGAGACCCTCACTGAGTACTGCCAGGGCCCCTGCCATGAGAACCAGACTTGCATTGTG
ACTCACGAGTCCAATGGCATAGACATCATCACCGCACTGATCCTCAATGACATCAGCCCC
CTGTGCAAGTACCGCATGGATCTGGTGCTGCAGCTCAAGGACAATGCCTCCAAGCTGCTC
CTGGCTCTGATGGAGAGCCGGCATGACAGTGAAAATGCTGAGCGAATCCTCATCAGCCTG
CGGCCCCAGGAGCTGGTGGACGTCATCAAGAAGGCCTACCTGCAGGAGGAAGAGCGTGAG
AACTCGGAGGTGAGCCCACGTGAAGTGGGCCATAACATCTATATCCTGGCGCTGCAGCTC
TCCAGGCACAATAAACAGCTGCAGCACCTGCTGAAGCCGGTGAAGCGCATTCAAGAGGAG
GAGGCCGAGGGTATCTCTTCCATGCTCAGCCTCAACAACAAGCAGCTGTCACAGATGCTC
AAGTCCTCAGCGCCAGCACAGGAGGAGGAGGAAGACCCCCTGGCCTACTATGAGAACCAC
ACGTCCCAGATCGAGATTGTGCGGCAGGACCGCAGCATGGAGCAGATCGTGTTCCCAGTG
CCCGGCATCTGCCAGTTCCTGACGGAGGAAACCAAGCACCGGCTCTTCACCACTACTGAG
CAGGACGAGCAGGGCAGCAAAGTGAGCGACTTCTTCGACCAGTCCTCCTTCCTGCACAAC
GAGATGGAGTGGCAGCGCAAGCTCCGCAGCATGCCGCTGATCTACTGGTTCTCCCGCCGC
ATGACCCTGTGGGGCAGCATCTCCTTCAACCTGGCCGTGTTTATCAACATCATCATTGCC
TTCTTCTACCCTTACATGGAGGGCGCGTCCACAGGCGTGCTGGACTCCCCTCTCATCTCA
TTGCTCTTCTGGATCCTCATCTGCTTCTCCATCGCGGCCCTGTTCACCAAGCGCTACAGC
ATCCGCCCCCTCATCGTGGCGCTCATCCTGCGCTCCATCTACTATCTGGGCATCGGGCCC
ACACTCAACATCCTGGGTGCCCTCAATCTGACCAACAAGATCGTGTTTGTGGTGAGCTTC
GTGGGCAACCGTGGCACCTTCATCCGGGGCTATAAGGCCATGGTCATGGACATGGAATTC
CTCTACCACGTGGGCTACATCCTGACCAGTGTCCTGGGCCTCTTTGCTCATGAGCTGTTC
TACAGCATCCTGCTCTTTGACCTCATCTACCGCGAGGAGACGCTGTTCAACGTCATCAAG
AGTGTGACCCGCAATGGCCGCTCCATCCTGCTGACAGCCCTGCTGGCCCTCATCCTGGTC
TACCTCTTCTCCATCGTCGGCTTCCTCTTCCTCAAGGATGACTTCATTCTCGAGGTCGAC
CGGCTGCCCAACAACCACTCCACAGCCAGCCCCCTGGGGATGCCACATGGAGCTGCTGCA
TTTGTGGACACCTGCAGTGGGGACAAGATGGACTGTGTCTCAGGGCTCTCGGTGCCTGAG
GTCCTGGAAGAGGACAGGGAGCTGGACAGCACAGAGCGGGCCTGTGACACTCTGTTGATG
TGCATCGTCACTGTCATGAACCATGGGCTACGCAACGGTGGTGGCGTGGGCGACATTCTC
CGCAAGCCCTCCAAAGATGAGTCTCTCTTCCCAGCCCGAGTGGTCTATGACCTCCTGTTC
TTCTTCATCGTCATCATCATTGTGCTGAACCTCATCTTTGGGGTAATCATCGACACCTTC
GCTGACCTGCGTAGTGAGAAGCAGAAGAAGGAGGAGATTCTTAAGACGACATGCTTCATC
TGTGGTCTGGAGAGGGACAAGTTTGATAACAAGACAGTGTCATTTGAGGAACACATCAAG
CTGGAGCACAACATGTGGAACTACTTGTACTTCATTGTGCTGGTCCGCGTGAAGAACAAG
ACCGACTACACGGGCCCTGAGAGCTACGTGGCCCAGATGATCAAGAACAAGAACCTGGAC
TGGTTCCCCCGGATGCGGGCCATGTCCCTTGTCAGCAATGAGGGCGAGGGGGAGCAGAAT
GAGATTCGGATTCTCCAGGACAAGCTCAACTCCACCATGAAGCTGGTGTCCCACCTCACT
GCCCAGCTCAACGAGCTCAAGGAGCAGATGACGGAGCAGCGGAAACGCAGGCAACGCCTA
GGCTTTGTGGATGTCCAGAACTGCATTAGCCGCTTG
Protein sequence predicted based on nucleotide sequence
MSEMSSFLHIGDIVSLYAEGSVNGFISTLGLVDDRCVVEPAAGDLDNPPKKFRDCLFKVC PMNRYSAQKQYWKAKQTKQDKEKIADVVLLQKLQHAAQMEQKQNDTENKKVHGDVVKYGS VIQLLHMKSNKYLTVNKRLPALLEKNAMRVTLDATGNEGSWLFIQPFWKLRSNGDNVVVG DKVILNPVNAGQPLHASNYELSDNAGCKEVNSVNCNTSWKINLFMQFRDHLEEVLKGGDV VRLFHAEQEKFLTCDEYKGKLQVFLRTTLRQSATSATSSNALWEVEVVHHDPCRGGAGHW NGLYRFKHLATGNYLAAEENPSYKGDASDPKAAGMGAQGRTGRRNAGEKIKYCLVAVPHG NDIASLFELDPTTLQKTDSFVPRNSYVRLRHLCTNTWIQSTNVPIDIEEERPIRLMLGTC PTKEDKEAFAIVSVPVSEIRDLDFANDASSMLASAVEKLNEGFISQNDRRFVIQLLEDLV FFVSDVPNNGQNVLDIMVTKPNRERQKLMREQNILKQVFGILKAPFREKGGEGPLVRLEE LSDQKNAPYQHMFRLCYRVLRHSQEDYRKNQEHIAKQFGMMQSQIGYDILAEDTITALLH NNRKLLEKHITKTEVETFVSLVRKNREPRFLDYLSDLCVSNHIAIPVTQELICKCVLDPK NSDILIRTELRPVKEMAQSHEYLSIEYSEEEVWLTWTDKNNEHHEKSVRQLAQEARAGNA HDENVLSYYRYQLKLFARMCLDRQYLAIDEISQQLGVDLIFLCMADEMLPFDLRASFCHL MLHVHVDRDPQELVTPVKFARLWTEIPTAITIKDYDSNLNASRDDKKNKFANTMEFVEDY LNNVVSEAVPFANEEKNKLTFEVVSLAHNLIYFGFYSFSELLRLTRTLLGIIDCVQGPPA MLQAYEDPGGKNVRRSIQGVGHMMSTMVLSRKQSVFSAPSLSAGASAAEPLDRSKFEENE DIVVMETKLKILEILQFILNVRLDYRISYLLSVFKKEFVEVFPMQDSGADGTAPAFDSTT ANMNLDRIGEQAEAMFGVGKTSSMLEVDDEGGRMFLRVLIHLTMHDYAPLVSGALQLLFK HFSQRQEAMHTFKQVQLLISAQDVENYKVIKSELDRLRTMVEKSELWVDKKGSGKGEEVE AGAAKDKKERPTDEEGFLHPPGEKSSENYQIVKGILERLNKMCGVGEQMRKKQQRLLKNM DAHKVMLDLLQIPYDKGDAKMMEILRYTHQFLQKFCAGNPGNQALLHKHLHLFLTPGLLE AETMQHIFLNNYQLCSEISEPVLQHFVHLLATHGRHVQYLDFLHTVIKAEGKYVKKCQDM IMTELTNAGDDVVVFYNDKASLAHLLDMMKAARDGVEDHSPLMYHISLVDLLAACAEGKN VYTEIKCTSLLPLEDVVSVVTHEDCITEVKMAYVNFVNHCYVDTEVEMKEIYTSNHIWTL FENFTLDMARVCSKREKRVADPTLEKYVLSVVLDTINAFFSSPFSENSTSLQTHQTIVVQ LLQSTTRLLECPWLQQQHKGSVEACIRTLAMVAKGRAILLPMDLDAHISSMLSSGASCAA AAQRNASSYKATTRAFPRVTPTANQWDYKNIIEKLQDIITALEERLKPLVQAELSVLVDV LHWPELLFLEGSEAYQRCESGGFLSKLIQHTKDLMESEEKLCIKVLRTLQQMLLKKTKYG DRGNQLRKMLLQNYLQNRKSTSRGDLPDPIGTGLDPDWSAIAATQCRLDKEGATKLVCDL ITSTKNEKIFQESIGLAIHLLDGGNTEIQKSFHNLMMSDKKSERFFKVLHDRMKRAQQET KSTVAVNMNDLGSQPHEDREPVDPTTKGRVASFSIPGSSSRYSLGPSLRRGHEVSERVQS SEMGTSVLIMQPILRFLQLLCENHNRDLQNFLRCQNNKTNYNLVCETLQFLDIMCGSTTG GLGLLGLYINEDNVGLVIQTLETLTEYCQGPCHENQTCIVTHESNGIDIITALILNDISP LCKYRMDLVLQLKDNASKLLLALMESRHDSENAERILISLRPQELVDVIKKAYLQEEERE NSEVSPREVGHNIYILALQLSRHNKQLQHLLKPVKRIQEEEAEGISSMLSLNNKQLSQML KSSAPAQEEEEDPLAYYENHTSQIEIVRQDRSMEQIVFPVPGICQFLTEETKHRLFTTTE QDEQGSKVSDFFDQSSFLHNEMEWQRKLRSMPLIYWFSRRMTLWGSISFNLAVFINIIIA FFYPYMEGASTGVLDSPLISLLFWILICFSIAALFTKRYSIRPLIVALILRSIYYLGIGP TLNILGALNLTNKIVFVVSFVGNRGTFIRGYKAMVMDMEFLYHVGYILTSVLGLFAHELF YSILLFDLIYREETLFNVIKSVTRNGRSILLTALLALILVYLFSIVGFLFLKDDFILEVD RLPNNHSTASPLGMPHGAAAFVDTCSGDKMDCVSGLSVPEVLEEDRELDSTERACDTLLM CIVTVMNHGLRNGGGVGDILRKPSKDESLFPARVVYDLLFFFIVIIIVLNLIFGVIIDTF ADLRSEKQKKEEILKTTCFICGLERDKFDNKTVSFEEHIKLEHNMWNYLYFIVLVRVKNK TDYTGPESYVAQMIKNKNLDWFPRMRAMSLVSNEGEGEQNEIRILQDKLNSTMKLVSHLT AQLNELKEQMTEQRKRRQRLGFVDVQNCISRL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
