Gene:
Gene Symbol:
SRCAP
Gene Name:
Snf2-related CREBBP activator protein
Original Clone ID:
U8104DG110-131
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00946252
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 9693nts
Open reading frame: 1 to 9693
ATGCAGAGCAGCCCCTCCCCTGCTCACCCTCAGCTCCCAGTCCTACAGACACAGATGGTG
TCGGACGGCATGACAGGCAGCAATCCTGTGTCCCCTGCCTCATCCAGTTCCCCAGCCTCT
AGTGGGGCAGGCGGCATCTCCCCGCAGCACATAGCTCAAGATTCCTCACTGGATGGACCT
CCAGGCCCCCCAGATGGTGCCACAGTGCCCCTGGAGGGGTTCAGCTTATCCCAGGCTGCT
GACCTGGCTAACAAGGGCCCGAAGTGGGAGAAGAGCCATGCCGAAATTGCAGAACAGGCC
AAGCATGAGGCCGAGATCGAGACTCGGATTGCTGAGCTGCGGAAGGAGGGTTTCTGGTCA
CTGAAGAGGCTGCCTAAGGTGCCAGAGCCCCCTCGCCCCAAAGGTCACTGGGACTATTTG
TGCGAAGAGATGCAGTGGCTCTCTGCTGACTTTGCTCAGGAGCGCCGTTGGAAACGGGGT
GTGGCCCGGAAGGTGGTGCGCATGGTGATCCGGCACCACGAGGAGCAGCGGCAGAAAGAG
GAACGGGCCCGGAGGGAGGAGCAGGCCAAGCTGCGTCGAATTGCTTCCACCATGGCCAAG
GATGTCAGGCAGTTCTGGAGCAATGTGGAGAAGGTGGTGCAATTCAAGCAACAGTCCCGG
CTTGAGGAAAAGCGCAAAAAAGCCCTGGACCTGCATTTGGACTTCATTGTGGGGCAAACT
GAAAAGTACTCGGACCTTCTGTCTCAGAGCCTCAACCAGCCATTAACCTCCAGCAAAGCA
GGCTCTTCCCCTTGCCTCGGCTCTTCCTCAGCTGCCTCCAGTCCTCCACCCCCTGCTTCT
CGCCTGGATGATGAAGATGGGGACTTTCAACCCCAAGAGGATGAGGAAGAGGATGATGAG
GAAACGATTGAAGTTGAAGAACAACAGGAAGGCAATGATGCAGAGGCCCAGAGGCGTGAG
ATTGAGCTGCTTCGCCGTGAGGGAGAATTGCCACTGGAAGAGCTGCTCCGTTCCCTTCCC
CCTCAGCTGTTGGAAGGGCCTTCCAGCCCCTCTCAAACCCCCTCATCTCATGATAGTGAC
ACCCGAGATGGGCCTGAAGAAGGTGCTGAAGAAGAGCCCCCTCAGGTGTTGGAGATAAAG
CCCCCACCCTCTGCTGTCACACAGCGCAACAAACAGCCTTGGCATCCAGATGAAGATGAT
GAAGAGTTTACTGCCAACGAAGAGGAAGCGGAGGATGAAGAGGATACTATAGCAGCTGAG
GAACAGTTGGAAGGGGAGGTGGATCATGCCATGGAGCTGAGCGAGTTGGCTCGAGAAGGT
GAGCTTTCCATGGAGGAGCTATTGCAGCAGTATGCAGGAGCCTATGCCCCAGGCTCTGGG
AGCAGTGAAGATGAGGATGAAGATGAGGTTGATGCTAATAGCTCTGACTGTGAACCAGAG
GGGCCCGTGGAAGCGGAAGAGCCTCCTCAGGAGGATAGTAGCAGTCAGTCAGACTCTGTG
GAGGACCGGAGTGAGGATGAGGAAGATGAACATTCAGAGGAGGAAGAAACAAGTGGAAGT
TCAGCATCAGAGGAATCTGAGTCTGAAGAGTCTGAGGATGCCCAATCACAGAGCCAAGCA
GATGAAGAGGAGGAAGATGATGATTTTGGGGTGGAGTACTTGCTTGCCAGGGATGAAGAG
CAGAGTGAGGCAGATGCAGGCAGTGGGCCTCCTACTCCAGGGCCCACTACTCTAGGTCCA
AAGAAAGAAATTACTGACATTGCTGCAGCAGCTGAAAGTCTCCAGCCCAAGGGTTACACG
CTGGCCACGACCCAGGTAAAGACGCCCATTCCCCTGCTTCTGCGGGGCCAGCTCCGGGAG
TACCAGCACATTGGGCTAGACTGGCTGGTTACCATGTATGAGAAGAAGCTTAATGGCATT
CTTGCTGATGAGATGGGGCTTGGGAAGACCATCCAGACCATCTCTCTGCTTGCCCACTTG
GCTTGTGAGAAAGGTAACTGGGGTCCCCATTTAATCATTGTTCCCACCAGCGTGATGTTG
AACTGGGAGATGGAGTTGAAACGTTGGTGCCCCAGCTTTAAAATCCTCACTTACTATGGA
GCCCAGAAAGAGAGGAAGCTCAAGCGGCAGGGCTGGACCAAGCCCAATGCCTTTCATGTG
TGTATCACATCTTACAAGCTGGTGCTGCAGGACCACCAGGCCTTCCGTCGCAAGAACTGG
CGCTATCTCATTCTGGATGAGGCGCAGAACATCAAGAACTTCAAGTCACAGCGCTGGCAG
TCACTCCTCAACTTCAACAGCCAGAGACGCCTGCTCCTGACAGGAACTCCCTTGCAGAAC
AGCCTCATGGAGCTGTGGTCCTTGATGCACTTTTTGATGCCCCATGTCTTCCAGTCTCAT
CGCGAGTTCAAGGAGTGGTTCTCTAATCCCCTAACTGGCATGATTGAGGGCAGCCAAGAG
TATAATGAAGGTCTAGTCAAACGCCTCCACAAGGTTTTGAGGCCTTTTTTACTGCGCCGA
GTTAAGGTGGATGTTGAGAAGCAGATGCCCAAAAAGTACGAGCATGTTATCCGCTGCAGG
CTCTCCAAGCGTCAACGCTGTCTCTATGATGACTTCATGGCACAGACCACAACTAAGGAG
ACACTAGCCACAGGCCATTTCATGAGCGTCATCAACATTTTGATGCAGCTGAGAAAAGTT
TGCAATCATCCAAATCTGTTCGACCCTCGACCGGTTACCTCCCCTTTCATCACCCCAGGC
ATCTGCTTCAGCACCGCCTCTCTGGTGCTAAGGGCCACGGATGTCCATCCCCTCCAGCGG
ATAGACATGGGTCGATTTGACCTTATTGGCCTGGAAGGTCGTGTCTCTCGATATGAGGCA
GACACATTTCTGCCCCGGCACCGCCTCTCTCGCCGGGTACTGTTAGAAGTGGCTACTGCT
CCTGACCCCCCACCCCGGCCCAAGCCAGTCAAGATGAAGGTCAACAGGATGCTGCAGCCA
GTACCTAAGCAAGAAGGCCGGACAGTGGTGGTGGTGAACAACCCACGGGCGCCCCTGGGC
CCTGTCCCAGTTCGACCTCCTCCAGGTCCTGAGCTCTCAGCCCAGCCCACCCCTGGCCCA
GTCCCCCAAGTGCTGCCAGCATCACTGATGGTTTCAGCCTCACCTGCCGGGCCCCCGCTT
ATTCCTGCATCTCGGCCTCCTGGCCCTGTCCTCTTGCCTCCACTGCAGCCCAACAGTGGT
TCTCTCCCCCAGGTGTTGCCATCCCCCCTGGGGGTCCTGAGTGGGACCTCACGGCCTCCC
ACGCCAACCTTGTCCCTAAAGCCAACACCACCTGCCCCAGTTCGCCTGAGCCCAGCCCCA
CCTCCAGGCTCCTCTAGCCTGTTGAAGCCCCTGACAGTGCCACCAGGCTACACCTTCCCT
CCTGCTGCTGCCACCACCACTTCTACCACCACGGCAACTGCTACCACCACAGCAGTGCCA
GCTCCGACTCCTGCACCACAGCGCCTCATTCTATCTCCCGATATGCAGGCTCGCCTGCCC
TCAGGCGAAGTGGTCAGCATCGGGCAGTTAGCCTCACTGGCACAACGTCCAGTGGCTAAT
GCAGGGGGAAGCAAACCTCTCACCTTCCAAATCCAGGGCAACAAGCTGACTTTGACTGGT
GCCCAGGTGCGCCAGCTTGCTGTGGGGCAGCCCCGCCCGCTGCAAAGGAATGTGGTGCAC
CTCGTGTCAGCAGGGGGGCAGCACCATCTCATCAGCCAGCCTGCCCATGTGGCCCTCATC
CAGGCCGTGGCCCCGACCCCTGGCCCTACCCCTGTCTCTGTGCTGCCTTCTTCGACCCCC
AGCACCACCCCTGCCCCTACTGGCCTCAGCCTTCCGCTTGCTGCTAACCAGGTGCCACCA
ACCATGGTGAATAATACAGGCGTGGTGAAGATTGTAGTGAGACAAGCCCCTCGGGATGGA
CTGACTCCTGTTCCTCCATTGGCCCCAGCACCCCGGCCTCCGAGCTCTGGGCTTCCAGCT
GTGTTGAATCCACGCCCCACGTTAACCCCTGGCCGGCTACCCACACCTACTCTGGGTACT
GCTCGAGCCCCCATGCCCACACCCACTCTGGTGAGGCCTCTTCTCAAGCTGGTCCACAGT
CCTTCACCTGAAGTCAGTGCTTCAGCCCCCGGAGCTGCCCCCTTGACCATCTCTTCTCCT
CTCCACGTGCCATCCTCACTCCCTGGGCCAGCCTCTTCTCCAATGCCAATTCCCAACTCC
TCTCCCCTTGCTAGTCCTGTGTCCTCTACAGTCTCAGTTCCATTGTCATCTTCACTCCCC
ATCTCTGTCCCCACCACACTTCCTGCCCCAGCCTCGGCTCCACTCACCATCCCCATCTCA
GCCCCCTTGACTGTTTCTGCTTCGGGCCCAGCTCTGTTGACCAGTGTGACTCCACCATTG
GCACCTGTTGTCCCAGCGGCTCCTGGACCTCCCTCCTTGGCACCATCTGGTGCTTCCCCG
TCAGCATCAGCCTTGACTCTAGGTTTGGCCACAGCTCCATCCCTGTCTTCATCTCAGACA
CCTGGTCACCCTCTGTTGTTGGCTCCCACCTCTTCACATGTTCCAGGGTTGAACTCAACC
GTGGCCCCAGCATGCTCACCTGTCCTGGTGCCAGCTTCGGCTCTGGCCAGTCCTTTTCCG
TCAGCACCAAATCCAGCTCCAGCTCAGGCTTCCCTTCTGGCTCCAGCATCTTCTGCATCT
CAGGCTCTAGCCACCCCTCTGGCTCCTATGGCGGCTCCACAGACAGCAATTCTGGCTCCT
TCTCCAGCTCCTCCTCTGGCTCCTCTTCCGGTCCTGGCACCATCGCCAGGTGCTGCTCCT
GTCCTGGCTTCATCACAGACTCCGGTTCCAGTTATGGCTCCATCGTCTACTCCAGGAACC
TCTTTAGCCTCAGCTTCACCGGTACCAGCTCCAACCCCTGTGTTGGCTCCATCATCAACT
CAAACTATGCTACCAGCCCCGGTTCCGTCACCTCTCCCGAGCCCGGCTTCTACGCAGACA
CTGGCCCTAGCCCCAGCTTTAGCACCCACTCTTGGAGGCTCATCTCCATCTCAGACACTC
TCTTTGGGAACGGGGAACCCCCAGGGACCCTTTCCAACTCAGACATTGTCATTAACTCCA
GCATCATCCCTGGTACCAACTCCAGCCCAGACACTGTCTTTGGCACCAGGACCACCACTG
GGTCCAACTCAGACGCTGTCTCTGGCTCCAGCACCCCCTCTGGCTCCAGCTTCTCCAGTG
GGCCCAGCCCCAGCTCACACGCTGACTTTGGCTCCAGCATCGTCATCTGCTTCACTCCTG
GCCCCAGCTTCAGTGCAGACACTGACCTTGAGCCCTGCCCCAGTTCCTACCCTGGGCCCG
GCCGCAGCTCAGACCTTGGCGCTGGCCCCAGCCTCCACACAGTCCCCAGCTTCCCAGGCA
TCTTCCCTTGTGGTTTCGGCATCTGGTGCCGCTCCCTTGCCTGTCACCATGGTATCCCGG
CTGCCTGTTTCCAAGGATGAGCCTGACACACTGACATTGCGCTCTGGTCCCCCCAGCCCT
CCCTCCACTGCTACCTCGTTTGGTGGCCCCCGGCCTCGACGCCAGCCCCCCCCACCACCT
CGTTCCCCTTTTTATCTGGACTCCCTGGAGGAAAAGCGGAAGCGGCAGCGGTCTGAACGC
CTGGAACGGATTTTCCAACTTAGTGAGGCTCATGGGGCCCTGGCACCTGTGTATGGGACT
GAAGTCCTGGATTTCTGTACCCTGCCCCAACCTGTTGCCAGCCCCATCGGCCCTCGTTCT
CCTGGCCCCAGCCACCCCACCTTTTGGACTTATACCGAGGCTGCCCACCGGGCTGTACTG
TTTCCCCAGCAGCGACTAGACCAGCTGTCAGAAATCATTGAGAGGTTCATCTTTGTCATG
CCTCCTGTGGAGGCACCTCCCCCTTCCCTGCATGCCTGCCACCCACCTCCTTGGCTGGCC
CCACGTCAGGCAGCCTTCCAGGAGCAATTGGCCTCTGAGCTCTGGCCCCGGGCTCGTCCT
TTGCACCGTATTGTGTGTAACATGCGCACCCAGTTCCCTGACTTAAGACTCATCCAGTAT
GATTGCGGAAAGTTGCAGACGTTGGCAGTGCTGTTGCGGCAGCTCAAGGCAGAGGGCCAC
CGAGTGCTCATCTTCACCCAGATGACCCGAATGCTGGATGTATTGGAGCAGTTTCTCACC
TACCATGGCCATCTCTACCTGCGCCTGGATGGATCTACTAGAGTTGAACAGAGACAGGCC
TTGATGGAACGGTTCAATGCAGACAAACGCATATTCTGCTTCATCCTTTCAACTCGGAGT
GGGGGTGTGGGCGTGAACCTGACAGGAGCAGACACTGTTGTTTTTTATGACAGCGACTGG
AATCCCACCATGGATGCTCAGGCCCAGGACCGCTGTCACCGAATTGGCCAGACCCGGGAT
GTCCACATATATAGGCTTATCAGTGAACGGACAGTGGAGGAGAACATCCTAAAAAAGGCA
AATCAGAAGAGAATGTTGGGGGACATGGCCATTGAGGGAGGCAACTTCACCACAGCCTAT
TTCAAACAGCAGACCATCCGAGAGCTGTTTGATATGCCCCTGGAGGAACCTTCTAGCTCA
TCCGTGCCCTCTGCCCCTGAAGAGGAGGAAGAGACTGTGGCCAGCAAGCAGACTCATATT
CTGGAGCAGGCATTGTGTCGGGCAGAAGATGAAGAGGATATCCGTGCAGCCACCCAGGCC
AAGGCTGAACAGGTGGCTGAGCTTGCAGAATTTAATGAGAACGATGGGTTTCCTGCTGGT
GAGGGAGAGGAAGCTGGCCGGCCTGGGGCTGAGGATGAGGAGATGTCCCGGGCTGAGCAG
GAAATTGCTGCCCTCGTAGAACAGCTGACCCCCATTGAGCGCTATGCCATGAAATTCCTG
GAGGCCTCACTGGAGGAGGTGAGCCGAGAGGAGCTCAAACAGGCAGAAGAGCAAGTGGAA
GCTGCCCGCAAAGACCTGGACCAAGCCAAGGAGGAGGTGTTCCGCCTACCCCAAGAGGAG
GAGGAGGGGCCGGGGGCTGGGGATGAGAGTTCCTGTGGGACTGGTGGAGGCACCCACCGG
CGCAGTAAAAAGGCCAAAGCCCCTGAGAGGCCGGGGACTCGTGTCAGTGAGCGTCTTCGT
GGAGCCCGGGCTGAGACTCAAGGGGCAAACCACACTCCTGTCATATCCGCCCATCAAACT
CGCAGCACCACCACACCACCCCGCTGCAGTCCTGCCAGGGAGCGAGTTCCCAGGCCAGCA
CCTAGGCCTCGACCCACTCCAGCTTCAGCTCCGGCTGCAATTCCTGCCCTTGTTCCTGTC
CCAGTTTCTGCCCCAGTACCCATTTCAGCCCCAAATCCAATAACCATTCTCCCTGTCCAT
ATCTTGCCTTCTCCTCCCCCTCCTTCACAGATTCCTCCTTGTTCTTCTCCTGCCTGCACC
CCTCCTCCTGCCTGTACCCCTCCACCAGCTCATACACCGCCTCCAGCCCAAACCTGTCTT
GTAACTCCTTCCTCTCCTCTCTTGCTTGGTCCACCTTCTGTGCCCATCTCTGCCTCAGTC
ACTAATCTCCCCTTGGGCTTGAGGCCTGAGGCAGAGCTGTGTGCCCAGGCATTGGCATCT
CCAGAGTCCCTGGAGCTGGCTTCTGTGGCCAGTTCAGAAACCTCCTCACTTTCTCTTGTG
CCCCCTAAAGATCTGTTGCCAGTTGCTGTGGAGATCCTGCCTGTGTCAGAGAAGAACCTT
TCTCTCACCCCTTCTGCACCCAGCCTGACCTTGGAGGCTGGCAGCATCCCCAATGGTCAA
GAGCAGGAGGCACCAGATTCTGCTGAGGGGACCACCCTTACAGTGCTGCCTGAAGGTGAG
GAGTTGCCCCTGTGTGTGAGTGAGAGCAATGGCCTGGAGCTCCCACCCTCAGCAGCATCT
GATGAGCCACTTCAGGAGCCACTGGAGGCTGACAGGACCTCGGAAGAGCTGACAGAGGCC
AAGACCCCAACCTCCAGCCCAGAGAAGCCACAGGAACTCGTTACAGCTGAGGTTGCAGCT
CCATCCACCTCATCTTCAGCCACTTCCTCGCCTGAGGGTCCTTCACCTGCCCGACCTCCT
CGGCGTCGCACCAGTGCTGATGTGGAAATTAGGGGTCAAGGGACTGGTCGGCCAGGACAA
CCACCAGGCCCCAAAGTGCTTCGAAAGCTGCCAGGACGGCTGGTAACTGTGGTAGAGGAA
AAGGAACTGGTGCGGCGGCGGCGGCAGCAGCGGGGAGCTGCCAGCACCCTAGTGCCTGGG
GTCTCTGAGACTAGTGCCAGCCCGGGAAGCCCGTCTGTCCGCAGCATGTCAGGGCCAGAA
TCCTCCCCTCCCATTGGTGGGCCCTGTGAAGCTGCTCCTTCATCCTCACTGCCCACTCCA
CCCCAGCAGCCCTTCATTGCTCGCCGTCACATTGAGCTGGGGGTGACTGGTGGTGGCAGC
CCCGAGAATGGAGACGGAGCACTGCTCGCCATCACCCCACCTGCTGTGAAACGTCGGAGG
GGGAGGCCCCCCAAGAAGAACAGGTCTCCAGCAGATGCTGGGAGAGGTGTGGATGAGGCA
CCCTCATCCACCTTGAAGGGAAAAACCAATGGGGCTGACCCAGTCCCTGGGCCTGAGACC
CTAATTGTTGCAGATCCTGTCCTGGAACCACAGCTTATTCCTGGGCCCCAGCCTCTTGGA
CCCCAGCCAGTTCACAGACCCAATCCCCTCCTGTCACCTGTGGAGAAAAGAAGGCGAGGA
CGACCCCCTAAAGCACGAGATTTGCCCATCCCTGGGACCATTTCCTCTGCAGGGGATGGC
AACTCCGAAAGTCGGACACAGCCACCCCCACACCCATCACCCCTAACCCCACTCCCACCA
CTGCTAGTTTGTCCCACTGCTACTGTTGCCAACACTGTCACCACTGTCACCATTTCAACG
TCCCCACCCAAACGGAAGAGGGGCCGACCTCCCAAGAATCCTCCATCACCTCGGCCCAGC
CAGCTCCCCGTCTTGGACCGTGACAGCACTTCTGTTCTCGAGAGCTGTGGATTGGGGAGG
CGACGGCAACCCCAGGGCCAAGGGGAGAGTGAGGGTAGTTCCTCTGATGAGGATGGAAGC
CGCCCCCTCACCCGCCTGGCCCGCCTTCGGCTTGAAGCAGAAGGAATGCGAGGACGGAAG
AGTGGAGGGTCCATGGTGGTGGCTGTAATTCAGGATGACCTGGACTTAGCAGATAGCGGG
CCAGGCGGGTTGGAATTGACACCACCTGTGGTCTCACTAACCCCAAAACTGCGCTCGACC
CGGCTGCGTCCAGGGTCTCTAGTCCCCCCACTAGAGACTGAGAAGTTGCCTCGCAAACGA
GCAGGGGCCCCAGTTGGTGGGAGTCCTGGGCTGGCAAAGCGGGGCCGCCTACAGCCCCCA
AGTCCCCTGGGGCCTGAGGGTTCAGTAGAGGAGTCTGAGGCTGAAGCCTCAGGTGAGGAG
GAGGAAGGGGATGGGACCCCACGCCGACGTCCTGGCCCCCGCCGGCTTGTTGGGACCACC
AACCAAGGGGACCAGCGCATCCTGCGCAGCAGCGCCCCTCCCTCCCTGGCTGGCCCTGCT
GTTAGTCACAGAGGCCGCAAGGCCAAGACGTTG
Protein sequence predicted based on nucleotide sequence
MQSSPSPAHPQLPVLQTQMVSDGMTGSNPVSPASSSSPASSGAGGISPQHIAQDSSLDGP PGPPDGATVPLEGFSLSQAADLANKGPKWEKSHAEIAEQAKHEAEIETRIAELRKEGFWS LKRLPKVPEPPRPKGHWDYLCEEMQWLSADFAQERRWKRGVARKVVRMVIRHHEEQRQKE ERARREEQAKLRRIASTMAKDVRQFWSNVEKVVQFKQQSRLEEKRKKALDLHLDFIVGQT EKYSDLLSQSLNQPLTSSKAGSSPCLGSSSAASSPPPPASRLDDEDGDFQPQEDEEEDDE ETIEVEEQQEGNDAEAQRREIELLRREGELPLEELLRSLPPQLLEGPSSPSQTPSSHDSD TRDGPEEGAEEEPPQVLEIKPPPSAVTQRNKQPWHPDEDDEEFTANEEEAEDEEDTIAAE EQLEGEVDHAMELSELAREGELSMEELLQQYAGAYAPGSGSSEDEDEDEVDANSSDCEPE GPVEAEEPPQEDSSSQSDSVEDRSEDEEDEHSEEEETSGSSASEESESEESEDAQSQSQA DEEEEDDDFGVEYLLARDEEQSEADAGSGPPTPGPTTLGPKKEITDIAAAAESLQPKGYT LATTQVKTPIPLLLRGQLREYQHIGLDWLVTMYEKKLNGILADEMGLGKTIQTISLLAHL ACEKGNWGPHLIIVPTSVMLNWEMELKRWCPSFKILTYYGAQKERKLKRQGWTKPNAFHV CITSYKLVLQDHQAFRRKNWRYLILDEAQNIKNFKSQRWQSLLNFNSQRRLLLTGTPLQN SLMELWSLMHFLMPHVFQSHREFKEWFSNPLTGMIEGSQEYNEGLVKRLHKVLRPFLLRR VKVDVEKQMPKKYEHVIRCRLSKRQRCLYDDFMAQTTTKETLATGHFMSVINILMQLRKV CNHPNLFDPRPVTSPFITPGICFSTASLVLRATDVHPLQRIDMGRFDLIGLEGRVSRYEA DTFLPRHRLSRRVLLEVATAPDPPPRPKPVKMKVNRMLQPVPKQEGRTVVVVNNPRAPLG PVPVRPPPGPELSAQPTPGPVPQVLPASLMVSASPAGPPLIPASRPPGPVLLPPLQPNSG SLPQVLPSPLGVLSGTSRPPTPTLSLKPTPPAPVRLSPAPPPGSSSLLKPLTVPPGYTFP PAAATTTSTTTATATTTAVPAPTPAPQRLILSPDMQARLPSGEVVSIGQLASLAQRPVAN AGGSKPLTFQIQGNKLTLTGAQVRQLAVGQPRPLQRNVVHLVSAGGQHHLISQPAHVALI QAVAPTPGPTPVSVLPSSTPSTTPAPTGLSLPLAANQVPPTMVNNTGVVKIVVRQAPRDG LTPVPPLAPAPRPPSSGLPAVLNPRPTLTPGRLPTPTLGTARAPMPTPTLVRPLLKLVHS PSPEVSASAPGAAPLTISSPLHVPSSLPGPASSPMPIPNSSPLASPVSSTVSVPLSSSLP ISVPTTLPAPASAPLTIPISAPLTVSASGPALLTSVTPPLAPVVPAAPGPPSLAPSGASP SASALTLGLATAPSLSSSQTPGHPLLLAPTSSHVPGLNSTVAPACSPVLVPASALASPFP SAPNPAPAQASLLAPASSASQALATPLAPMAAPQTAILAPSPAPPLAPLPVLAPSPGAAP VLASSQTPVPVMAPSSTPGTSLASASPVPAPTPVLAPSSTQTMLPAPVPSPLPSPASTQT LALAPALAPTLGGSSPSQTLSLGTGNPQGPFPTQTLSLTPASSLVPTPAQTLSLAPGPPL GPTQTLSLAPAPPLAPASPVGPAPAHTLTLAPASSSASLLAPASVQTLTLSPAPVPTLGP AAAQTLALAPASTQSPASQASSLVVSASGAAPLPVTMVSRLPVSKDEPDTLTLRSGPPSP PSTATSFGGPRPRRQPPPPPRSPFYLDSLEEKRKRQRSERLERIFQLSEAHGALAPVYGT EVLDFCTLPQPVASPIGPRSPGPSHPTFWTYTEAAHRAVLFPQQRLDQLSEIIERFIFVM PPVEAPPPSLHACHPPPWLAPRQAAFQEQLASELWPRARPLHRIVCNMRTQFPDLRLIQY DCGKLQTLAVLLRQLKAEGHRVLIFTQMTRMLDVLEQFLTYHGHLYLRLDGSTRVEQRQA LMERFNADKRIFCFILSTRSGGVGVNLTGADTVVFYDSDWNPTMDAQAQDRCHRIGQTRD VHIYRLISERTVEENILKKANQKRMLGDMAIEGGNFTTAYFKQQTIRELFDMPLEEPSSS SVPSAPEEEEETVASKQTHILEQALCRAEDEEDIRAATQAKAEQVAELAEFNENDGFPAG EGEEAGRPGAEDEEMSRAEQEIAALVEQLTPIERYAMKFLEASLEEVSREELKQAEEQVE AARKDLDQAKEEVFRLPQEEEEGPGAGDESSCGTGGGTHRRSKKAKAPERPGTRVSERLR GARAETQGANHTPVISAHQTRSTTTPPRCSPARERVPRPAPRPRPTPASAPAAIPALVPV PVSAPVPISAPNPITILPVHILPSPPPPSQIPPCSSPACTPPPACTPPPAHTPPPAQTCL VTPSSPLLLGPPSVPISASVTNLPLGLRPEAELCAQALASPESLELASVASSETSSLSLV PPKDLLPVAVEILPVSEKNLSLTPSAPSLTLEAGSIPNGQEQEAPDSAEGTTLTVLPEGE ELPLCVSESNGLELPPSAASDEPLQEPLEADRTSEELTEAKTPTSSPEKPQELVTAEVAA PSTSSSATSSPEGPSPARPPRRRTSADVEIRGQGTGRPGQPPGPKVLRKLPGRLVTVVEE KELVRRRRQQRGAASTLVPGVSETSASPGSPSVRSMSGPESSPPIGGPCEAAPSSSLPTP PQQPFIARRHIELGVTGGGSPENGDGALLAITPPAVKRRRGRPPKKNRSPADAGRGVDEA PSSTLKGKTNGADPVPGPETLIVADPVLEPQLIPGPQPLGPQPVHRPNPLLSPVEKRRRG RPPKARDLPIPGTISSAGDGNSESRTQPPPHPSPLTPLPPLLVCPTATVANTVTTVTIST SPPKRKRGRPPKNPPSPRPSQLPVLDRDSTSVLESCGLGRRRQPQGQGESEGSSSDEDGS RPLTRLARLRLEAEGMRGRKSGGSMVVAVIQDDLDLADSGPGGLELTPPVVSLTPKLRST RLRPGSLVPPLETEKLPRKRAGAPVGGSPGLAKRGRLQPPSPLGPEGSVEESEAEASGEE EEGDGTPRRRPGPRRLVGTTNQGDQRILRSSAPPSLAGPAVSHRGRKAKTL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
