Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pIEx-nEGFP-DC
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00964828
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1410nts
Open reading frame: 23 to 1432
GTACAAAAAAGCAGGCTCCACCATGGAGGAAAGAATGGAAATGATTTCTGAAAGGCCAAA
AGAGAGTATGTATTCCTGGAACAAAACTGCAGAGAAAAGTGATTTTGAAGCTGTAGAAGC
ACTTATGTCAATGAGCTGCAGTTGGAAGTCTGATTTTAAGAAATACGTTGAAAACAGACC
TGTTACACCAGTATCTGATTTGTCAGAGGAAGAGAATCTGCTTCCGGGAACACCTGATTT
TCATACAATCCCAGCATTTTGTTTGACTCCACCTTACAGTCCTTCTGACTTTGAACCCTC
TCAAGTGTCAAATCTGATGGCACCAGCGCCATCTACTGTACACTTCAAGTCACTCTCAGA
TACTGCCAAACCTCACATTGCCGCACCTTTCAAAGAGGAAGAAAAGAGCCCAGTATCTGC
CCCCAAACTCCCCAAAGCTCAGGCAACAAGTGTGATTCGTCATACAGCTGATGCCCAGCT
ATGTAACCACCAGACCTGCCCAATGAAAGCAGCCAGCATCCTCAACTATCAGAACAATTC
TTTTAGAAGAAGAACCCACCTAAATGTTGAGGCTGCAAGAAAGAACATACCATGTGCCGC
TGTGTCACCAAACAGATCCAAATGTGAGAGAAACACAGTGGCAGATGTTGATGAGAAAGC
AAGTGCTGCACTTTATGACTTTTCTGTGCCTTCCTCAGAGACGGTCATCTGCAGGTCTCA
GCCAGCCCCTGTGTCCCCACAACAGAAGTCAGTGTTGGTCTCTCCACCTGCAGTATCTGC
AGGGGGAGTGCCACCTATGCCGGTCATCTGCCAGATGGTTCCCCTTCCTGCCAACAACCC
TGTTGTGACAACAGTCGTTCCCAGCACTCCTCCCAGCCAGCCACCAGCCGTTTGCCCCCC
TGTTGTGTTCATGGGCACACAAGTCCCCAAAGGCGCTGTCATGTTTGTGGTACCCCAGCC
CGTTGTGCAGAGTTCAAAGCCTCCGGTGGTGAGCCCGAATGGCACCAGACTCTCTCCCAT
TGCCCCTGCTCCTGGGTTTTCCCCTTCAGCAGCAAAAGTCACTCCTCAGATTGATTCATC
AAGGATAAGGAGTCACATCTGTAGCCACCCAGGATGTGGCAAGACATACTTTAAAAGTTC
CCATCTGAAGGCCCACACGAGGACGCACACAGGAGAAAAGCCTTTCAGCTGTAGCTGGAA
AGGTTGTGAAAGGAGGTTTGCCCGTTCTGATGAACTGTCCAGACACAGGCGAACCCACAC
GGGTGAGAAGAAATTTGCGTGCCCCATGTGTGACCGGCGGTTCATGAGGAGTGACCATTT
GACCAAGCATGCCCGGCGCCATCTATCAGCCAAGAAGCTACCAAACTGGCAGATGGAAGT
GAGCAAGCTAAATGACATTGCTCTACCTCCAACCCCTGCTCCCACACAGTGAATCCACCC
AGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MEERMEMISERPKESMYSWNKTAEKSDFEAVEALMSMSCSWKSDFKKYVENRPVTPVSDL SEEENLLPGTPDFHTIPAFCLTPPYSPSDFEPSQVSNLMAPAPSTVHFKSLSDTAKPHIA APFKEEEKSPVSAPKLPKAQATSVIRHTADAQLCNHQTCPMKAASILNYQNNSFRRRTHL NVEAARKNIPCAAVSPNRSKCERNTVADVDEKASAALYDFSVPSSETVICRSQPAPVSPQ QKSVLVSPPAVSAGGVPPMPVICQMVPLPANNPVVTTVVPSTPPSQPPAVCPPVVFMGTQ VPKGAVMFVVPQPVVQSSKPPVVSPNGTRLSPIAPAPGFSPSAAKVTPQIDSSRIRSHIC SHPGCGKTYFKSSHLKAHTRTHTGEKPFSCSWKGCERRFARSDELSRHRRTHTGEKKFAC PMCDRRFMRSDHLTKHARRHLSAKKLPNWQMEVSKLNDIALPPTPAPTQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: insect cells Marker: ampicillin
Host Type: insect cells Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pIEx-nEGFP-DC
Synonyms:
''
Sequencing Primer:
Forward:
piex-seq-f
Reverse:
piex-seq-r
Description:
BAC mutagenesis, baculovirus/insect cell transposition assay, Gateway® recombinational cloning clone, baculovirus/insect cell expression expression with baculovirus_recombination_region_(lef2_ORF603) and hr5 and IE1 and pIEx_seq_F and p10 promoter and EGFP and EGFP_N and YFP_Primer and EGFP_C and attR1 and lac UV5 promoter and Chloramphenicol-R and ccdB and attR2 and ie1 terminator and pIEx_seq_R and baculovirus_recombination_region_(ORF1629) and Origin and AmpR and AmpR promoter and f1; ampicillin resistance in bacterial, ampicillin resistance in insect cells;
Comments:
CPD only
Size (bp):
9076
Parent Vector:
None
Properties:
BAC mutagenesis, Gateway, baculovirus/insect cell expression, baculovirus/insect cell transposition, recombinational cloning
Author Name:
Joshua LaBaer
Justin Saul
Yanyang
Justin Saul
Yanyang
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
flourescent marker
EGFP
null
2394
3111
flourescent marker
EGFP_N
null
2439
2460
flourescent marker
EGFP_C
null
3047
3068
origin
Origin
null
6800
7388
origin
f1
null
8551
9006
primer
pIEx_seq_F
null
2250
2269
primer
YFP_Primer
null
2876
2895
primer
pIEx_seq_R
null
4901
4920
promoter
IE1
null
1682
2273
promoter
p10 promoter
null
2283
2392
promoter
lac UV5 promoter
null
3275
3305
promoter
AmpR promoter
null
8420
8524
recombination site
baculovirus_recombination_region_(lef2_ORF603)
null
329
1156
recombination site
attR1
null
3126
3250
recombination site
attR2
null
4706
4830
recombination site
baculovirus_recombination_region_(ORF1629)
null
5180
6537
selectable marker
Chloramphenicol-R
null
3359
4018
selectable marker
ccdB
null
4360
4665
selectable marker
AmpR
null
7559
8419
trln enhancer
hr5
null
1196
1678
trln terminator
ie1 terminator
null
4845
5151
