Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pcDNA3.1-3xFLAG-V5-ccdB
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Economos NG
Thapar U
Balasubramanian N
Karras GI
Glazer PM
Thapar U
Balasubramanian N
Karras GI
Glazer PM
HsCD00970529
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 7905nts
Open reading frame: 1 to 7905
ATGATCCCCGCCCTGCGGGAGCTGGGCAGTGCCACACCAGAGGAATATAATACAGTTGTA
CAGAAGCCAAGACAAATTCTGTGTCAATTCATTGACCGGATACTTACAGATGTAAATGTT
GTTGCTGTAGAACTTGTAAAGAAAACTGACTCTCAGCCAACCTCCGTGATGTTGCTTGAT
TTCATCCAGCATATCATGAAATCCTCCCCACTTATGTTTGTAAATGTGAGTGGAAGCCAT
GAGCGCAAAGGCAGTTGTATTGAATTCAGTAATTGGATCATAACGAGACTTCTGCGGATT
GCAGCAACTCCCTCCTGTCATTTGTTACACAAGAAAATCTGTGAAGTCATCTGTTCATTA
TTATTTCTTTTTAAAAGCAAGAGTCCTGCTATTTTTGGGGTACTCACAAAAGAATTATTA
CAACTTTTTGAAGACTTGGTTTACCTCCATAGAAGAAATGTGATGGGTCATGCTGTGGAA
TGGCCAGTGGTCATGAGCCGATTTTTAAGTCAATTAGATGAACACATGGGATATTTACAA
TCAGCTCCTTTGCAGTTGATGAGTATGCAAAATTTAGAATTTATTGAAGTCACTTTATTA
ATGGTTCTTACTCGTATTATTGCAATTGTGTTTTTTAGAAGGCAAGAACTCTTACTTTGG
CAGATAGGTTGTGTTCTGCTAGAGTATGGTAGTCCAAAAATTAAATCCCTAGCAATTAGC
TTTTTAACAGAACTTTTTCAGCTTGGAGGACTACCAGCACAACCAGCTAGCACTTTTTTC
AGCTCATTTTTGGAATTATTAAAACACCTTGTAGAAATGGATACTGACCAATTGAAACTC
TATGAAGAGCCATTATCAAAGCTGATAAAGACACTATTTCCCTTTGAAGCAGAAGCTTAT
AGAAATATTGAACCTGTCTATTTAAATATGCTGCTGGAAAAACTCTGTGTCATGTTTGAA
GACGGTGTGCTCATGCGGCTTAAGTCTGATTTGCTAAAAGCAGCTTTGTGCCATTTACTG
CAGTATTTCCTTAAATTTGTGCCAGCTGGGTATGAATCTGCTTTACAAGTCAGGAAGGTC
TATGTGAGAAATATTTGTAAAGCTCTTTTGGATGTGCTTGGAATTGAGGTAGATGCAGAG
TACTTGTTGGGCCCACTTTATGCAGCTTTGAAAATGGAAAGTATGGAAATCATTGAGGAG
ATTCAATGCCAAACTCAACAGGAAAACCTCAGCAGTAATAGTGATGGAATATCACCCAAA
AGGCGTCGTCTCAGCTCGTCTCTAAACCCTTCTAAAAGAGCACCAAAACAGACTGAGGAA
ATTAAACATGTGGACATGAACCAAAAGAGCATATTATGGAGTGCACTGAAACAGAAAGCT
GAATCCCTTCAGATTTCCCTTGAATACAGTGGCCTAAAGAATCCTGTTATTGAGATGTTA
GAAGGAATTGCTGTTGTCTTACAACTGACTGCTCTGTGTACTGTTCATTGTTCTCATCAA
AACATGAACTGCCGTACTTTCAAGGACTGTCAACATAAATCCAAGAAGAAACCTTCTGTA
GTGATAACTTGGATGTCATTGGATTTTTACACAAAAGTGCTTAAGAGCTGTAGAAGTTTG
TTAGAATCTGTTCAGAAACTGGACCTGGAGGCAACCATTGATAAGGTGGTGAAAATTTAT
GATGCTTTGATTTATATGCAAGTAAACAGTTCATTTGAAGATCATATCCTGGAAGATTTA
TGTGGTATGCTCTCACTTCCATGGATTTATTCCCATTCTGATGATGGCTGTTTAAAGTTG
ACCACATTTGCCGCTAATCTTCTAACATTAAGCTGTAGGATTTCAGATAGCTATTCACCA
CAGGCACAATCACGATGTGTGTTTCTTCTGACTCTGTTTCCAAGAAGAATATTCCTTGAG
TGGAGAACAGCAGTTTACAACTGGGCCCTGCAGAGCTCCCATGAAGTAATCCGGGCTAGT
TGTGTTAGTGGATTTTTTATCTTATTGCAGCAGCAGAATTCTTGTAACAGAGTTCCCAAG
ATTCTTATAGATAAAGTCAAAGATGATTCTGACATTGTCAAGAAAGAATTTGCTTCTATA
CTTGGTCAACTTGTCTGTACTCTTCACGGCATGTTTTATCTGACAAGTTCTTTAACAGAA
CCTTTCTCTGAACACGGACATGTGGACCTCTTCTGTAGGAACTTGAAAGCCACTTCTCAA
CATGAATGTTCATCTTCTCAACTAAAAGCTTCTGTCTGCAAGCCATTCCTTTTCCTACTG
AAAAAAAAAATACCTAGTCCAGTAAAACTTGCTTTCATAGATAATCTACATCATCTTTGT
AAGCATCTTGATTTTAGAGAAGATGAAACAGATGTAAAAGCAGTTCTTGGAACTTTATTA
AATTTAATGGAAGATCCAGACAAAGATGTTAGAGTGGCTTTTAGTGGAAATATCAAGCAC
ATATTGGAATCCTTGGACTCTGAAGATGGATTTATAAAGGAGCTTTTTGTCTTAAGAATG
AAGGAAGCATATACACATGCCCAAATATCAAGAAATAATGAGCTGAAGGATACCTTGATT
CTTACAACAGGGGATATTGGAAGGGCCGCAAAAGGAGATTTGGTACCATTTGCACTCTTA
CACTTATTGCATTGTTTGTTATCCAAGTCAGCATCTGTCTCTGGAGCAGCATACACAGAA
ATTAGAGCTCTGGTTGCAGCTAAAAGTGTTAAACTGCAAAGTTTTTTCAGCCAGTATAAG
AAACCCATCTGTCAGTTTTTGGTAGAATCCCTTCACTCTAGTCAGATGACAGCACTTCCG
AATACTCCATGCCAGAATGCTGACGTGCGAAAACAAGATGTGGCTCACCAGAGAGAAATG
GCTTTAAATACGTTGTCTGAAATTGCCAACGTTTTCGACTTTCCTGATCTTAATCGTTTT
CTTACTAGGACATTACAAGTTCTACTACCTGATCTTGCTGCCAAAGCAAGCCCTGCAGCT
TCTGCTCTCATTCGAACTTTAGGAAAACAATTAAATGTCAATCGTAGAGAGATTTTAATA
AACAACTTCAAATATATTTTTTCTCATTTGGTCTGTTCTTGTTCCAAAGATGAATTAGAA
CGTGCCCTTCATTATCTGAAGAATGAAACAGAAATTGAACTGGGGAGCCTGTTGAGACAA
GATTTCCAAGGATTGCATAATGAATTATTGCTGCGTATTGGAGAACACTATCAACAGGTT
TTTAATGGTTTGTCAATACTTGCCTCATTTGCATCCAGTGATGATCCATATCAGGGCCCG
AGAGATATCATATCACCTGAACTGATGGCTGATTATTTACAACCCAAATTGTTGGGCATT
TTGGCTTTTTTTAACATGCAGTTACTGAGCTCTAGTGTTGGCATTGAAGATAAGAAAATG
GCCTTGAACAGTTTGATGTCTTTGATGAAGTTAATGGGACCCAAACATGTCAGTTCTGTG
AGGGTGAAGATGATGACCACACTGAGAACTGGCCTTCGATTCAAGGATGATTTTCCTGAA
TTGTGTTGCAGAGCTTGGGACTGCTTTGTTCGCTGCCTGGATCATGCTTGTCTGGGCTCC
CTTCTCAGTCATGTAATAGTAGCTTTGTTACCTCTTATACACATCCAGCCTAAAGAAACT
GCAGCTATCTTCCACTACCTCATAATTGAAAACAGGGATGCTGTGCAAGATTTTCTTCAT
GAAATATATTTTTTACCTGATCATCCAGAATTAAAAAAGATAAAAGCCGTTCTCCAGGAA
TACAGAAAGGAGACCTCTGAGAGCACTGATCTTCAGACAACTCTTCAGCTCTCTATGAAG
GCCATTCAACATGAAAATGTCGATGTTCGTATTCATGCTCTTACAAGCTTGAAGGAAACC
TTGTATAAAAATCAGGAAAAACTGATAAAGTATGCAACAGACAGTGAAACAGTAGAACCT
ATTATCTCACAGTTGGTGACAGTGCTTTTGAAAGGTTGCCAAGATGCAAACTCTCAAGCT
CGGTTGCTCTGTGGGGAATGTTTAGGGGAATTGGGGGCGATAGATCCAGGTCGATTAGAT
TTCTCAACAACTGAAACTCAAGGAAAAGATTTTACATTTGTGACTGGAGTAGAAGATTCA
AGCTTTGCCTATGGATTATTGATGGAGCTAACAAGAGCTTACCTTGCGTATGCTGATAAT
AGCCGAGCTCAAGATTCAGCTGCCTATGCCATTCAGGAGTTGCTTTCTATTTATGACTGT
AGAGAGATGGAGACCAACGGCCCAGGTCACCAATTGTGGAGGAGATTTCCTGAGCATGTT
CGGGAAATACTAGAACCTCATCTAAATACCAGATACAAGAGTTCTCAGAAGTCAACCGAT
TGGTCTGGAGTAAAGAAGCCAATTTACTTAAGTAAATTGGGTAGTAACTTTGCAGAATGG
TCAGCATCTTGGGCAGGTTATCTTATTACAAAGGTTCGACATGATCTTGCCAGTAAAATT
TTCACCTGCTGTAGCATTATGATGAAGCATGATTTCAAAGTGACCATCTATCTTCTTCCA
CATATTCTGGTGTATGTCTTACTGGGTTGTAATCAAGAAGATCAGCAGGAGGTTTATGCA
GAAATTATGGCAGTTCTAAAGCATGACGATCAGCATACCATAAATACCCAAGACATTGCA
TCTGATCTGTGTCAACTCAGTACACAGACTGTGTTCTCCATGCTTGACCATCTCACACAG
TGGGCAAGGCACAAATTTCAGGCACTGAAAGCTGAGAAATGTCCACACAGCAAATCAAAC
AGAAATAAGGTAGACTCAATGGTATCTACTGTGGATTATGAAGACTATCAGAGTGTAACC
CGTTTTCTAGACCTCATACCCCAGGATACTCTGGCAGTAGCTTCCTTTCGCTCCAAAGCA
TACACACGAGCTGTAATGCACTTTGAATCATTTATTACAGAAAAGAAGCAAAATATTCAG
GAACATCTTGGATTTTTACAGAAATTGTATGCTGCTATGCATGAACCTGATGGAGTGGCC
GGAGTCAGTGCAATTAGAAAGGCAGAACCATCTCTAAAAGAACAGATCCTTGAACATGAA
AGCCTTGGCTTGCTGAGGGATGCCACTGCTTGTTATGACAGGGCTATTCAGCTAGAACCA
GACCAGATCATTCATTATCATGGTGTAGTAAAGTCCATGTTAGGTCTTGGTCAGCTGTCT
ACTGTTATCACTCAGGTGAATGGAGTGCATGCTAACAGGTCCGAGTGGACAGATGAATTA
AACACGTACAGAGTGGAAGCAGCTTGGAAATTGTCACAGTGGGATTTGGTGGAAAACTAT
TTGGCAGCAGATGGAAAATCTACAACATGGAGTGTCAGACTGGGACAGCTATTATTATCA
GCCAAAAAAAGAGATATCACAGCTTTTTATGACTCACTGAAACTAGTGAGAGCAGAACAA
ATTGTACCTCTTTCAGCTGCAAGCTTTGAAAGAGGCTCCTACCAACGAGGATATGAATAT
ATTGTGAGATTGCACATGTTATGTGAGTTGGAGCATAGCATCAAACCACTTTTCCAGCAT
TCTCCAGGTGACAGTTCTCAAGAAGATTCTCTAAACTGGGTAGCTCGACTAGAAATGACC
CAGAATTCCTACAGAGCCAAGGAGCCTATCCTGGCTCTCCGGAGGGCTTTACTAAGCCTC
AACAAAAGACCAGATTACAATGAAATGGTTGGAGAATGCTGGCTGCAGAGTGCCAGGGTA
GCTAGAAAGGCTGGTCACCACCAGACAGCCTACAATGCTCTCCTTAATGCAGGGGAATCA
CGACTCGCTGAACTGTACGTGGAAAGGGCAAAGTGGCTCTGGTCCAAGGGTGATGTTCAC
CAGGCACTAATTGTTCTTCAAAAAGGTGTTGAATTATGTTTTCCTGAAAATGAAACCCCA
CCTGAGGGTAAGAACATGTTAATCCATGGTCGAGCTATGCTACTAGTGGGCCGATTTATG
GAAGAAACAGCTAACTTTGAAAGCAATGCAATTATGAAAAAATATAAGGATGTGACCGCG
TGCCTGCCAGAATGGGAGGATGGGCATTTTTACCTTGCCAAGTACTATGACAAATTGATG
CCCATGGTCACAGACAACAAAATGGAAAAGCAAGGTGATCTCATCCGGTATATAGTTCTT
CATTTTGGCAGATCTCTACAATATGGAAATCAGTTCATATATCAGTCAATGCCACGAATG
TTAACTCTATGGCTTGATTATGGTACAAAGGCATATGAATGGGAAAAAGCTGGCCGCTCC
GATCGTGTACAAATGAGGAATGATTTGGGTAAAATAAACAAGGTTATCACAGAGCATACA
AACTATTTAGCTCCATATCAATTTTTGACTGCTTTTTCACAATTGATCTCTCGAATTTGT
CATTCTCACGATGAAGTTTTTGTTGTCTTGATGGAAATAATAGCCAAAGTATTTCTAGCC
TATCCTCAACAAGCAATGTGGATGATGACAGCTGTGTCAAAGTCATCTTATCCCATGCGT
GTGAACAGATGCAAGGAAATCCTCAATAAAGCTATTCATATGAAAAAATCCTTAGAGAAG
TTTGTTGGAGATGCAACTCGCCTAACAGATAAGCTTCTAGAATTGTGCAATAAACCGGTT
GATGGAAGTAGTTCCACATTAAGCATGAGCACTCATTTTAAAATGCTTAAAAAGCTGGTA
GAAGAAGCAACATTTAGTGAAATCCTCATTCCTCTACAATCAGTCATGATACCTACACTT
CCATCAATTCTGGGTACCCATGCTAACCATGCTAGCCATGAACCATTTCCTGGACATTGG
GCCTATATTGCAGGGTTTGATGATATGGTGGAAATTCTTGCTTCTCTTCAGAAACCAAAG
AAGATTTCTTTAAAAGGCTCAGATGGAAAGTTCTACATCATGATGTGTAAGCCAAAAGAT
GACCTGAGAAAGGATTGTAGACTAATGGAATTCAATTCCTTGATTAATAAGTGCTTAAGA
AAAGATGCAGAGTCTCGTAGAAGAGAACTTCATATTCGAACATATGCAGTTATTCCACTA
AATGATGAATGTGGGATTATTGAATGGGTGAACAACACTGCTGGTTTGAGACCTATTCTG
ACCAAACTATATAAAGAAAAGGGAGTGTATATGACAGGAAAAGAACTTCGCCAGTGTATG
CTACCAAAGTCAGCAGCTTTATCTGAAAAACTCAAAGTATTCCGAGAATTTCTCCTGCCC
AGGCATCCTCCTATTTTTCATGAGTGGTTTCTGAGAACATTCCCTGATCCTACATCATGG
TACAGTAGTAGATCAGCTTACTGCCGTTCCACTGCAGTAATGTCAATGGTTGGTTATATT
CTGGGGCTTGGAGACCGTCATGGTGAAAATATTCTCTTTGATTCTTTGACTGGTGAATGC
GTACATGTAGATTTCAATTGTCTTTTCAATAAGGGAGAAACCTTTGAAGTTCCAGAAATT
GTGCCATTTCGCCTGACTCATAATATGGTTAATGGAATGGGTCCTATGGGAACAGAGGGT
CTTTTTCGAAGAGCATGTGAAGTTACAATGAGGCTGATGCGTGATCAGCGAGAGCCTTTA
ATGAGTGTCTTAAAGACTTTTCTACATGATCCTCTTGTGGAATGGAGTAAACCAGTGAAA
GGGCATTCCAAAGCGCCACTGAATGAAACTGGAGAAGTTGTCAATGAAAAGGCCAAGACC
CATGTTCTTGACATTGAGCAGCGACTACAAGGTGTAATCAAGACTCGAAATAGAGTGACA
GGACTGCCGTTATCTATTGAAGGACATGTGCATTACCTTATACAAGAAGCTACTGATGAA
AACTTACTATGCCAGATGTATCTTGGTTGGACTCCATATATGTGA
Protein sequence predicted based on nucleotide sequence
MIPALRELGSATPEEYNTVVQKPRQILCQFIDRILTDVNVVAVELVKKTDSQPTSVMLLD FIQHIMKSSPLMFVNVSGSHERKGSCIEFSNWIITRLLRIAATPSCHLLHKKICEVICSL LFLFKSKSPAIFGVLTKELLQLFEDLVYLHRRNVMGHAVEWPVVMSRFLSQLDEHMGYLQ SAPLQLMSMQNLEFIEVTLLMVLTRIIAIVFFRRQELLLWQIGCVLLEYGSPKIKSLAIS FLTELFQLGGLPAQPASTFFSSFLELLKHLVEMDTDQLKLYEEPLSKLIKTLFPFEAEAY RNIEPVYLNMLLEKLCVMFEDGVLMRLKSDLLKAALCHLLQYFLKFVPAGYESALQVRKV YVRNICKALLDVLGIEVDAEYLLGPLYAALKMESMEIIEEIQCQTQQENLSSNSDGISPK RRRLSSSLNPSKRAPKQTEEIKHVDMNQKSILWSALKQKAESLQISLEYSGLKNPVIEML EGIAVVLQLTALCTVHCSHQNMNCRTFKDCQHKSKKKPSVVITWMSLDFYTKVLKSCRSL LESVQKLDLEATIDKVVKIYDALIYMQVNSSFEDHILEDLCGMLSLPWIYSHSDDGCLKL TTFAANLLTLSCRISDSYSPQAQSRCVFLLTLFPRRIFLEWRTAVYNWALQSSHEVIRAS CVSGFFILLQQQNSCNRVPKILIDKVKDDSDIVKKEFASILGQLVCTLHGMFYLTSSLTE PFSEHGHVDLFCRNLKATSQHECSSSQLKASVCKPFLFLLKKKIPSPVKLAFIDNLHHLC KHLDFREDETDVKAVLGTLLNLMEDPDKDVRVAFSGNIKHILESLDSEDGFIKELFVLRM KEAYTHAQISRNNELKDTLILTTGDIGRAAKGDLVPFALLHLLHCLLSKSASVSGAAYTE IRALVAAKSVKLQSFFSQYKKPICQFLVESLHSSQMTALPNTPCQNADVRKQDVAHQREM ALNTLSEIANVFDFPDLNRFLTRTLQVLLPDLAAKASPAASALIRTLGKQLNVNRREILI NNFKYIFSHLVCSCSKDELERALHYLKNETEIELGSLLRQDFQGLHNELLLRIGEHYQQV FNGLSILASFASSDDPYQGPRDIISPELMADYLQPKLLGILAFFNMQLLSSSVGIEDKKM ALNSLMSLMKLMGPKHVSSVRVKMMTTLRTGLRFKDDFPELCCRAWDCFVRCLDHACLGS LLSHVIVALLPLIHIQPKETAAIFHYLIIENRDAVQDFLHEIYFLPDHPELKKIKAVLQE YRKETSESTDLQTTLQLSMKAIQHENVDVRIHALTSLKETLYKNQEKLIKYATDSETVEP IISQLVTVLLKGCQDANSQARLLCGECLGELGAIDPGRLDFSTTETQGKDFTFVTGVEDS SFAYGLLMELTRAYLAYADNSRAQDSAAYAIQELLSIYDCREMETNGPGHQLWRRFPEHV REILEPHLNTRYKSSQKSTDWSGVKKPIYLSKLGSNFAEWSASWAGYLITKVRHDLASKI FTCCSIMMKHDFKVTIYLLPHILVYVLLGCNQEDQQEVYAEIMAVLKHDDQHTINTQDIA SDLCQLSTQTVFSMLDHLTQWARHKFQALKAEKCPHSKSNRNKVDSMVSTVDYEDYQSVT RFLDLIPQDTLAVASFRSKAYTRAVMHFESFITEKKQNIQEHLGFLQKLYAAMHEPDGVA GVSAIRKAEPSLKEQILEHESLGLLRDATACYDRAIQLEPDQIIHYHGVVKSMLGLGQLS TVITQVNGVHANRSEWTDELNTYRVEAAWKLSQWDLVENYLAADGKSTTWSVRLGQLLLS AKKRDITAFYDSLKLVRAEQIVPLSAASFERGSYQRGYEYIVRLHMLCELEHSIKPLFQH SPGDSSQEDSLNWVARLEMTQNSYRAKEPILALRRALLSLNKRPDYNEMVGECWLQSARV ARKAGHHQTAYNALLNAGESRLAELYVERAKWLWSKGDVHQALIVLQKGVELCFPENETP PEGKNMLIHGRAMLLVGRFMEETANFESNAIMKKYKDVTACLPEWEDGHFYLAKYYDKLM PMVTDNKMEKQGDLIRYIVLHFGRSLQYGNQFIYQSMPRMLTLWLDYGTKAYEWEKAGRS DRVQMRNDLGKINKVITEHTNYLAPYQFLTAFSQLISRICHSHDEVFVVLMEIIAKVFLA YPQQAMWMMTAVSKSSYPMRVNRCKEILNKAIHMKKSLEKFVGDATRLTDKLLELCNKPV DGSSSTLSMSTHFKMLKKLVEEATFSEILIPLQSVMIPTLPSILGTHANHASHEPFPGHW AYIAGFDDMVEILASLQKPKKISLKGSDGKFYIMMCKPKDDLRKDCRLMEFNSLINKCLR KDAESRRRELHIRTYAVIPLNDECGIIEWVNNTAGLRPILTKLYKEKGVYMTGKELRQCM LPKSAALSEKLKVFREFLLPRHPPIFHEWFLRTFPDPTSWYSSRSAYCRSTAVMSMVGYI LGLGDRHGENILFDSLTGECVHVDFNCLFNKGETFEVPEIVPFRLTHNMVNGMGPMGTEG LFRRACEVTMRLMRDQREPLMSVLKTFLHDPLVEWSKPVKGHSKAPLNETGEVVNEKAKT HVLDIEQRLQGVIKTRNRVTGLPLSIEGHVHYLIQEATDENLLCQMYLGWTPYM*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pcDNA3.1-3xFLAG-V5-ccdB
Synonyms:
'
Sequencing Primer:
Forward:
CMV forward
Reverse:
Unknown
Description:
with tag/fusion/marker assay, Gateway®, donor (entry) clone, mammalian expression expression with CMV enhancer and CMV Promoter and 3xFlag and V5 and attR1 and lac UV5 promoter and CmR and ccdB and attR2 and BGH pA and f1 ori and SV40 promoter and SV40 Ori and HygR and SV40 poly(A) signal and M13 reverse and lac operator and lac promoter and CAP binding site and ori and AmpR and AmpR promoter; ampicillin resistance in bacterial;
Comments:
None
Size (bp):
7361
Parent Vector:
None
Properties:
Gateway, donor (entry), mammalian expression, with tag/fusion/marker
Author Name:
Economos NG
Thapar U
Balasubramanian N
Karras GI
Glazer PM
Thapar U
Balasubramanian N
Karras GI
Glazer PM
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
3xFlag
three tandem FLAGŪ epitope tags and an enterokinase cleavage site
908
973
CDS
V5
epitope tag from simian virus 5
992
1033
CDS
CmR
confers resistance to chloramphenicol
1264
1923
CDS
ccdB
bacterial toxin that poisons DNA gyrase
2265
2570
CDS
HygR
confers resistance to hygromycin
3882
4907
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
6365
7225
enhancer
CMV enhancer
human cytomegalovirus immediate early enhancer
235
614
poly-A signal
BGH pA
bovine growth hormone polyadenylation signal
2792
3016
poly-A signal
SV40 poly(A) signal
SV40 polyadenylation signal
5037
5158
primer_bind
M13 reverse
common sequencing primer
5207
5223
promoter
CMV Promoter
human cytomegalovirus immediate early promoter
615
818
promoter
lac UV5 promoter
E. coli lac promoter with an "up" mutation
1180
1210
promoter
SV40 promoter
SV40 enhancer and early promoter
3504
3833
promoter
lac promoter
promoter for the E. coli lac operon
5255
5285
promoter
AmpR promoter
null
7226
7330
protein binding site
attR1
recombination site for the Gateway LR reaction
1031
1155
protein binding site
attR2
recombination site for the Gateway LR reaction
2611
2735
protein binding site
lac operator
binding site for lac repressor to inhibit transcription in E. coli
5231
5247
protein binding site
CAP binding site
CAP binding activates transcription in the presence of cAMP.
5300
5321
rep_origin
f1 ori
f1 bacteriophage origin of replication
3062
3490
rep_origin
SV40 Ori
SV40 origin of replication
3684
3819
rep_origin
ori
origin of replication
5609
6194
