Gene:
Gene Symbol:
LRP4
Gene Name:
low density lipoprotein receptor-related protein 4
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00946200
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 5718nts
Open reading frame: 1 to 5718
ATGAGGCGGCAGTGGGGCGCGCTGCTGCTTGGCGCCCTGCTCTGCGCACACGGCCTGGCC
AGCAGCCCCGAGTGTGCTTGTGGTCGGAGCCACTTCACATGTGCAGTGAGTGCTCTTGGA
GAGTGTACCTGCATCCCTGCCCAGTGGCAGTGTGATGGAGACAATGACTGCGGGGACCAC
AGCGATGAGGATGGATGTATACTACCTACCTGTTCCCCTCTTGACTTTCACTGTGACAAT
GGCAAGTGCATCCGCCGCTCCTGGGTGTGTGACGGGGACAACGACTGTGAGGATGACTCG
GATGAGCAGGACTGTCCCCCCCGGGAGTGTGAGGAGGACGAGTTTCCCTGCCAGAATGGC
TACTGCATCCGGAGTCTGTGGCACTGCGATGGTGACAATGACTGTGGCGACAACAGCGAT
GAGCAGTGTGACATGCGCAAGTGCTCCGACAAGGAGTTCCGCTGTAGTGACGGAAGCTGC
ATTGCTGAGCATTGGTACTGCGACGGTGACACCGACTGCAAAGATGGCTCCGATGAGGAG
AACTGTCCCTCAGCAGTGCCAGCGCCCCCCTGCAACCTGGAGGAGTTCCAGTGTGCCTAT
GGACGCTGCATCCTCGACATCTACCACTGCGATGGCGACGATGACTGTGGAGACTGGTCA
GACGAGTCTGACTGCTCCTCCCACCAGCCCTGCCGCTCTGGGGAGTTCATGTGTGACAGT
GGCCTGTGCATCAATGCAGGCTGGCGCTGCGATGGTGACGCGGACTGTGATGACCAGTCT
GATGAGCGCAACTGCACCACCTCCATGTGTACGGCAGAACAGTTCCGCTGTCACTCAGGC
CGCTGTGTCCGCCTGTCCTGGCGCTGTGATGGGGAGGACGACTGTGCAGACAACAGCGAT
GAAGAGAACTGTGAGAATACAGGAAGCCCCCAATGTGCCTTGGACCAGTTCCTGTGTTGG
AATGGGCGCTGCATTGGGCAGAGGAAGCTGTGCAACGGGGTCAACGACTGTGGTGACAAC
AGCGACGAAAGCCCACAGCAGAATTGCCGGCCCCGGACGGGTGAGGAGAACTGCAATGTT
AACAACGGTGGCTGTGCCCAGAAGTGCCAGATGGTGCGGGGGGCAGTGCAGTGTACCTGC
CACACAGGCTACCGGCTCACAGAGGATGGGCACACGTGCCAAGATGTGAATGAATGTGCC
GAGGAGGGGTATTGCAGCCAGGGCTGCACCAACAGCGAAGGGGCTTTCCAATGCTGGTGT
GAAACAGGCTATGAACTACGGCCCGACCGGCGCAGCTGCAAGGCTCTGGGGCCAGAGCCT
GTGCTGCTGTTCGCCAATCGCATCGACATCCGGCAGGTGCTGCCACACCGCTCTGAGTAC
ACACTGCTGCTTAACAACCTGGAGAATGCCATTGCCCTTGATTTCCACCACCGCCGCGAG
CTTGTCTTCTGGTCAGATGTCACCCTGGACCGGATCCTCCGTGCCAACCTCAACGGCAGC
AACGTGGAGGAGGTTGTGTCTACTGGGCTGGAGAGCCCAGGGGGCCTGGCTGTGGATTGG
GTCCATGACAAACTCTACTGGACCGACTCAGGCACCTCGAGGATTGAGGTGGCCAATCTG
GATGGGGCCCACCGGAAAGTGTTGCTGTGGCAGAACCTGGAGAAGCCCCGGGCCATTGCC
TTGCATCCCATGGAGGGTACCATTTACTGGACAGACTGGGGCAACACCCCCCGTATTGAG
GCCTCCAGCATGGATGGCTCTGGACGCCGCATCATTGCCGATACCCATCTCTTCTGGCCC
AATGGCCTCACCATCGACTATGCCGGGCGCCGTATGTACTGGGTGGATGCTAAGCACCAT
GTCATCGAGAGGGCCAATCTGGATGGGAGTCACCGTAAGGCTGTCATTAGCCAGGGCCTC
CCGCATCCCTTCGCCATCACAGTGTTTGAAGACAGCCTGTACTGGACAGACTGGCACACC
AAGAGCATCAATAGCGCTAACAAATTTACGGGGAAGAACCAGGAAATCATTCGCAACAAA
CTCCACTTCCCTATGGACATCCACACCTTGCACCCCCAGCGCCAACCTGCAGGGAAAAAC
CGCTGTGGGGACAACAACGGAGGCTGCACGCACCTGTGTCTGCCCAGTGGCCAGAACTAC
ACCTGTGCCTGCCCCACTGGCTTCCGCAAGATCAGCAGCCACGCCTGTGCCCAGAGTCTT
GACAAGTTCCTGCTTTTTGCCCGAAGGATGGACATCCGTCGAATCAGCTTTGACACAGAG
GACCTGTCTGATGATGTCATCCCACTGGCTGACGTGCGCAGTGCTGTGGCCCTTGACTGG
GACTCCCGGGATGACCACGTGTACTGGACAGATGTCAGCACTGATACCATCAGCAGGGCC
AAGTGGGATGGAACAGGACAGGAGGTGGTAGTGGATACCAGTTTGGAGAGCCCAGCTGGC
CTGGCCATTGATTGGGTCACCAACAAACTGTACTGGACAGATGCAGGTACAGACCGGATT
GAAGTAGCCAACACAGATGGCAGCATGAGAACAGTACTCATCTGGGAGAACCTTGATCGT
CCTCGGGACATCGTGGTGGAACCCATGGGCGGGTACATGTATTGGACTGACTGGGGTGCG
AGCCCCAAGATTGAACGAGCTGGCATGGATGCCTCAGGCCGCCAAGTCATTATCTCTTCT
AATCTGACCTGGCCTAATGGGTTAGCTATTGATTATGGGTCCCAGCGTCTATACTGGGCT
GACGCCGGCATGAAGACAATTGAATTTGCTGGACTGGATGGCAGTAAGAGGAAGGTGCTG
ATTGGAAGCCAGCTCCCCCACCCATTTGGGCTGACCCTCTATGGAGAGCGCATCTATTGG
ACTGACTGGCAGACCAAGAGCATACAGAGCGCTGACCGGCTGACAGGGCTGGACCGGGAG
ACTCTGCAGGAGAACCTGGAAAACCTAATGGACATCCATGTCTTCCACCGCCGCCGGCCC
CCAGTGTCTACACCATGTGCTATGGAGAATGGCGGCTGTAGCCACCTGTGTCTTAGGTCC
CCAAATCCAAGCGGATTCAGCTGTACCTGCCCCACAGGCATCAACCTGCTGTCTGATGGC
AAGACCTGCTCACCAGGCATGAACAGTTTCCTCATCTTCGCCAGGAGGATAGACATTCGC
ATGGTCTCCCTGGACATCCCTTATTTTGCTGATGTGGTGGTACCAATCAACATTACCATG
AAGAACACCATTGCCATTGGAGTAGACCCCCAGGAAGGAAAGGTGTACTGGTCTGACAGC
ACACTGCACAGGATCAGTCGTGCCAATCTGGATGGCTCACAGCATGAGGACATCATCACC
ACAGGGCTACAGACCACAGATGGGCTCGCGGTTGATGCCATTGGCCGGAAAGTATACTGG
ACAGACACGGGAACAAACCGGATTGAAGTGGGCAACCTGGACGGGTCCATGCGGAAAGTG
TTGGTGTGGCAGAACCTTGACAGTCCCCGGGCCATCGTACTGTACCATGAGATGGGGTTT
ATGTACTGGACAGACTGGGGGGAGAATGCCAAGTTAGAGCGGTCCGGAATGGATGGCTCA
GACCGCGCGGTGCTCATCAACAACAACCTAGGATGGCCCAATGGACTGACTGTGGACAAG
GCCAGCTCCCAACTGCTATGGGCCGATGCCCACACCGAGCGAATTGAGGCTGCTGACCTG
AATGGTGCCAATCGGCATACATTGGTGTCACCGGTGCAGCACCCATATGGCCTCACCCTG
CTCGACTCCTATATCTACTGGACTGACTGGCAGACTCGGAGCATCCACCGTGCTGACAAG
GGTACTGGCAGCAATGTCATCCTCGTGAGGTCCAACCTGCCAGGCCTCATGGACATGCAG
GCTGTGGACCGGGCACAGCCACTAGGTTTTAACAAGTGCGGCTCGAGAAATGGCGGCTGC
TCCCACCTCTGCTTGCCTCGGCCTTCTGGCTTCTCCTGTGCCTGCCCCACTGGCATCCAG
CTGAAGGGAGATGGGAAGACCTGTGATCCCTCTCCTGAGACCTACCTGCTCTTCTCCAGC
CGTGGCTCCATCCGGCGTATCTCACTGGACACCAGTGACCACACCGATGTGCATGTCCCT
GTTCCTGAGCTCAACAATGTCATCTCCCTGGACTATGACAGCGTGGATGGAAAGGTCTAT
TACACAGATGTGTTCCTGGATGTTATCAGGCGAGCAGACCTGAACGGCAGCAACATGGAG
ACAGTGATCGGGCGAGGGCTGAAGACCACTGACGGGCTGGCAGTGGACTGGGTGGCCAGG
AACCTGTACTGGACAGACACAGGTCGAAATACCATTGAGGCGTCCAGGCTGGATGGTTCC
TGCCGCAAAGTACTGATCAACAATAGCCTGGATGAGCCCCGGGCCATTGCTGTTTTCCCC
AGGAAGGGGTACCTCTTCTGGACAGACTGGGGCCACATTGCCAAGATCGAACGGGCAAAC
TTGGATGGTTCTGAGCGGAAGGTCCTCATCAACACAGACCTGGGTTGGCCCAATGGCCTT
ACCCTGGACTATGATACCCGCAGGATCTACTGGGTGGATGCGCATCTGGACCGGATCGAG
AGTGCTGACCTCAATGGGAAACTGCGGCAGGTCTTGGTCAGCCATGTGTCCCACCCCTTT
GCCCTCACACAGCAAGACAGGTGGATCTACTGGACAGACTGGCAGACCAAGTCAATCCAG
CGTGTTGACAAATACTCAGGCCGGAACAAGGAGACAGTGCTGGCAAATGTGGAAGGACTC
ATGGATATCATCGTGGTTTCCCCTCAGCGGCAGACAGGGACCAATGCCTGTGGTGTGAAC
AATGGTGGCTGCACCCACCTCTGCTTTGCCAGAGCCTCGGACTTCGTATGTGCCTGTCCT
GACGAACCTGATAGCCGGCCCTGCTCCCTTGTGCCTGGCCTGGTACCACCAGCTCCTAGG
GCTACTGGCATGAGTGAAAAGAGCCCAGTGCTACCCAACACACCACCTACCACCTTGTAT
TCTTCAACCACCCGGACCCGCACGTCTCTGGAGGAGGTGGAAGGAAGATGCTCTGAAAGG
GATGCCAGGCTGGGCCTCTGTGCACGTTCCAATGACGCTGTTCCTGCTGCTCCAGGGGAA
GGACTTCATATCAGCTACGCCATTGGTGGACTCCTCAGTATTCTGCTGATTTTGGTGGTG
ATTGCAGCTTTGATGCTGTACAGACACAAAAAATCCAAGTTCACTGATCCTGGAATGGGG
AACCTCACCTACAGCAACCCCTCCTACCGAACATCCACACAGGAAGTGAAGATTGAAGCA
ATCCCCAAACCAGCCATGTACAACCAGCTGTGCTATAAGAAAGAGGGAGGGCCTGACCAT
AACTACACCAAGGAGAAGATCAAGATCGTAGAGGGAATCTGCCTCCTGTCTGGGGATGAT
GCTGAGTGGGATGACCTCAAGCAACTGCGAAGCTCACGGGGGGGCCTCCTCCGGGATCAT
GTATGCATGAAGACAGACACGGTGTCCATCCAGGCCAGCTCTGGCTCCCTGGATGACACA
GAGACGGAGCAGCTGTTACAGGAAGAGCAGTCTGAGTGTAGCAGCGTCCATACTGCAGCC
ACTCCAGAAAGACGAGGCTCTCTGCCAGACACGGGCTGGAAACATGAACGCAAGCTCTCC
TCAGAGAGCCAGGTCTTG
Protein sequence predicted based on nucleotide sequence
MRRQWGALLLGALLCAHGLASSPECACGRSHFTCAVSALGECTCIPAQWQCDGDNDCGDH SDEDGCILPTCSPLDFHCDNGKCIRRSWVCDGDNDCEDDSDEQDCPPRECEEDEFPCQNG YCIRSLWHCDGDNDCGDNSDEQCDMRKCSDKEFRCSDGSCIAEHWYCDGDTDCKDGSDEE NCPSAVPAPPCNLEEFQCAYGRCILDIYHCDGDDDCGDWSDESDCSSHQPCRSGEFMCDS GLCINAGWRCDGDADCDDQSDERNCTTSMCTAEQFRCHSGRCVRLSWRCDGEDDCADNSD EENCENTGSPQCALDQFLCWNGRCIGQRKLCNGVNDCGDNSDESPQQNCRPRTGEENCNV NNGGCAQKCQMVRGAVQCTCHTGYRLTEDGHTCQDVNECAEEGYCSQGCTNSEGAFQCWC ETGYELRPDRRSCKALGPEPVLLFANRIDIRQVLPHRSEYTLLLNNLENAIALDFHHRRE LVFWSDVTLDRILRANLNGSNVEEVVSTGLESPGGLAVDWVHDKLYWTDSGTSRIEVANL DGAHRKVLLWQNLEKPRAIALHPMEGTIYWTDWGNTPRIEASSMDGSGRRIIADTHLFWP NGLTIDYAGRRMYWVDAKHHVIERANLDGSHRKAVISQGLPHPFAITVFEDSLYWTDWHT KSINSANKFTGKNQEIIRNKLHFPMDIHTLHPQRQPAGKNRCGDNNGGCTHLCLPSGQNY TCACPTGFRKISSHACAQSLDKFLLFARRMDIRRISFDTEDLSDDVIPLADVRSAVALDW DSRDDHVYWTDVSTDTISRAKWDGTGQEVVVDTSLESPAGLAIDWVTNKLYWTDAGTDRI EVANTDGSMRTVLIWENLDRPRDIVVEPMGGYMYWTDWGASPKIERAGMDASGRQVIISS NLTWPNGLAIDYGSQRLYWADAGMKTIEFAGLDGSKRKVLIGSQLPHPFGLTLYGERIYW TDWQTKSIQSADRLTGLDRETLQENLENLMDIHVFHRRRPPVSTPCAMENGGCSHLCLRS PNPSGFSCTCPTGINLLSDGKTCSPGMNSFLIFARRIDIRMVSLDIPYFADVVVPINITM KNTIAIGVDPQEGKVYWSDSTLHRISRANLDGSQHEDIITTGLQTTDGLAVDAIGRKVYW TDTGTNRIEVGNLDGSMRKVLVWQNLDSPRAIVLYHEMGFMYWTDWGENAKLERSGMDGS DRAVLINNNLGWPNGLTVDKASSQLLWADAHTERIEAADLNGANRHTLVSPVQHPYGLTL LDSYIYWTDWQTRSIHRADKGTGSNVILVRSNLPGLMDMQAVDRAQPLGFNKCGSRNGGC SHLCLPRPSGFSCACPTGIQLKGDGKTCDPSPETYLLFSSRGSIRRISLDTSDHTDVHVP VPELNNVISLDYDSVDGKVYYTDVFLDVIRRADLNGSNMETVIGRGLKTTDGLAVDWVAR NLYWTDTGRNTIEASRLDGSCRKVLINNSLDEPRAIAVFPRKGYLFWTDWGHIAKIERAN LDGSERKVLINTDLGWPNGLTLDYDTRRIYWVDAHLDRIESADLNGKLRQVLVSHVSHPF ALTQQDRWIYWTDWQTKSIQRVDKYSGRNKETVLANVEGLMDIIVVSPQRQTGTNACGVN NGGCTHLCFARASDFVCACPDEPDSRPCSLVPGLVPPAPRATGMSEKSPVLPNTPPTTLY SSTTRTRTSLEEVEGRCSERDARLGLCARSNDAVPAAPGEGLHISYAIGGLLSILLILVV IAALMLYRHKKSKFTDPGMGNLTYSNPSYRTSTQEVKIEAIPKPAMYNQLCYKKEGGPDH NYTKEKIKIVEGICLLSGDDAEWDDLKQLRSSRGGLLRDHVCMKTDTVSIQASSGSLDDT ETEQLLQEEQSECSSVHTAATPERRGSLPDTGWKHERKLSSESQVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
