Gene:
Gene Symbol:
ARHGEF17
Gene Name:
Rho guanine nucleotide exchange factor (GEF) 17
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00946265
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6192nts
Open reading frame: 1 to 6192
ATGGCGGACGGGGCACCCCGGCCCCAGCTTTACCGCAGCGTCTCGTTCAAGCTGCTGGAG
CGCTGGAGCGGCGGCCCCGGGCTGAGGGAGGAGGACACGGACACCCCCGGCTTGAGGCGA
CGCGCCTCGTGCCGGCCGACCACGGCTGCCCGGGGCCAGCCCTCTCGGCGCGTGTCCAAG
CTGGCGTCTGGGCCCCTGGCCGCCCCCGCGCAGCCGCGCCCGCTCCGCAGCCTCTCGCCG
TCGGTTCGCCAGCTCTCCCGGCGCTTCGACGCGCCGCGTCTGGACGACGGCTCCGCTGGG
ACCCGAGACGGAGGCGTCTTACCCGCGGCCGCGGAAGAAGCGGCCGAGGGCCCAGCGCGA
GGAGCCTGGCCCAGCGTCACCGAGATGCGCAAGCTCTTCGGCGGTCCTGGCTCCAGGAGG
CCCAGCGCCGACTCTGAATCCCCAGGAACGCCCAGCCCCGACGGTGCCGCGTGGGAGCCT
CCGGCTCGGGAGTCGCGGCAGCCACCGACGCCACCCCCTCGGACATGCTTCCCCCTGGCG
GGTCTGCGTTCGGCGCGGCCCCTGACCGGGCCGGAGACCGAAGGGAGGCTGCGCCGGCCG
CAGCAGCAACAGGAGCGGGCGCAGCGTCCAGCGGATGGTTTACATTCTTGGCATATCTTC
TCCCAACCGCAGGCCGGGGCCCGGGCCTCCTGCTCCTCCTCCTCCATCGCCGCCTCCTAT
CCTGTCAGCCGCAGTCGTGCTGCCAGCTCCAGCGAGGAGGAAGAGGAGGGCCCGCCGCAG
CTGCCTGGAGCCCAGAGTCCGGCCTACCACGGCGGCCACTCCTCGGGCAGTGACGACGAC
CGAGACGGTGAGGGCGGCCACCGCTGGGGAGGGAGGCCCGGGCTCAGGCCTGGAAGCTCC
CTATTGGATCAGGACTGCAGGCCTGACAGTGATGGGTTAAATCTAAGCAGCATGAACTCA
GCAGGGGTTTCTGGGAGCCCTGAGCCCCCAACATCTCCAAGAGCCCCTAGAGAAGAAGGA
CTCCGGGAGTGGGGTAGTGGCTCTCCGCCCTGCGTCCCAGGTCCCCAGGAGGGACTTCGG
CCTATGTCTGACTCTGTGGGAGGAGCTTTCCGTGTGGCCAAGGTGAGCTTTCCCTCGTAC
CTGGCCAGCCCCGCAGGCTCCCGCGGTAGCAGCCGTTATTCCAGCACGGAGACCCTCAAG
GACGACGACCTATGGTCTAGTAGGGGTTCTGGGGGCTGGGGCGTGTACCGCTCCCCTAGC
TTTGGAGCTGGGGAAGGGCTCCTGCGGTCCCAGGCTCGAACCCGTGCCAAAGGACCTGGA
GGCACCTCTAGGGCATTGAGGGATGGAGGATTTGAGCCTGAAAAGAGTCGACAGCGGAAG
TCCCTGTCAAATCCAGATATCGCCTCAGAGACCCTGACGCTTCTCAGTTTCCTGCGCTCA
GACCTTTCAGAGCTGAGGGTCCGAAAACCTGGTGGGAGCTCCGGGGACCGTGGAAGCAAC
CCCCTAGATGGCAGAGACTCACCATCCGCAGGTGGCCCTGTGGGGCAACTTGAACCCATA
CCCATCCCAGCCCCAGCATCACCTGGCACGCGCCCCACACTCAAGGACTTGACAGCCACT
CTGCGGAGAGCAAAGTCATTCACCTGCTCTGAGAAGCCCATGGCCCGCCGCCTGCCCCGC
ACCAGTGCTCTGAAGTCCAGCTCCTCCGAGCTCCTGCTCACAGGCCCTGGTGCCGAGGAG
GATCCGCTGCCCCTCATCGTCCAGGACCAATATGTGCAGGAGGCCCGCCAGGTTTTTGAG
AAGATCCAGCGCATGGGTGCCCAACAAGATGATGGAAGCGATGCCCCCCCTGGAAGCCCT
GACTGGGCAGGGGATGTGACCCGAGGGCAGCGGTCCCAGGAGGAGCTCTCAGGCCCTGAG
TCCAGTCTGACAGATGAAGGCATTGGGGCAGACCCTGAGCCTCCTGTTGCAGCATTTTGC
GGCCTGGGTACCACAGGGATGTGGCGACCTCTTTCCTCATCCTCGGCCCAGACGAACCAC
CATGGCCCTGGGACTGAGGACAGTCTGGGCGGGTGGGCCCTGGTGTCGCCTGAGACCCCT
CCCACACCAGGTGCCCTCCGCCGACGACGCAAAGTCCCACCTTCAGGTTCTGGTGGGAGC
GAATTGAGCAATGGGGAGGCAGGGGAGGCCTACAGGTCCCTGAGTGACCCAATTCCTCAG
CGCCACCGGGCTGCCACCTCTGAAGAGCCTACTGGGTTCTCTGTGGACAGCAACCTCCTG
GGCTCACTGAGCCCCAAGACAGGGCTCCCTGCCACCTCAGCCATGGATGAGGGCTTGACC
AGTGGTCACAGTGACTGGTCTGTGGGCAGTGAAGAGAGCAAGGGATATCAGGAGGTTATT
CAGAGCATAGTTCAGGGGCCTGGCACCCTGGGGCGTGTGGTGGACGACAGGATTGCTGGC
AAAGCCCCCAAGAAGAAATCCCTGAGTGACCCCAGCCGCCGTGGGGAGCTGGCTGGGCCT
GGATTCGAGGGCCCTGGAGGGGAGCCCATCCGAGAAGTTGAGCCCATGCTGCCTCCATCC
AGCAGCGAGCCCATCCTTGTAGAGCAGCGGGCAGAGCCAGAAGAACCTGGTGCCACCAGG
AGCCGGGCACAGTCTGAAAGGGCCCTACCTGAGGCTCTGCCTCCCCCTGCCACTGCCCAC
CGAAACTTTCACCTTGACCCCAAGCTGGCTGACATTCTGTCCCCGAGGCTAATCCGCCGA
GGCTCCAAGAAGCGCCCAGCTCGGAGTAGTCACCAGGAGCTTCGGAGAGACGAGGGCAGT
CAGGACCAGACTGGCAGCCTGTCTCGGGCCCGGCCCTCCTCCAGACACGTTCGCCATGCC
AGTGTGCCCGCCACATTTATGCCTATTGTGGTGCCTGAGCCACCAACTTCTGTTGGTCCC
CCTGTGGCTGTGCCAGAACCCATAGGCTTCCCTACCCGAGCCCATCCCACGTTGCAGGCA
CCATCGCTCGAGGACGTCACCAAGCAGTACATGCTGAACCTGCACTCCGGTGAGGTCCCT
GCCCCAGTGCCAGTGGACATGCCCTGCTTGCCTCTGGCTGCACCGCCCTCTGCTGAGGCC
AAGCCCCCTGAGGCAGCTCGGCCTGCAGATGAGCCTACCCCTGCCAGCAAGTGCTGCAGC
AAGCCACAGGTGGACATGCGGAAGCACGTGGCCATGACCCTGCTGGACACAGAGCAGTCG
TATGTGGAGTCGCTGCGCACCCTGATGCAGGGCTACATGCAGCCGCTGAAGCAGCCAGAG
AACTCCGTGCTCTGTGACCCTTCACTGGTGGACGAGATCTTCGACCAGATCCCCGAGCTC
CTGGAGCACCACGAGCAATTCCTGGAGCAGGTTCGGCACTGCATGCAGACCTGGCATGCC
CAGCAGAAGGTGGGAGCCCTGCTCGTCCAGTCGTTCTCCAAGGATGTCCTAGTAAACATC
TATTCTGCCTATATCGATAACTTCCTCAATGCAAAGGATGCTGTGCGTGTGGCCAAGGAG
GCGAGGCCTGCCTTTCTCAAGTTCCTAGAGCAAAGCATGCGTGAGAACAAGGAGAAGCAG
GCGCTGTCTGACCTCATGATCAAGCCTGTGCAGCGGATCCCACGCTACGAGCTTCTGGTG
AAGGACCTCCTGAAGCATACACCTGAGGACCACCCGGACCATCCACTCCTGCTGGAGGCG
CAGCGGAACATCAAGCAGGTGGCTGAGCGCATCAACAAGGGTGTGCGGAGTGCCGAGGAG
GCGGAGCGCCATGCCCGTGTGCTGCAGGAGATAGAGGCTCACATCGAGGGCATGGAGGAT
CTCCAGGCCCCTCTGCGGCGGTTCCTGAGACAGGAGATGGTCATTGAAGTGAAGGCGATC
GGTGGCAAGAAGGACCGGTCTCTCTTCCTGTTCACGGACCTCATCGTCTGCACCACTCTG
AAGCGAAAGTCAGGCTCCCTGCGGCGCAGCTCCATGAGCCTGTACACGGCAGCCAGTGTC
ATTGACACAGCCAGCAAGTACAAGATGCTGTGGAAGCTGCCGCTGGAAGACGCAGACATC
ATCAAAGGGGCATCCCAAGCCACCAATCGGGAGAACATCCAGAAGGCCATCAGCCGCCTT
GATGAGGACCTCACCACCCTGGGCCAAATGAGCAAGCTCTCTGAGAGCCTTGGTTTCCCC
CACCAGAGCCTGGACGATGCACTGCGGGACCTCTCAGCTGCCATGCACCGGGACCTGTCG
GAGAAGCAGGCGCTGTGCTACGCGCTTTCCTTCCCGCCAACCAAGCTGGAGCTGTGCGCC
ACTCGGCCCGAGGGCACCGACTCCTACATTTTTGAGTTCCCTCACCCTGACGCCCGCCTT
GGTTTTGAACAGGCCTTCGATGAGGCCAAGAGGAAGCTGGCATCCAGCAAAAGCTGTCTA
GACCCTGAGTTCCTGAAGGCCATCCCCATCATGAAAACCCGCAGTGGCATGCAGTTCTCC
TGTGCGGCTCCCACCCTGAACAGCTGCCCGGAGCCCTCGCCTGAGGTATGGGTCTGCAAC
AGCGACGGCTACGTGGGCCAGGTGTGCCTGCTGAGCCTGCGCGCCGAGCCGGACGTGGAG
GCCTGCATCGCCGTCTGTTCCGCCCGCATCCTCTGCATCGGGGCGGTGCCCGGGCTGCAG
CCTCGCTGCCACCGGGAGCCTCCTCCGTCGCTGAGGAGTCCTCCAGAGACGGCACCGGAG
CCCGCCGGGCCGGAGCTGGACGTCGAGGCCGCTGCAGACGAGGAAGCCGCGACGCTCGCG
GAGCCGGGGCCGCAGCCCTGCCTTCACATCTCCATTGCAGGCTCGGGCTTGGAGATGACG
CCGGGCCTCGGCGAGGGTGACCCCCGCCCAGAGCTGGTGCCCTTTGACAGTGACTCTGAC
GATGAGTCTTCGCCCAGCCCCTCGGGGACGCTGCAGAGCCAGGCCAGCCGGTCCACCATC
TCCTCCAGCTTTGGCAATGAGGAGACCCCGAGTTCCAAGGAGGCCACGGCAGAGACCACC
AGCTCAGAGGAGGAGCAGGAGCCAGGCTTCCTGCCACTGTCTGGCTCCTTTGGGCCTGGT
GGTCCCTGCGGCACCAGCCCAATGGATGGGAGAGCCCTTCGCCGCTCCAGCCACGGCTCC
TTCACCCGGGGCAGCCTTGAGGACCTGCTGAGTGTCGACCCTGAGGCCTACCAGAGCTCC
GTGTGGCTGGGCACTGAGGATGGCTGTGTCCACGTGTACCAGTCCTCCGACAGCATCCGT
GACCGCAGGAACAGCATGAAGCTCCAGCATGCGGCCTCTGTGACCTGCATCTTGTATCTG
AATAACCAGGTGTTTGTGTCTCTGGCCAATGGAGAGCTTGTGGTCTACCAAAGGGAAGCA
GGCCATTTCTGGGACCCCCAGAACTTCAAATCAGTGACCTTGGGCACCCAGGGGAGCCCC
ATCACCAAGATGGTATCTGTGGGTGGGCGGCTGTGGTGTGGCTGCCAGAACCGAGTCCTT
GTCCTGAGCCCTGACACGCTGCAGCTGGAGCACATGTTTTACGTGGGTCAGGATTCAAGC
CGCTGCGTGGCTTGCATGGTGGACTCCAGCCTGGGTGTGTGGGTGACATTGAAAGGTAGT
GCCCACGTGTGTCTCTACCATCCAGACACCTTTGAGCAGCTGGCAGAAGTAGACGTCACT
CCTCCCGTGCACAGGATGCTGGCAGGCTCGGATGCCATCATCCGGCAGCACAAGGCTGCC
TGTCTGCGAATCACAGCGCTGCTGGTGTGTGAGGAGCTGCTGTGGGTGGGCACCAGTGCT
GGTGTCGTCCTCACCATGCCCACTTCGCCCGGTACTGTCAGCTGCCCACGGGCACCACTC
AGTCCCACAGGCCTCGGCCAGGGACACACCGGCCACGTCCGCTTCTTGGCTGCAGTCCAG
CTGCCAGATGGCTTCAACCTGCTCTGCCCAACCCCACCACCTCCCCCAGACACAGGCCCC
GAGAAGCTGCCATCACTGGAGCACCGGGACTCCCCTTGGCACCGAGGCCCCGCCCCTGCC
AGGCCTAAAATGCTGGTTATCAGTGGAGGTGATGGCTATGAGGACTTCCGACTCAGCAGT
GGGGGCGGCAGCAGCAGTGAGACTGTGGGTCGAGACGACAGCACAAACCACCTCCTCCTG
TGGAGGGTGTTG
Protein sequence predicted based on nucleotide sequence
MADGAPRPQLYRSVSFKLLERWSGGPGLREEDTDTPGLRRRASCRPTTAARGQPSRRVSK LASGPLAAPAQPRPLRSLSPSVRQLSRRFDAPRLDDGSAGTRDGGVLPAAAEEAAEGPAR GAWPSVTEMRKLFGGPGSRRPSADSESPGTPSPDGAAWEPPARESRQPPTPPPRTCFPLA GLRSARPLTGPETEGRLRRPQQQQERAQRPADGLHSWHIFSQPQAGARASCSSSSIAASY PVSRSRAASSSEEEEEGPPQLPGAQSPAYHGGHSSGSDDDRDGEGGHRWGGRPGLRPGSS LLDQDCRPDSDGLNLSSMNSAGVSGSPEPPTSPRAPREEGLREWGSGSPPCVPGPQEGLR PMSDSVGGAFRVAKVSFPSYLASPAGSRGSSRYSSTETLKDDDLWSSRGSGGWGVYRSPS FGAGEGLLRSQARTRAKGPGGTSRALRDGGFEPEKSRQRKSLSNPDIASETLTLLSFLRS DLSELRVRKPGGSSGDRGSNPLDGRDSPSAGGPVGQLEPIPIPAPASPGTRPTLKDLTAT LRRAKSFTCSEKPMARRLPRTSALKSSSSELLLTGPGAEEDPLPLIVQDQYVQEARQVFE KIQRMGAQQDDGSDAPPGSPDWAGDVTRGQRSQEELSGPESSLTDEGIGADPEPPVAAFC GLGTTGMWRPLSSSSAQTNHHGPGTEDSLGGWALVSPETPPTPGALRRRRKVPPSGSGGS ELSNGEAGEAYRSLSDPIPQRHRAATSEEPTGFSVDSNLLGSLSPKTGLPATSAMDEGLT SGHSDWSVGSEESKGYQEVIQSIVQGPGTLGRVVDDRIAGKAPKKKSLSDPSRRGELAGP GFEGPGGEPIREVEPMLPPSSSEPILVEQRAEPEEPGATRSRAQSERALPEALPPPATAH RNFHLDPKLADILSPRLIRRGSKKRPARSSHQELRRDEGSQDQTGSLSRARPSSRHVRHA SVPATFMPIVVPEPPTSVGPPVAVPEPIGFPTRAHPTLQAPSLEDVTKQYMLNLHSGEVP APVPVDMPCLPLAAPPSAEAKPPEAARPADEPTPASKCCSKPQVDMRKHVAMTLLDTEQS YVESLRTLMQGYMQPLKQPENSVLCDPSLVDEIFDQIPELLEHHEQFLEQVRHCMQTWHA QQKVGALLVQSFSKDVLVNIYSAYIDNFLNAKDAVRVAKEARPAFLKFLEQSMRENKEKQ ALSDLMIKPVQRIPRYELLVKDLLKHTPEDHPDHPLLLEAQRNIKQVAERINKGVRSAEE AERHARVLQEIEAHIEGMEDLQAPLRRFLRQEMVIEVKAIGGKKDRSLFLFTDLIVCTTL KRKSGSLRRSSMSLYTAASVIDTASKYKMLWKLPLEDADIIKGASQATNRENIQKAISRL DEDLTTLGQMSKLSESLGFPHQSLDDALRDLSAAMHRDLSEKQALCYALSFPPTKLELCA TRPEGTDSYIFEFPHPDARLGFEQAFDEAKRKLASSKSCLDPEFLKAIPIMKTRSGMQFS CAAPTLNSCPEPSPEVWVCNSDGYVGQVCLLSLRAEPDVEACIAVCSARILCIGAVPGLQ PRCHREPPPSLRSPPETAPEPAGPELDVEAAADEEAATLAEPGPQPCLHISIAGSGLEMT PGLGEGDPRPELVPFDSDSDDESSPSPSGTLQSQASRSTISSSFGNEETPSSKEATAETT SSEEEQEPGFLPLSGSFGPGGPCGTSPMDGRALRRSSHGSFTRGSLEDLLSVDPEAYQSS VWLGTEDGCVHVYQSSDSIRDRRNSMKLQHAASVTCILYLNNQVFVSLANGELVVYQREA GHFWDPQNFKSVTLGTQGSPITKMVSVGGRLWCGCQNRVLVLSPDTLQLEHMFYVGQDSS RCVACMVDSSLGVWVTLKGSAHVCLYHPDTFEQLAEVDVTPPVHRMLAGSDAIIRQHKAA CLRITALLVCEELLWVGTSAGVVLTMPTSPGTVSCPRAPLSPTGLGQGHTGHVRFLAAVQ LPDGFNLLCPTPPPPPDTGPEKLPSLEHRDSPWHRGPAPARPKMLVISGGDGYEDFRLSS GGGSSSETVGRDDSTNHLLLWRVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
