Gene:
Gene Symbol:
IQSEC1
Gene Name:
IQ motif and Sec7 domain 1
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00946219
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3345nts
Open reading frame: 1 to 3345
ATGGCTTGCAGAAGACGCTATTTCGTCGAGGGCGAGGCCCCCAGCAGTGAGACTGGCACA
TCCCTGGACAGCCCCTCAGCCTACCCCCAGGGCCCCTTGGTGCCCGGTTCCAGCCTGAGC
CCGGATCACTACGAGCACACGTCAGTGGGAGCCTATGGGCTGTACTCGGGGCCGCCGGGG
CAACAGCAGCGCACGCGGAGGCCCAAGCTGCAGCACTCGACCTCCATCCTGCGCAAGCAG
GCTGAGGAGGAGGCCATCAAGCGCTCACGCTCACTCTCCGAGAGCTATGAGCTCTCCTCG
GACCTGCAGGACAAGCAGGTGGAGATGCTAGAACGAAAGTATGGGGGGCGCCTGGTAACC
CGCCATGCGGCCCGCACCATCCAGACGGCGTTTCGCCAGTACCAGATGAACAAGAACTTC
GAGCGCTTGCGCAGCTCCATGTCAGAGAACCGCATGTCACGCCGGATTGTGCTGTCCAAC
ATGAGGATGCAGTTCTCCTTTGAGGGGCCTGAGAAAGTGCACAGCTCCTACTTCGAGGGG
AAGCAGGTCTCAGTGACTAACGACGGCTCCCAGCTGGGAGCCCTGGTGTCCCCTGAGTGT
GGTGACCTCAGCGAGCCCACCACCCTCAAGTCTCCGGCCCCCTCCAGTGACTTTGCGGAC
GCCATCACCGAGCTGGAGGACGCCTTCTCTAGGCAAGTGAAATCACTGGCCGAGTCCATC
GACGATGCCCTCAACTGCCGCAGCCTGCACACTGAGGAGGCACCGGCCCTGGATGCGGCG
CGGGCCCGGGACACCGAACCCCAGACAGCCCTGCACGGCATGGACCACCGCAAACTGGAC
GAGATGACGGCCTCGTACAGTGATGTCACCCTGTACATCGATGAGGAGGAGCTGTCGCCC
CCTCTGCCCCTCTCGCAGGCAGGGGACCGGCCGTCCAGCACCGAGTCGGACCTGCGGCTA
CGGGCTGGGGGCGCAGCCCCAGACTACTGGGCCCTGGCCCACAAAGAGGACAAGGCTGAC
ACGGACACGAGCTGCCGGAGCACGCCGTCGCTGGAGCGGCAGGAGCAGCGGCTGCGGGTG
GAGCATCTGCCGCTGCTCACCATCGAGCCACCCAGCGACAGCTCTGTGGACCTTAGTGAC
CGCTCGGAGCGGGGGTCACTCAAGAGGCAGAGTGCTTACGAGCGCAGCCTTGGCGGGCAG
CAGGGCAGTCCCAAGCATGGTCCCCACAGCGGCGCCCCCAAGAGCCTCCCCCGGGAGGAG
CCTGAGTTGCGGCCCCGGCCCCCCAGGCCCCTGGACAGCCACTTGGCCATCAATGGCTCA
GCCAACCGGCAGAGCAAGTCTGAGTCGGACTACTCAGACGGTGACAATGACAGCATCAAC
AGCACGTCCAACTCCAACGATACCATCAACTGCAGCTCCGAGTCATCGTCCCGTGACAGC
CTGCGGGAGCAGACGCTCAGCAAGCAGACCTACCACAAGGAGGCCCGCAACAGCTGGGAC
TCGCCTGCCTTTAGCAACGATGTCATCCGCAAGAGGCACTACCGCATCGGCCTGAACCTC
TTCAACAAGAAGCCTGAGAAGGGAGTCCAGTACCTCATCGAGCGTGGCTTTGTGCCCGAC
ACGCCCGTCGGGGTGGCCCACTTCCTGCTGCAGCGCAAGGGCCTCAGCCGGCAGATGATC
GGCGAGTTCCTGGGCAACCGGCAGAAGCAGTTCAACCGTGACGTGCTCGACTGCGTCGTG
GACGAGATGGACTTCTCTACCATGGAGCTGGATGAGGCCCTCAGGAAATTCCAGGCGCAC
ATCCGTGTCCAAGGGGAGGCTCAGAAAGTGGAGCGGCTCATAGAGGCGTTCAGCCAGCGC
TACTGCATCTGCAACCCTGGGGTGGTGCGGCAATTCCGGAACCCAGACACCATTTTCATC
CTGGCCTTCGCCATCATCCTGCTGAACACCGACATGTACAGCCCCAATGTCAAGCCCGAG
CGGAAAATGAAGCTAGAGGACTTCATCAAGAACCTCCGAGGTGTGGACGATGGTGAGGAC
ATTCCCCGTGAGATGCTGATGGGGATCTATGAACGGATCCGTAAGCGAGAGCTAAAGACC
AATGAGGACCATGTGTCCCAGGTGCAGAAGGTGGAGAAGCTCATTGTGGGGAAAAAGCCG
ATCGGATCCCTGCATCCCGGGCTCGGCTGTGTGCTCTCTCTGCCCCACCGTCGGTTGGTC
TGCTACTGCCGGCTCTTTGAGGTTCCAGACCCAAACAAGCCCCAGAAACTCGGACTACAC
CAGCGAGAAATCTTCCTGTTCAACGACCTCCTGGTGGTCACCAAGATCTTCCAGAAGAAG
AAGAACTCGGTGACGTACAGCTTCCGACAGTCCTTCTCCTTGTACGGCATGCAGGTCCTG
CTCTTCGAGAACCAGTACTACCCCAATGGCATCCGGCTCACCTCGTCTGTCCCCGGAGCA
GATATCAAAGTGTTAATAAACTTCAACGCCCCCAACCCTCAAGACCGGAAGAAATTCACC
GATGACCTGCGGGAGTCCATTGCGGAAGTCCAAGAGATGGAGAAGCACAGGATAGAGTCG
GAGCTCGAGAAGCAGAAAGGCGTCGTGCGGCCCAGCATGTCCCAGTGCTCTAGCCTCAAA
AAGGAGTCGGGCAACGGAACACTGAGCCGGGCCTGCCTGGACGACAGCTATGCCAGCGGT
GAGGGCCTCAAGCGCAGCGCCCTCAGCAGCTCCCTGCGGGACCTCTCGGAAGCCGGGAAG
CGAGGGCGTCGCAGCAGTGCGGGATCGCTAGAGAGCAATGTGGAAGGGTCCATCATTAGC
AGTCCTCACATGCGCCGGAGAGCTACATCAACACGAGAGTGTCCATCTCGCCCACACCAG
ACTATGCCCAACTCATCTTCCCTCCTGGGCTCCTTATTCGGGAGCAAGAGAGGGAAGCCC
CCTCCCCAGGCCCACCTGCCCTCAGCCCCAGCCCTGCCACCCCCCCACCCACCGGTGGTC
CTGCCTCACTTGCAGCACTCTGTGGCTGGCCACCACCTGGGGCCCCCAGAGGGGCTGCCG
CAGGCCGCCATGCACGGGCATCACACCCAGTACTGCCACATGCAGAACCCTCCCCCGTAC
CACCATCACCACCACCACCACCCACCCCAGCACATCCAGCACGCACACCAGTACCACCAC
GGCCCCCATGGGGGCCACCCAGCCTACGGGGCCCATGCCCACGGCCACCCGCCGCTGCCC
TCGGCCCACGTGGGGCACACAGTGCACCACCATGGGCAGCCCCCTGCCCCGCCGCCCCCC
ACCAGCAGCAAGGCCAAACCCAGCGGCATCAGCACAATTGTGTTG
Protein sequence predicted based on nucleotide sequence
MACRRRYFVEGEAPSSETGTSLDSPSAYPQGPLVPGSSLSPDHYEHTSVGAYGLYSGPPG QQQRTRRPKLQHSTSILRKQAEEEAIKRSRSLSESYELSSDLQDKQVEMLERKYGGRLVT RHAARTIQTAFRQYQMNKNFERLRSSMSENRMSRRIVLSNMRMQFSFEGPEKVHSSYFEG KQVSVTNDGSQLGALVSPECGDLSEPTTLKSPAPSSDFADAITELEDAFSRQVKSLAESI DDALNCRSLHTEEAPALDAARARDTEPQTALHGMDHRKLDEMTASYSDVTLYIDEEELSP PLPLSQAGDRPSSTESDLRLRAGGAAPDYWALAHKEDKADTDTSCRSTPSLERQEQRLRV EHLPLLTIEPPSDSSVDLSDRSERGSLKRQSAYERSLGGQQGSPKHGPHSGAPKSLPREE PELRPRPPRPLDSHLAINGSANRQSKSESDYSDGDNDSINSTSNSNDTINCSSESSSRDS LREQTLSKQTYHKEARNSWDSPAFSNDVIRKRHYRIGLNLFNKKPEKGVQYLIERGFVPD TPVGVAHFLLQRKGLSRQMIGEFLGNRQKQFNRDVLDCVVDEMDFSTMELDEALRKFQAH IRVQGEAQKVERLIEAFSQRYCICNPGVVRQFRNPDTIFILAFAIILLNTDMYSPNVKPE RKMKLEDFIKNLRGVDDGEDIPREMLMGIYERIRKRELKTNEDHVSQVQKVEKLIVGKKP IGSLHPGLGCVLSLPHRRLVCYCRLFEVPDPNKPQKLGLHQREIFLFNDLLVVTKIFQKK KNSVTYSFRQSFSLYGMQVLLFENQYYPNGIRLTSSVPGADIKVLINFNAPNPQDRKKFT DDLRESIAEVQEMEKHRIESELEKQKGVVRPSMSQCSSLKKESGNGTLSRACLDDSYASG EGLKRSALSSSLRDLSEAGKRGRRSSAGSLESNVEGSIISSPHMRRRATSTRECPSRPHQ TMPNSSSLLGSLFGSKRGKPPPQAHLPSAPALPPPHPPVVLPHLQHSVAGHHLGPPEGLP QAAMHGHHTQYCHMQNPPPYHHHHHHHPPQHIQHAHQYHHGPHGGHPAYGAHAHGHPPLP SAHVGHTVHHHGQPPAPPPPTSSKAKPSGISTIVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
