Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00963968
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3579nts
Open reading frame: 1 to 3579
ATGCCCGCCCTGTGGCTGGGTTGCTGTCTGTGCTTCAGCCTGCTCCTGCCAGCAGCTCGT
GCCACATCCCGGAGAGAAGTGTGCGACTGTAACGGAAAGTCTAGACAGTGCATTTTCGAT
CGTGAGCTGCACCGGCAGACCGGTAATGGCTTTAGGTGCCTGAATTGTAACGACAATACT
GATGGCATCCACTGCGAAAAGTGTAAAAACGGGTTCTACCGACATCGTGAGCGGGACAGA
TGCCTGCCCTGCAACTGTAATAGCAAAGGAAGTCTGTCAGCCCGTTGTGATAATTCAGGC
CGGTGCAGCTGTAAACCAGGTGTGACCGGAGCTCGTTGCGACAGATGTCTGCCTGGCTTT
CATATGCTCACTGATGCAGGTTGCACCCAGGACCAGCGGCTGCTGGATAGCAAGTGCGAC
TGTGATCCTGCTGGTATCGCAGGTCCATGTGACGCAGGCCGGTGCGTGTGCAAACCAGCC
GTCACTGGAGAAAGATGCGACAGGTGTCGCTCCGGCTACTATAACCTGGATGGCGGGAAT
CCCGAGGGATGCACCCAGTGCTTCTGTTACGGTCACAGCGCCTCCTGTAGAAGCTCCGCT
GAGTATAGTGTGCACAAGATCACCTCAACATTTCATCAGGACGTGGATGGGTGGAAGGCT
GTGCAGAGGAACGGATCTCCTGCAAAACTGCAGTGGAGTCAGCGACATCAGGACGTGTTC
TCTAGTGCCCAGCGGCTGGATCCCGTGTACTTCGTCGCACCCGCCAAATTTCTGGGAAAT
CAGCAGGTGTCTTACGGCCAGTCTCTGAGTTTTGACTATAGGGTCGATCGTGGAGGCCGG
CACCCTAGCGCCCATGACGTGATTCTGGAGGGGGCAGGACTCAGGATCACAGCCCCACTG
ATGCCGCTCGGAAAGACTCTGCCATGCGGTCTCACTAAAACCTACACATTCCGCCTGAAC
GAACACCCTTCCAACAATTGGTCTCCACAGCTGAGTTACTTTGAGTATCGGCGGCTGCTG
CGTAACCTGACCGCCCTGCGAATCCGTGCTACCTACGGGGAATACAGCACAGGATACATC
GATAATGTCACTCTGATCTCCGCCAGACCCGTGAGCGGTGCTCCAGCACCTTGGGTTGAG
CAGTGCATTTGTCCAGTGGGGTACAAGGGACAGTTCTGCCAGGACTGTGCCAGCGGGTAT
AAAAGAGATTCCGCTAGGCTGGGTCCCTTCGGCACTTGCATCCCTTGCAACTGTCAGGGT
GGTGGAGCATGCGACCCTGATACCGGCGACTGTTACTCTGGCGACGAAAACCCAGATATT
GAGTGCGCTGATTGTCCCATCGGATTCTATAATGACCCCCACGATCCTAGGAGCTGCAAG
CCATGCCCGTGTCATAATGGCTTTTCATGTAGCGTCATGCCAGAGACCGAGGAAGTGGTC
TGCAACAATTGTCCACCTGGTGTGACAGGAGCTCGTTGCGAACTGTGCGCAGACGGTTAC
TTCGGCGATCCCTTTGGGGAGCACGGACCAGTGAGACCGTGCCAGCCCTGCCAGTGTAAC
AATAACGTCGACCCTTCCGCCTCTGGCAACTGTGATCGCCTGACTGGGCGATGCCTCAAG
TGTATTCATAATACCGCTGGCATCTACTGCGACCAGTGTAAAGCAGGTTATTTCGGCGAC
CCACTGGCACCAAACCCTGCTGATAAGTGCAGGGCCTGCAACTGTAATCCTATGGGGTCA
GAACCAGTTGGATGTCGCAGCGACGGTACCTGCGTGTGCAAACCAGGTTTCGGTGGTCCA
AACTGCGAGCACGGAGCCTTCAGCTGCCCAGCCTGTTATAATCAGGTGAAGATTCAGATG
GATCAGTTTATGCAGCAGCTGCAGAGAATGGAGGCCCTGATCTCCAAAGCACAGGGGGGA
GACGGCGTTGTGCCCGATACAGAGCTGGAAGGGAGGATGCAGCAGGCCGAACAGGCTCTG
CAGGACATCCTGAGAGATGCCCAGATCTCTGAGGGCGCTAGTAGGTCACTCGGGCTGCAG
CTCGCCAAGGTGCGAAGTCAGGAAAACTCTTACCAGAGTCGTCTGGACGACCTGAAAATG
ACCGTTGAGCGGGTGAGAGCCCTGGGTAGCCAGTATCAGAATAGGGTGCGCGACACACAC
AGACTGATTACTCAGATGCAGCTGTCCCTCGCAGAGTCAGAAGCCAGCCTGGGCAACACC
AATATCCCTGCCTCTGATCACTACGTCGGGCCAAACGGATTCAAGAGTCTGGCACAGGAA
GCCACAAGGCTCGCAGAATCACATGTGGAGAGCGCCTCCAATATGGAGCAGCTGACTCGA
GAGACCGAAGACTATAGTAAGCAGGCTCTGTCACTGGTGCGGAAAGCACTGCATGAGGGT
GTCGGCTCTGGGAGTGGATCACCAGATGGTGCAGTCGTTCAGGGTCTGGTGGAAAAGCTC
GAGAAGACAAAATCCCTGGCACAGCAGCTCACCCGTGAGGCTACACAGGCAGAGATTGAA
GCTGACAGATCATACCAGCACAGCCTGAGGCTCCTGGACAGCGTCTCCCGTCTGCAGGGC
GTGAGCGATCAGTCTTTCCAGGTCGAGGAAGCCAAGCGGATCAAGCAGAAAGCTGACAGC
CTGTCAAGCCTCGTGACCCGACATATGGATGAGTTTAAGCGTACACAGAAAAACCTGGGC
AATTGGAAAGAGGAAGCCCAGCAGCTCCTGCAGAACGGCAAGAGCGGCCGCGAAAAATCC
GATCAGCTCCTGTCTCGGGCCAACCTGGCTAAGTCCAGAGCACAGGAGGCCCTGTCTATG
GGCAATGCCACCTTCTACGAGGTTGAAAGCATTCTGAAAAACCTGAGGGAATTTGACCTG
CAGGTGGATAATCGCAAGGCCGAGGCTGAGGAAGCAATGAAAAGACTGAGTTATATCTCA
CAGAAGGTGAGCGACGCCTCCGATAAAACTCAGCAGGCTGAGAGGGCACTGGGATCTGCA
GCAGCTGACGCACAGCGTGCTAAGAACGGTGCAGGCGAAGCCCTGGAGATCTCCTCTGAG
ATTGAACAGGAGATCGGCAGCCTGAACCTGGAGGCTAATGTGACCGCAGATGGAGCACTG
GCCATGGAAAAGGGTCTGGCCAGCCTGAAGAGTGAGATGAGAGAAGTGGAGGGCGAACTG
GAGCGCAAGGAACTCGAGTTTGACACAAATATGGATGCAGTCCAGATGGTCATCACTGAG
GCCCAGAAGGTCGACACCAGAGCTAAAAACGCAGGCGTTACTATCCAGGACACACTGAAT
ACTCTCGATGGGCTCCTGCACCTGATGGACCAGCCCCTCAGCGTGGATGAGGAAGGCCTG
GTGCTGCTGGAACAGAAGCTCTCCCGCGCTAAAACCCAGATTAACAGTCAGCTGCGACCT
ATGATGTCAGAGCTGGAGGAACGAGCACGTCAGCAGCGTGGTCACCTGCACCTGCTGGAA
ACCTCCATCGACGGAATTCTGGCTGATGTGAAGAACCTGGAGAATATCCGGGACAACCTG
CCACCGGGCTGCTACAATACACAGGCCCTGGAGCAGCAG
Protein sequence predicted based on nucleotide sequence
MPALWLGCCLCFSLLLPAARATSRREVCDCNGKSRQCIFDRELHRQTGNGFRCLNCNDNT DGIHCEKCKNGFYRHRERDRCLPCNCNSKGSLSARCDNSGRCSCKPGVTGARCDRCLPGF HMLTDAGCTQDQRLLDSKCDCDPAGIAGPCDAGRCVCKPAVTGERCDRCRSGYYNLDGGN PEGCTQCFCYGHSASCRSSAEYSVHKITSTFHQDVDGWKAVQRNGSPAKLQWSQRHQDVF SSAQRLDPVYFVAPAKFLGNQQVSYGQSLSFDYRVDRGGRHPSAHDVILEGAGLRITAPL MPLGKTLPCGLTKTYTFRLNEHPSNNWSPQLSYFEYRRLLRNLTALRIRATYGEYSTGYI DNVTLISARPVSGAPAPWVEQCICPVGYKGQFCQDCASGYKRDSARLGPFGTCIPCNCQG GGACDPDTGDCYSGDENPDIECADCPIGFYNDPHDPRSCKPCPCHNGFSCSVMPETEEVV CNNCPPGVTGARCELCADGYFGDPFGEHGPVRPCQPCQCNNNVDPSASGNCDRLTGRCLK CIHNTAGIYCDQCKAGYFGDPLAPNPADKCRACNCNPMGSEPVGCRSDGTCVCKPGFGGP NCEHGAFSCPACYNQVKIQMDQFMQQLQRMEALISKAQGGDGVVPDTELEGRMQQAEQAL QDILRDAQISEGASRSLGLQLAKVRSQENSYQSRLDDLKMTVERVRALGSQYQNRVRDTH RLITQMQLSLAESEASLGNTNIPASDHYVGPNGFKSLAQEATRLAESHVESASNMEQLTR ETEDYSKQALSLVRKALHEGVGSGSGSPDGAVVQGLVEKLEKTKSLAQQLTREATQAEIE ADRSYQHSLRLLDSVSRLQGVSDQSFQVEEAKRIKQKADSLSSLVTRHMDEFKRTQKNLG NWKEEAQQLLQNGKSGREKSDQLLSRANLAKSRAQEALSMGNATFYEVESILKNLREFDL QVDNRKAEAEEAMKRLSYISQKVSDASDKTQQAERALGSAAADAQRAKNGAGEALEISSE IEQEIGSLNLEANVTADGALAMEKGLASLKSEMREVEGELERKELEFDTNMDAVQMVITE AQKVDTRAKNAGVTIQDTLNTLDGLLHLMDQPLSVDEEGLVLLEQKLSRAKTQINSQLRP MMSELEERARQQRGHLHLLETSIDGILADVKNLENIRDNLPPGCYNTQALEQQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
