Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00961016
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 3981nts
Open reading frame: 1 to 3981
ATGACCGAGGAGAGCTCTGACGTTCCCAGGGAGTTGATAGAAAGCATAAAGGATGTTATT
GGCAGAAAGATAAAAATTTCAGTGAAGAAGAAAGTAAAGTTGGAAGTTAAGGGAGACAAA
GTTGAAAACAAAGTGCTGGTGCTTACATCATGCCGAGCCTTCCTTGTAACAGCGCGAATC
CCCACCAAGCTCGAGTTAACCTTCAGCTACTTGGAGATTCATGGCGTCGTTTGCAGCAAG
TCAGCTCAGATGATTGTGGAAACTGAGAAGTGCAGCATTTCCATGAAGATGGCGTCGCCC
GAGGACGTGAGTGAGGTGCTGGCTCACATAGGCACCTGCCTGAGGAAGATATTTCCTGGC
CTCTCTCCAGTGAGAATCATGAAAAAAGTCTCCATGGAGCCATCTGAGCGCCTGGCTAGT
CTCCAGGCGCTGTGGGACAGCCAGACCGTGGCTGAGCAGGGCCCCTGTGGTGGATTTTCT
CAGATGTATGCCTGTGTTTGTGACTGGCTTGGATTTTCATACAGGGAAGAAGTACAATGG
GATGTGGATACAATTTATCTTACCCAAGACACCAGGGAATTGAATTTACAAGATTTTAGT
CATCTTGACCACAGGGACCTAATACCTATCATTGCTGCTCTGGAATATAATCAGTGGTTC
ACAAAACTGTCCTCTAAGGATCTAAAACTGTCCACTGATGTCTGTGAACAGATCTTGAGG
GTGGTGAGTAGGTCCAATCGACTGGAAGAATTGGTGTTGGAAAATGCTGGACTTAGAACA
GATTTTGCACAAAAACTGGCCAGTGCTCTAGCACATAATCCCAACTCAGGACTCCACACA
ATTAACCTTGCTGGCAACCCACTGGAGGATAGAGGTGTGTCCTCTTTAAGTATTCAATTT
GCCAAACTCCCAAAGGGATTAAAGCACTTAAATTTATCTAAAACCTCATTATCACCTAAA
GGGGTGAACAGCCTTTCTCAGTCACTCAGTGCCAATCCATTGACCGCCTCTACCCTTGTC
CACCTCGACCTCTCAGGGAACGTCCTTCGTGGAGATGACCTCTCACACATGTATAATTTT
TTGGCCCAGCCAAATGCCATTGTTCATCTGGATTTATCCAATACAGAATGTTCCCTGGAC
ATGGTCTGTGGAGCTCTTCTCCGTGGATGCCTTCAATATTTAGCTGTGCTCAACCTCTCC
AGAACTGTCTTCTCTCACCGGAAAGGAAAAGAAGTACCTCCATCTTTCAAGCAATTTTTT
AGTAGTTCTCTGGCTTTGATGCACATCAACCTTTCAGGCACAAAACTGTCTCCTGAGCCC
TTAAAAGCACTGTTATTGGGCCTGGCTTGTAATCATAACTTGAAAGGGGTTTCTCTGGAT
CTCAGCAACTGTGAGCTGAGATCAGGAGGTGCTCAAGTATTAGAAGGTTGCATTGCTGAA
ATACACAACATCACCAGCTTAGACATCTCTGACAATGGTTTAGAATCTGACCTATCTACC
TTAATAGTGTGGCTCAGTAAAAACAGATCAATACAACACCTGGCGTTAGGCAAAAATTTT
AATAATATGAAATCCAAAAATCTGACACCTGTATTGGACAACTTAGTACAGATGATTCAA
GATGAAGAATCACCTCTGCAGTCCTTGTCCCTGGCTGACTCGAAACTCAAGACTGAGGTC
ACCATCATCATCAATGCCCTGGGAAGCAACACCTCCCTGACCAAAGTGGACATTAGCGGC
AACGGAATGGGGGACATGGGAGCGAAGATGCTGGCCAAAGCACTGCAGATCAACACTAAG
CTCAGGACTGTAATATGGGACAAGAACAACATCACTGCACAAGGCTTTCAGGATATAGCT
GTTGCTATGGAAAAGAACTACACATTAAGATTTATGCCAATTCCTATGTATGATGCTTCT
CAAGCCCTAAAAACAAACCCTGAAAAAACAGAAGACGCTCTGCAAAAGATAGAAAACTAC
CTGCTACGAAATCACGAGACTAGAAAATACCTTCAAGAGCAGGCCTACCGCCTGCAGCAG
GGTATTGTCACCAGCACCACCCAGCAGATGATTGACAGAATATGTGTGAAAGTACAAGAT
CATCTCAACTCCTTACGAAATTGTGGGGGAGACGCTATCCAGGAAGATTTAAAATCAGCA
GAGCGGCTCATGCGTGATGCTAAGAACTCTAAAACGTTGTTACCAAATTTATACCATGTT
GGTGGTGCATCTTGGGCGGGAGCCAGTGGCTTACTATCCAGTCCAATTCAGGAGACCCTG
GAATCAATGGCTGGAGAAGTTACAAGAGTAGTAGATGAACAACTAAAGGCGTTGCTTGAG
TCCATGGTTGATGCTGCTGAGAATCTTTGTCCCAATGTGATGAAAAAAGCCCACATTCGA
CAAGACTTGATTCATGCCAGCACCGAAAAGATTTCTATTCCACGTACCTTTGTTAAAAAT
GTCCTGTTGGAGCAGTCTGGAATTGATATCCTTAACAAAATTAGTGAAGTAAAATTGACA
GTGGCCTCGTTCCTGTCTGATAGAATTGTGGATGAAATCCTGGATGCACTCTCACATTGC
CATCATAAACTGGCTGACCATTTCAGCAGACGTGGCAAGACCCTTCCTCAACAAGAATCC
TTAGAGATCGAGCTGGCTGAGGAGAAGCCAGTTAAACGTTCCATCATCACAGTGGAGGAG
CTAACAGAGATAGAGCGTTTGGAAGATCTGGATACCTGTATGATGACCCCTAAATCCAAA
AGGAAGAGTATCCATAGCCGAATGCTGCGGCCTGTTTCTAGGGCTTTTGAAATGGAGTTT
GATCTAGATAAAGCGCTGGAAGAGGTACCAATTCACATCGAAGACCCGCCCTTCCCATCC
CTCAGACAGGAGAAGCGGAGCTCGGGATTTATCTCTGAGTTGCCCTCTGAAGAGGGGAAG
AAGCTGGAACACTTTACCAAGTTAAGGCCAAAAAGGAATAAGAAGCAGCAACCCACCCAA
GCAGCGGTCTGTGCTGCCAACATAGTCTCACAAGATGGTGAACAGAATGGTCTCATGGGG
AGAGTGGATGAAGGTGTAGATGAATTTTTTACCAAGAAGGTGACCAAAATGGATTCCAAG
AAATGGTCAACAAGAGGCTCAGAGTCCCATGAGCTTAATGAAGGAGGAGATGAAAAGAAA
AAGCGAGATTCTCGGAAAAGTAGTGGCTTTCTCAATTTAATCAAATCCCGGTCCAAATCC
GAGCGACCACCAACGATCTTGATGACAGAAGAACCCTCCTCACCAAAAGGGGCAGTCAGA
AGTCCACCTGTGGACTGTCCCAGGAAGGACACAAAGGCCGCCGAGCACAATGGCAATTCT
GAACGGATAGAGGAGATAAAAACACCTGACTCCTTTGAAGAGAGTCAAGGGGAAGAAATA
GGGAAGGTGGAACGGAGTGACAGCAAGAGCAGCCCACAGGCAGGGCGGAGGTATGGGGTC
CAGGTGATGGGCAGTGGTCTGCTGGCAGAGATGAAAGCCAAGCAAGAGAACCGCTTTGGT
TTGGGAACACCAGAAAAGAATACCAAAGCAGAACCCAAAGCGGAAGCAGGCTCCAGGTCT
CGGAGCTCATCCAGCACACCTACGAGCCCGAAGCCCCTCCTGCAGTCCCCCAAACCCAGT
CTGGCAGCACGGCCCGTCATCCCGCAGAAACCAAGAACCGCCTCACGGCCTGATGACATT
CCAGACTCTCCATCTAGCCCGAAAGTTGCCCTTCTTCCACCTGTCCTGAAAAAAGTTCCT
TCAGACAAAGAGAGAGATGGCCAGAGTAGCCCCCAGCCCAGCCCCAGGACATTTTCACAG
GAAGTTTCAAGGAGAAGCTGGGGCCAGCAGGCCCAGGAGTATCAAGAACAAAAGCAACGG
TCCTCCAGTAAAGATGGCCATCAAGGCAGCAAATCTAATGACTCCGGGGAAGAAGCAGAA
AAAGAGTTTATTTTTGTGTAA
Protein sequence predicted based on nucleotide sequence
MTEESSDVPRELIESIKDVIGRKIKISVKKKVKLEVKGDKVENKVLVLTSCRAFLVTARI PTKLELTFSYLEIHGVVCSKSAQMIVETEKCSISMKMASPEDVSEVLAHIGTCLRKIFPG LSPVRIMKKVSMEPSERLASLQALWDSQTVAEQGPCGGFSQMYACVCDWLGFSYREEVQW DVDTIYLTQDTRELNLQDFSHLDHRDLIPIIAALEYNQWFTKLSSKDLKLSTDVCEQILR VVSRSNRLEELVLENAGLRTDFAQKLASALAHNPNSGLHTINLAGNPLEDRGVSSLSIQF AKLPKGLKHLNLSKTSLSPKGVNSLSQSLSANPLTASTLVHLDLSGNVLRGDDLSHMYNF LAQPNAIVHLDLSNTECSLDMVCGALLRGCLQYLAVLNLSRTVFSHRKGKEVPPSFKQFF SSSLALMHINLSGTKLSPEPLKALLLGLACNHNLKGVSLDLSNCELRSGGAQVLEGCIAE IHNITSLDISDNGLESDLSTLIVWLSKNRSIQHLALGKNFNNMKSKNLTPVLDNLVQMIQ DEESPLQSLSLADSKLKTEVTIIINALGSNTSLTKVDISGNGMGDMGAKMLAKALQINTK LRTVIWDKNNITAQGFQDIAVAMEKNYTLRFMPIPMYDASQALKTNPEKTEDALQKIENY LLRNHETRKYLQEQAYRLQQGIVTSTTQQMIDRICVKVQDHLNSLRNCGGDAIQEDLKSA ERLMRDAKNSKTLLPNLYHVGGASWAGASGLLSSPIQETLESMAGEVTRVVDEQLKALLE SMVDAAENLCPNVMKKAHIRQDLIHASTEKISIPRTFVKNVLLEQSGIDILNKISEVKLT VASFLSDRIVDEILDALSHCHHKLADHFSRRGKTLPQQESLEIELAEEKPVKRSIITVEE LTEIERLEDLDTCMMTPKSKRKSIHSRMLRPVSRAFEMEFDLDKALEEVPIHIEDPPFPS LRQEKRSSGFISELPSEEGKKLEHFTKLRPKRNKKQQPTQAAVCAANIVSQDGEQNGLMG RVDEGVDEFFTKKVTKMDSKKWSTRGSESHELNEGGDEKKKRDSRKSSGFLNLIKSRSKS ERPPTILMTEEPSSPKGAVRSPPVDCPRKDTKAAEHNGNSERIEEIKTPDSFEESQGEEI GKVERSDSKSSPQAGRRYGVQVMGSGLLAEMKAKQENRFGLGTPEKNTKAEPKAEAGSRS RSSSSTPTSPKPLLQSPKPSLAARPVIPQKPRTASRPDDIPDSPSSPKVALLPPVLKKVP SDKERDGQSSPQPSPRTFSQEVSRRSWGQQAQEYQEQKQRSSSKDGHQGSKSNDSGEEAE KEFIFV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
