Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
HsCD00514074
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pCellFree_c-mcherry_5800
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00964717
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1797nts
Open reading frame: 1 to 1797
ATGTCCACAGGGTTCTCCTTCGGGTCCGGGACTCTGGGCTCCACCACCGTGGCCGCCGGC
GGGACCAGCACAGGCGGCGTTTTCTCCTTCGGAACGGGAGCGTCTAGCAACCCTTCTGTG
GGGCTCAATTTTGGAAATCTTGGAAGTACTTCAACTCCAGCAACTACATCTGCTCCTTCA
AGTGGTTTTGGAACCGGGCTCTTTGGATCTAAACCTGCCACTGGGTTCACTCTAGGAGGA
ACAAATACAGGAATAGCAACAACTATAACTACAGGATTAACTCTGGGAACGCCAGCCACT
ACATCTGCAGCTACAACAGGCTTCAGTTTAGGATTCAATAAACCTGCAGCATCTGCCACA
CCATTTGCTCTACCTATTACCTCTACCTCAGCTAGCGGTCTGACTCTTTCGTCTGCTCTG
ACATCAACTCCAGCAGCATCCACAGGATTTACTCTAAATAATTTGGGTGGGACAACAGCC
ACAACTACAACTGCATCAACAGGCCTCTCTTTAGGGGGAGCCTTAGCTGGTTTGGGAGGT
TCACTTTTCCAGAGTACAAACACAGGAACATCAGGACTTGGACAGAATGCTTTAGGGTTG
ACTTTGGGAACTACAGCAGCTACTTCAACTGCAGGCAATGAAGGCCTTGGTGGTATAGAT
TTCAGTAGCTCCTCAGATAAAAAGAGTGATAAAACGGGAACAAGACCAGAGGATAGTAAA
GCTCTGAAGGATGAAAATCTACCTCCTGTCATCTGCCAGGATGTTGAAAATCTCCAGAAA
TTTGTGAAGGAGCAGAAACAAGTTCAAGAAGAAATTAGTAGAATGTCTTCAAAAGCAATG
CTTAAGGTACAAGAAGATATTAAAGCTCTGAAGCAGCTCCTGTCGTTGGCTGCCAATGGA
ATACAGAGAAACACTCTCAACATTGACAAATTGAAAATAGAAACTGCTCAGGAGTTGAAG
AATGCTGAAATAGCTTTAAGAACCCAGAAGACACCACCTGGACTTCAACATGAATATGCA
GCTCCTGCTGACTACTTCAGAATCTTGGTTCAGCAATTTGAGGTACAGCTTCAGCAGTAC
AGGCAGCAGATTGAAGAACTAGAAAACCATCTTGCCACTCAAGCAAATAATTCACATATA
ACCCCTCAAGATTTGTCAATGGCTATGCAGAAAATTTATCAAACATTTGTAGCTTTAGCG
GCACAACTTCAGTCTATTCATGAAAATGTAAAGGTTCTGAAAGAACAGTACCTTGGCTAC
AGGAAAATGTTCTTGGGAGATGCTGTTGATGTGTTTGAAACAAGGCGAGCAGAAGCCAAG
AAGTGGCAGAACACACCCAGAGTTACTACTGGACCCACTCCTTTCAGCACCATGCCAAAC
GCAGCAGCCGTTGCCATGGCTGCAACACTTACACAGCAGCAACAGCCTGCTACAGGGCCA
CAGCCATCTCTGGGAGTTAGTTTTGGAACGCCATTCGGCTCAGGTATTGGCACTGGCTTG
CAATCAAGTGGCTTAGGTTCTTCAAACCTTGGAGGATTTGGAACTAGCTCTGGTTTTGGA
TGCAGCACCACAGGGGCCTCCACATTTGGATTTGGAACAACAAATAAACCCTCAGGAAGT
CTTAGTGCAGGCTTTGGCAGCTCAAGTACATCTGGGTTTAACTTCAGCAATCCTGGCATC
ACGGCATCAGCTGGTTTGACTTTTGGGGTGTCCAATCCTGCCTCTGCAGGTTTTGGAACA
GGAGGACAACTCCTTCAGTTGAAGAAACCTCCAGCTGGAAACAAAAGAGGAAAAAGA
Protein sequence predicted based on nucleotide sequence
MSTGFSFGSGTLGSTTVAAGGTSTGGVFSFGTGASSNPSVGLNFGNLGSTSTPATTSAPS SGFGTGLFGSKPATGFTLGGTNTGIATTITTGLTLGTPATTSAATTGFSLGFNKPAASAT PFALPITSTSASGLTLSSALTSTPAASTGFTLNNLGGTTATTTTASTGLSLGGALAGLGG SLFQSTNTGTSGLGQNALGLTLGTTAATSTAGNEGLGGIDFSSSSDKKSDKTGTRPEDSK ALKDENLPPVICQDVENLQKFVKEQKQVQEEISRMSSKAMLKVQEDIKALKQLLSLAANG IQRNTLNIDKLKIETAQELKNAEIALRTQKTPPGLQHEYAAPADYFRILVQQFEVQLQQY RQQIEELENHLATQANNSHITPQDLSMAMQKIYQTFVALAAQLQSIHENVKVLKEQYLGY RKMFLGDAVDVFETRRAEAKKWQNTPRVTTGPTPFSTMPNAAAVAMAATLTQQQQPATGP QPSLGVSFGTPFGSGIGTGLQSSGLGSSNLGGFGTSSGFGCSTTGASTFGFGTTNKPSGS LSAGFGSSSTSGFNFSNPGITASAGLTFGVSNPASAGFGTGGQLLQLKKPPAGNKRGKR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pCellFree_c-mcherry_5800
Synonyms:
''
Description:
fluorescent marker, with tag/fusion/marker assay, Gateway® clone, bacterial expression expression with T7 forward and T7 promoter and attR1 and ccdB and CmR and cat_promoter-var002 and attR2 and mCherry and 8xHis and pBADrev and T7 Terminator and lac operator and lac promoter and CAP binding site and ori and AmpR and AmpR promoter; ampicillin resistance in bacterial;
Comments:
None
Size (bp):
5266
Parent Vector:
None
Properties:
Gateway, bacterial expression, fluorescent marker, with tag/fusion/marker
Author Name:
Shayli
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial operon
lac operator
var003
3137
3159
bacterial origin
ori
var012
3517
4105
fluorescent marker
mCherry
var007
2037
2735
negative selection marker
ccdB
var004
331
636
primer
T7 forward
null
7
26
primer
pBADrev
null
2964
2983
promoter
T7 promoter
null
7
25
promoter
cat_promoter-var002
null
1638
1740
promoter
lac promoter
var001
3163
3193
promoter
AmpR promoter
var003
5137
5241
protein binding site
CAP binding site
var002
3208
3229
recombination site
attR1
var002
160
284
recombination site
attR2
var003
1887
2011
selectable marker
CmR
var001
956
1637
selectable marker
AmpR
var016
4276
5136
tag
8xHis
var007
2744
2765
trxn termination sequence
T7 Terminator
var002
2967
2984
