Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pIEx-cHis-DC
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
Mutation AA: a48t;g99a;c129a;c132t;g135t;g138t;c144t;g159t;c216a;g285c;c294t;g438c;g480a;g513c;g519c;t576c;g597a;t681c;g717a;t786c;c864t;c909g;c931t;g981a;c1014g;c1029g;c1104a;t1140g;c1198a;c1201a;t1206g;g1215a;g1230c;g1317c;c1365t;c1395g;a1410c;c1455t;c1491g;g1530c;t1551a;t1611c;t1614a;g1695t;c1704t;g1815a;g1818c;a1825t;g1826c;c1873t;c1875g;a1905g;c1947a;c2025t;c2031t;g2040c;g2091t;c2115t;c2145t;c2172a;c2190t;c2253t;g2283c;t2298c;g2337a;c2355a;c2412t;t2535g;g2604c;g2640a;c2670t;g2679t;g2688t;g2700a;g2703a;g2706a;c2722a;c2728t;g2751a;c2775a;g2778a;c2781t;g2787a;c2814t;g2829a;c2851t;g2853a;c2859a;g2892a;c2913t;c2919t;c2967a;g3000a;c3021t;g3027t;c3159t;c3162t;c3174t;c3195t;c3210g. Codon optimized. Mutation AA: a3222c;g3243a;g3318t;a3327g;c3342t;a3348t;c3357a;c3360t;c3366a;a3375t;c3396t;c3402a;c3417t;g3456t;c3460t
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00964890
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3519nts
Open reading frame: 1 to 3519
ATGGTCACAGTGGGGACCAATATCCTGCCCTCCAGACCCGCTGCTTCTGCCAATACAGCA
AGGGAAGACGCAGCTCTGTTTTCTAGAAGGATCCCACCAAGGCATAAAAACGGCGCAGCC
CAGCCTGGAGCTGCTCCTGGTCCTGGAGCCCCGGGCGCTAACATGGGGAATGCGCACTCC
AAAAGCGGGGACAGGCACAGCGCGCTCCCCGGCCGACCCGAGCTGTCTTTTTACGGCTCT
TTCCCCAGGAAATGGAGCGAGAACGTCTTCTTGGATAACGAGCTCCTGACCTCTAAGATC
CTGTCTGTGCTGCGGCCGCAGTCGGAGCGGGGCTTCCGCGCCGGGGACCTCCGCTACCCG
ACCCACTTTCTCAGCACCAACTCTGTCCTTGCCTCTGTCACAGCCTCGCTCAAGGAGCAC
CCCCGGGGCACCCTCCTCTCCGATGGCAGCCCGGCCCTGTCCAGGAATGTCGGTATGACA
GTCTCTCAGAAAGGGGGTCCCCAGCCAACACCCAGCCCCGCTGGCCCTGGGACGCAACTC
GGACCAATCACAGGAGAGATGGATGAAGCCGATTCCGCGTTTTTAAAATTTAAGCAAACA
GCTGATGACTCTCTGTCCCTTACATCTCCAAACACCGAGTCCATTTTTGTAGAAGATCCC
TACACCGCCTCGCTGAGGAGCGAGATTGAGTCAGACGGCCACGAGTTTGAAGCTGAATCC
TGGAGCCTCGCCGTGGATGCAGCCTACGCCAAGAAGCAAAAGAGGGAGGTGGTGAAAAGA
CAAGACGTCCTTTATGAGCTGATGCAGACAGAGGTGCACCACGTGCGGACGCTCAAGATC
ATGCTGAAGGTGTACTCCAGGGCTCTGCAGGAGGAGCTGCAGTTCAGCAGCAAGGCCATT
GGCCGCCTGTTCCCATGCGCTGACGACCTGTTGGAGACGCACAGCCACTTCCTCGCTCGG
CTCAAGGAGCGCCGCCAGGAATCCCTGGAGGAGGGCAGTGACCGGAATTATGTGATCCAG
AAAATCGGGGACCTCCTGGTTCAGCAGTTTTCAGGTGAAAATGGGGAGAGAATGAAAGAA
AAGTACGGTGTGTTTTGTAGTGGACACAATGAAGCTGTTAGTCATTACAAGTTGCTGCTG
CAGCAAAACAAGAAATTTCAAAACTTGATCAAGAAAATTGGCAACTTCTCCATCGTGAGG
AGGCTGGGCGTGCAAGAGTGCATTCTCCTCGTTACACAACGCATAACCAAATACCCAGTG
CTGGTGGAGCGCATCATCCAGAACACGGAAGCTGGCACTGAGGACTATGAAGACCTCACC
CAGGCCTTGAACCTCATCAAAGATATCATCTCACAAGTGGACGCTAAGGTCAGTGAGTGT
GAGAAGGGCCAGCGGCTCAGGGAGATCGCCGGGAAGATGGACCTGAAGTCTTCCAGCAAA
CTCAAGAACGGGCTTACCTTCCGCAAGGAAGACATGCTTCAGCGGCAGCTGCACCTGGAG
GGCATGCTATGCTGGAAGACCACATCAGGCCGCTTGAAAGATATCCTGGCAATCCTGCTG
ACCGACGTACTTTTGCTGCTACAAGAAAAAGATCAGAAATACGTCTTTGCCTCAGTGGAC
TCAAAGCCACCCGTCATCTCGTTACAAAAGCTCATCGTGAGGGAAGTGGCCAACGAGGAG
AAAGCGATGTTTCTTATCAGCGCTTCCTTGCAAGGGCCGGAGATGTATGAAATCTACACG
AGCTCCAAAGAGGACAGGAACGCCTGGATGGCCCACATCCAAAGGGCTGTGGAGAGCTGC
CCTGACGAGGAGGAAGGCCCCTTCTCCCTGCCCGAAGAGGAAAGGAAGGTGGTCGAGGCC
CGCGCCACGAGATTGCGGGACTTTCAAGAGCGGTTGAGCATGAAGGACCAGCTGATCGCA
CAGAGCCTCCTAGAGAAACAGCAGATATACCTGGAGATGGCCGAGATGGGCGGCCTCGAA
GACCTGCCCCAGCCCCGAGGCCTATTCCGTGGAGGGGACCCATCTGAGACTCTGCAGGGC
GAGCTAATTCTCAAGTCGGCCATGAGCGAGATCGAGGGCATCCAGAGCCTTATCTGCAGG
CAGCTGGGCAGCGCTAACGGCCAGGCGGAAGACGGAGGCAGCTCTACAGGCCCGCCCAGG
AGGGCTGAGACATTCGCGGGCTACGACTGTACAAACAGCCCCACCAAGAATGGCAGTTTC
AAGAAGAAAGTCAGCAGCACTGACCCCAGGCCTCGAGACTGGCGAGGCCCCCCAAACAGC
CCCGACTTGAAGCTCAGCGACAGTGACATTCCTGGGAGCTCTGAGGAATCGCCGCAAGTG
GTGGAGGCGCCAGGAACGGAATCCGATCCCCGTCTGCCCACCGTCCTGGAGTCGGAGCTT
GTCCAGCGGATTCAGACACTGTCCCAGCTGCTCCTGAACCTTCAGGCGGTAATCGCCCAC
CAGGACAGCTATGTGGAGACGCAGCGGGCTGCCATCCAGGAGCGGGAGAAGCAGTTCCGG
CTGCAGTCGACGCGGGGGAACCTGCTGCTGGAGCAGGAGCGGCAACGCAACTTCGAGAAG
CAGCGGGAGGAGCGCGCGGCCCTCGAGAAGCTGCAGAGCCAGCTGCGGCACGAGCAGCAA
CGCTGGGAGCGCGAGCGCCAGTGGCAGCATCAGGAGCTTGAGCGTGCTGGCGCGCGGCTA
CAAGAACGCGAGGGCGAGGCGAGGCAGTTACGCGAGCGGCTGGAGCAGGAACGGGCCGAG
CTGGAGCGCCAGCGACAAGCTTACCAACACGACCTGGAGCGGCTGCGCGAGGCTCAGCGT
GCCGTGGAACGCGAGCGGGAGCGCCTGGAGTTACTGCGACGCCTCAAGAAGCAGAACACC
GCGCCAGGCGCACTGCCGCCCGACACACTGGCTGAGGCTCAGCCCCCAAGCCACCCTCCC
AGCTTCAACGGGGAAGGGCTGGAGGGACCTCGTGTGAGCATGCTGCCATCCGGCGTGGGA
CCAGAGTACGCAGAGCGCCCTGAGGTTGCTCGCCGGGACAGCGCCCCCACCGAGAACCGG
CTGGCCAAGAGCGATGTGCCCATCCAGCTGCTCAGCGCCACCAACCAGTTCCAGAGGCAG
GCGGCCGTGCAGCAGCAGATCCCCACCAAGCTGGCGGCTTCTACCAAGGGTGGTAAGGAC
AAGGGCGGCAAGAGTAGGGGCTCTCAGCGGTGGGAGAGCTCCGCGTCCTTCGACCTGAAG
CAACAGCTGCTGCTCAACAAGCTCATGGGGAAAGATGAGAGCACCTCACGGAACCGCCGC
TCGCTGAGCCCTATCCTTCCCGGCAGGCACAGTCCTGCGCCTCCACCTGACCCTGGATTT
CCCGCACCGAGCCCTCCGCCAGCTGACAGCCCCTCTGAGGGATTCTCTCTCAAGGCTGGG
GGCACAGCCCTCCTGCCCGGGCCCCCAGCTCCCTCTCCATTGCCGGCCACACCACTCAGC
GCCAAGGAGGACGCCAGCAAAGAAGACGTCATCTTCTTC
Protein sequence predicted based on nucleotide sequence
MVTVGTNILPSRPAASANTAREDAALFSRRIPPRHKNGAAQPGAAPGPGAPGANMGNAHS KSGDRHSALPGRPELSFYGSFPRKWSENVFLDNELLTSKILSVLRPQSERGFRAGDLRYP THFLSTNSVLASVTASLKEHPRGTLLSDGSPALSRNVGMTVSQKGGPQPTPSPAGPGTQL GPITGEMDEADSAFLKFKQTADDSLSLTSPNTESIFVEDPYTASLRSEIESDGHEFEAES WSLAVDAAYAKKQKREVVKRQDVLYELMQTEVHHVRTLKIMLKVYSRALQEELQFSSKAI GRLFPCADDLLETHSHFLARLKERRQESLEEGSDRNYVIQKIGDLLVQQFSGENGERMKE KYGVFCSGHNEAVSHYKLLLQQNKKFQNLIKKIGNFSIVRRLGVQECILLVTQRITKYPV LVERIIQNTEAGTEDYEDLTQALNLIKDIISQVDAKVSECEKGQRLREIAGKMDLKSSSK LKNGLTFRKEDMLQRQLHLEGMLCWKTTSGRLKDILAILLTDVLLLLQEKDQKYVFASVD SKPPVISLQKLIVREVANEEKAMFLISASLQGPEMYEIYTSSKEDRNAWMAHIQRAVESC PDEEEGPFSLPEEERKVVEARATRLRDFQERLSMKDQLIAQSLLEKQQIYLEMAEMGGLE DLPQPRGLFRGGDPSETLQGELILKSAMSEIEGIQSLICRQLGSANGQAEDGGSSTGPPR RAETFAGYDCTNSPTKNGSFKKKVSSTDPRPRDWRGPPNSPDLKLSDSDIPGSSEESPQV VEAPGTESDPRLPTVLESELVQRIQTLSQLLLNLQAVIAHQDSYVETQRAAIQEREKQFR LQSTRGNLLLEQERQRNFEKQREERAALEKLQSQLRHEQQRWERERQWQHQELERAGARL QEREGEARQLRERLEQERAELERQRQAYQHDLERLREAQRAVERERERLELLRRLKKQNT APGALPPDTLAEAQPPSHPPSFNGEGLEGPRVSMLPSGVGPEYAERPEVARRDSAPTENR LAKSDVPIQLLSATNQFQRQAAVQQQIPTKLAASTKGGKDKGGKSRGSQRWESSASFDLK QQLLLNKLMGKDESTSRNRRSLSPILPGRHSPAPPPDPGFPAPSPPPADSPSEGFSLKAG GTALLPGPPAPSPLPATPLSAKEDASKEDVIFF
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: insect cells Marker: ampicillin
Host Type: insect cells Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pIEx-cHis-DC
Synonyms:
''
Sequencing Primer:
Forward:
pIEx_seq_F
Reverse:
pIEx_seq_R
Description:
BAC mutagenesis, baculovirus/insect cell transposition assay, Gateway® recombinational cloning clone, baculovirus/insect cell expression expression with baculovirus_recombination_region_(lef2_ORF603) and hr5 and IE1 and pIEx_seq_F and p10 promoter and attR1 and lac UV5 promoter and Chloramphenicol-R and ccdB and attR2 and TEV and ie1 terminator and pIEx_seq_R and baculovirus_recombination_region_(ORF1629) and Origin and AmpR and AmpR promoter and f1; ampicillin resistance in bacterial, ampicillin resistance in insect cells;
Comments:
CPD only
Size (bp):
8444
Parent Vector:
None
Properties:
BAC mutagenesis, Gateway, baculovirus/insect cell expression, baculovirus/insect cell transposition, recombinational cloning
Author Name:
Joshua LaBaer
Justin Saul
Yanyang
Justin Saul
Yanyang
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
origin
Origin
null
6168
6756
origin
f1
null
7919
8374
primer
pIEx_seq_F
null
2250
2269
primer
pIEx_seq_R
null
4269
4288
promoter
IE1
null
1682
2273
promoter
p10 promoter
null
2283
2392
promoter
lac UV5 promoter
null
2551
2581
promoter
AmpR promoter
null
7788
7892
protease cleavage site
TEV
null
4129
4149
recombination site
baculovirus_recombination_region_(lef2_ORF603)
null
329
1156
recombination site
attR1
null
2402
2526
recombination site
attR2
null
3982
4106
recombination site
baculovirus_recombination_region_(ORF1629)
null
4548
5905
selectable marker
Chloramphenicol-R
null
2635
3294
selectable marker
ccdB
null
3636
3941
selectable marker
AmpR
null
6927
7787
trln enhancer
hr5
null
1196
1678
trln terminator
ie1 terminator
null
4213
4519
