Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pcDNA3.1-3xFLAG-V5-ccdB
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Economos NG
Thapar U
Balasubramanian N
Karras GI
Glazer PM
Thapar U
Balasubramanian N
Karras GI
Glazer PM
HsCD00970560
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6069nts
Open reading frame: 1 to 6069
ATGAGCGGACGGCAAAGAACGCTTTTTCAGACGTGGGGCTCAAGTATCTCCCGATCATCT
GGGACTCCGGGTTGCAGCTCCGGAACTGAGCGACCTCAGAGCCCTGGCAGCTCCAAGGCG
CCTTTGCCAGCAGCAGCGGAGGCTCAGCTGGAGTCGGACGATGATGTGTTGCTTGTCGCG
GCGTACGAGGCTGAGCGGCAGTTGTGTCTAGAGAATGGCGGGTTCTGCACCTCCGCGGGC
GCCCTGTGGATTTACCCTACCAATTGCCCAGTGCGGGACTACCAGCTGCACATTTCCCGG
GCTGCTCTGTTTTGCAATACGCTGGTGTGTCTGCCTACCGGACTGGGAAAGACCTTTATT
GCCGCCGTGGTCATGTACAATTTCTACCGCTGGTTCCCTTCAGGAAAGGTGGTCTTCATG
GCCCCAACGAAACCCTTGGTGACACAGCAGATCGAGGCTTGCTACCAGGTGATGGGTATC
CCGCAATCCCACATGGCCGAAATGACAGGGTCTACACAAGCTTCCACCAGGAAGGAAATA
TGGTGCAGTAAGAGAGTGCTTTTTCTTACACCTCAGGTCATGGTAAATGACCTTTCTAGA
GGAGCTTGTCCCGCTGCTGAAATAAAGTGTTTAGTTATTGATGAAGCTCATAAAGCTCTC
GGAAACTATGCTTATTGCCAGGCTGTGCAACAAGTTATTACTAACCTGCTAATTGGGCAG
ATAGAGCTTCGTTCTGAAGATTCTCCAGATATTTTGACATATTCTCATGAAAGAAAAGTT
GAAAAGCTTATTGTTCCGCTTGGTGAAGAACTTGCAGCCATCCAAAAGACCTATATCCAG
ATTTTGGAATCATTTGCTCGTTCTTTGATTCAGAGGAATGTTTTGATGAGAAGGGATATC
CCAAATCTAACAAAATATCAGATAATTCTGGCAAGAGATCAGTTTAGGAAAAACCCATCT
CCGAATATTGTGGGAATACAACAAGGCATAATCGAGGGAGAGTTTGCTATTTGTATTAGT
TTATATCATGGTTATGAATTATTGCAGCAAATGGGAATGAGATCATTATATTTCTTCCTT
TGTGGAATTATGGATGGAACTAAAGGGATGACACGGTCAAAAAATGAACTTGGCCGAAAT
GAAGACTTCATGAAACTCTATAATCATCTAGAGTGTATGTTTGCACGTACACGTAGTACT
TCAGCAAATGGTATTTCTGCTATCCAACAAGGAGATAAAAATAAAAAATTTGTTTATAGT
CATCCAAAGTTAAAGAAATTAGAAGAAGTTGTAATTGAACACTTCAAGTCATGGAATGCT
GAAAACACTACTGAAAAGAAACGTGATGAGACCCGAGTTATGATCTTCTCTTCATTTCGA
GATAGTGTTCAAGAAATTGCAGAAATGCTTTCACAGCATCAGCCAATTATTAGAGTAATG
ACTTTTGTCGGCCATGCCTCAGGGAAAAGCACGAAGGGTTTTACCCAGAAGGAGCAACTG
GAGGTAGTGAAACAGTTTCGTGACGGTGGTTACAACACGCTGGTTTCTACCTGTGTGGGT
GAAGAAGGTTTGGATATAGGAGAAGTTGATCTTATAATATGTTTTGATTCCCAGAAGAGC
CCAATTCGTCTTGTACAACGAATGGGTAGAACTGGCCGTAAACGTCAAGGCAGGATAGTT
ATTATCCTTTCTGAAGGACGAGAGGAACGTATTTATAATCAGAGTCAGTCCAACAAAAGA
AGTATATATAAAGCTATTTCAAGTAACAGGCAGGTCCTTCATTTTTACCAAAGAAGTCCA
CGAATGGTTCCTGATGGAATCAACCCAAAATTACACAAAATGTTCATCACACATGGTGTC
TATGAACCAGAGAAGCCTTCTCGGAACTTGCAGCGAAAGTCATCTATCTTTTCCTATAGG
GATGGAATGAGGCAAAGTAGCCTAAAGAAAGATTGGTTCTTATCAGAAGAAGAATTTAAA
TTATGGAACAGACTTTATAGATTAAGGGACAGTGATGAAATTAAAGAGATAACATTGCCT
CAAGTTCAGTTTTCTTCTTTACAAAATGAGGAAAACAAACCAGCTCAAGAATCAACCACT
GGAATTCATCAACTCTCTCTCTCTGAATGGAGACTGTGGCAAGATCATCCTTTGCCTACA
CATCAAGTTGATCACTCAGATCGATGCCGCCATTTTATAGGCCTTATGCAAATGATAGAG
GGAATGAGACACGAAGAGGGAGAATGCAGCTATGAATTGGAAGTTGAATCTTATTTACAA
ATGGAAGATGTTACCTCAACATTTATTGCTCCCAGGAATGAATCTAATAATCTTGCCAGT
GACACCTTTATCACTCACAAGAAATCGTCATTTATAAAGAACATAAATCAAGGCAGTTCA
TCCTCAGTGATAGAATCTGATGAAGAATGTGCTGAAATTGTTAAACAAACTCATATCAAA
CCTACTAAAATTGTTTCTTTAAAGAAAAAAGTGTCTAAAGAAATAAAAAAAGATCAGCTT
AAAAAAGAAAATAATCACGGTATTATAGATTCTGTAGATAATGACAGAAATTCCACTGTT
GAAAATATTTTTCAAGAAGACCTACCAAATGATAAAAGGACATCAGATACAGATGAAATT
GCTGCCACATGTACTATTAATGAAAATGTTATTAAAGAACCGTGTGTGTTATTAACAGAG
TGTCAGTTTACAAATAAATCCACTAGTTCACTTGCTGGAAATGTTTTAGATTCTGGTTAT
AACAGTTTCAATGATGAAAAATCTGTTTCATCTAACTTATTTCTTCCATTCGAAGAAGAG
CTTTATATTGTTAGAACAGATGACCAATTTTATAATTGTCACTCATTGACAAAAGAGGTA
CTAGCTAATGTAGAGAGATTTTTATCTTATTCTCCTCCGCCTCTCAGTGGACTCTCAGAC
TTGGAATATGAAATTGCTAAGGGTACTGCACTTGAGAATTTGCTTTTCTTACCCTGTGCA
GAGCATTTACGAAGTGATAAATGCACCTGTTTGCTGTCACATTCAGCTGTGAATTCTCAA
CAGAATTTAGAATTGAATTCACTTAAATGTATAAATTATCCATCTGAAAAAAGTTGCCTT
TATGATATACCTAATGATAATATTTCTGATGAGCCAAGTCTCTGTGACTGTGATGTACAT
AAACATAATCAAAATGAAAATTTAGTACCTAACAATCGTGTTCAAATACACAGAAGCCCT
GCACAGAATTTAGTTGGAGAGAACAATCATGATGTTGATAACAGTGACCTCCCAGTATTG
TCCACTGATCAAGATGAAAGTTTGCTGTTATTTGAAGATGTTAATACAGAGTTCGACGAT
GTGAGTCTTTCACCCTTGAACAGTAAAAGCGAATCTTTACCTGTGTCAGACAAAACTGCT
ATTAGTGAAACGCCTCTGGTCTCTCAGTTCTTAATTTCTGATGAACTTTTGTTGGACAAT
AATTCTGAACTCCAAGATCAAATCACCCGTGATGCTAATAGTTTTAAATCTCGTGATCAG
AGAGGTGTACAGGAAGAAAAAGTGAAGAATCATGAGGATATTTTTGATTGCTCTAGGGAT
TTATTTTCTGTTACCTTTGATTTAGGATTCTGTAGTCCAGATTCTGATGATGAAATATTG
GAACATACATCAGATAGCAATAGACCTCTAGATGATCTATATGGAAGGTATTTGGAAATT
AAGGAGATAAGTGATGCAAATTATGTTTCGAATCAAGCACTAATACCAAGAGATCATAGT
AAAAATTTTACTAGTGGAACTGTTATTATCCCATCAAATGAAGATATGCAGAATCCAAAT
TATGTACATTTGCCACTGAGTGCAGCAAAAAATGAAGAATTGTTATCTCCTGGTTATTCT
CAGTTTTCTTTACCAGTGCAAAAAAAAGTTATGAGTACACCACTCTCTAAATCAAACACA
TTGAACTCATTTTCTAAGATAAGAAAGGAAATACTTAAGACACCAGATTCTAGTAAGGAA
AAAGTAAACCTACAAAGATTCAAAGAAGCATTGAATTCAACTTTTGATTATTCAGAATTT
TCTCTAGAAAAGTCTAAAAGCAGTGGTCCAATGTATCTGCATAAATCCTGTCATTCTGTT
GAAGATGGACAATTATTAACAAGTAACGAAAGTGAAGATGACGAGATTTTCCGAAGAAAA
GTTAAAAGAGCAAAAGGAAATGTTTTAAACTCTCCTGAGGATCAGAAAAATAGTGAAGTT
GATTCTCCACTTCATGCTGTCAAAAAGCGCAGATTTCCTATAAACAGATCAGAATTATCA
TCTAGTGATGAGAGTGAGAATTTTCCCAAACCATGTTCACAATTAGAAGACTTCAAGGTT
TGTAACGGGAATGCCAGAAGAGGCATCAAAGTCCCAAAGAGACAGAGTCACTTAAAGCAT
GTAGCTAGGAAGTTTTTAGATGATGAAGCAGAACTTTCTGAAGAAGATGCAGAATATGTT
TCATCAGATGAAAATGATGAGTCAGAAAATGAACAAGATTCCTCATTACTTGACTTTTTA
AATGATGAAACTCAACTTTCACAGGCTATAAATGATTCTGAAATGAGAGCTATTTACATG
AAATCTTTGCGTAGTCCAATGATGAACAATAAGTACAAAATGATTCATAAGACACATAAA
AACATAAACATTTTCTCGCAGATTCCTGAACAAGATGAAACCTATTTAGAGGATAGTTTT
TGTGTTGATGAAGAGGAGTCTTGCAAAGGCCAATCAAGTGAAGAAGAAGTTTGTGTTGAT
TTTAACTTAATAACTGATGATTGCTTTGCAAATAGTAAAAAGTATAAAACTCGACGTGCA
GTAATGCTAAAAGAAATGATGGAACAAAATTGTGCACATTCAAAAAAGAAATTATCCAGA
ATTATTTTACCAGATGATTCAAGTGAGGAGGAGAACAATGTAAATGATAAAAGAGAATCT
AATATTGCGGTTAACCCAAGCACTGTTAAGAAGAACAAACAACAGGACCATTGTTTAAAT
TCAGTGCCTTCTGGATCTTCTGCGCAGTCCAAGGTGCGTTCTACTCCAAGAGTTAATCCA
TTAGCAAAGCAGAGCAAACAGACATCGCTGAATTTAAAGGATACAATTTCCGAAGTCTCA
GACTTCAAACCTCAGAATCATAATGAAGTCCAGTCTACCACACCACCCTTCACTACTGTT
GATTCACAGAAAGACTGTAGAAAATTTCCAGTTCCACAGAAGGATGGTAGTGCTTTGGAG
GATTCTAGCACTTCAGGGGCATCCTGTTCCAAGTCAAGACCACATTTAGCTGGGACACAT
ACTTCTCTTAGACTTCCGCAGGAAGGAAAAGGAACCTGTATTCTTGTAGGTGGTCATGAA
ATCACTTCTGGATTAGAAGTAATTTCTTCCCTAAGAGCAATTCATGGGTTGCAAGTAGAA
GTTTGTCCTCTTAATGGCTGTGATTACATCGTGAGTAATCGCATGGTGGTGGAAAGGAGG
TCTCAATCTGAGATGTTAAATAGTGTCAATAAGAACAAGTTCATTGAGCAGATCCAGCAC
CTGCAGAGTATGTTTGAAAGAATATGTGTGATTGTGGAAAAGGACAGAGAAAAAACAGGA
GACACATCAAGGATGTTTAGGAGAACAAAGAGCTATGACAGCCTGCTGACTACCTTAATT
GGCGCTGGAATCCGAATTCTTTTCAGTTCCTGCCAAGAAGAAACCGCAGATTTGCTAAAG
GAACTGTCTTTAGTGGAACAAAGAAAGAATGTTGGTATTCATGTTCCAACAGTGGTGAAT
AGTAATAAAAGTGAGGCACTCCAGTTTTATTTAAGTATTCCCAATATAAGTTATATAACT
GCATTAAATATGTGTCACCAGTTTTCATCTGTGAAAAGGATGGCTAACAGCTCACTTCAA
GAAATCTCCATGTATGCACAAGTAACTCATCAGAAGGCTGAGGAGATCTATAGATATATT
CACTATGTATTTGACATACAAATGTTACCAAATGATCTTAACCAAGATAGACTGAAATCT
GATATATAA
Protein sequence predicted based on nucleotide sequence
MSGRQRTLFQTWGSSISRSSGTPGCSSGTERPQSPGSSKAPLPAAAEAQLESDDDVLLVA AYEAERQLCLENGGFCTSAGALWIYPTNCPVRDYQLHISRAALFCNTLVCLPTGLGKTFI AAVVMYNFYRWFPSGKVVFMAPTKPLVTQQIEACYQVMGIPQSHMAEMTGSTQASTRKEI WCSKRVLFLTPQVMVNDLSRGACPAAEIKCLVIDEAHKALGNYAYCQAVQQVITNLLIGQ IELRSEDSPDILTYSHERKVEKLIVPLGEELAAIQKTYIQILESFARSLIQRNVLMRRDI PNLTKYQIILARDQFRKNPSPNIVGIQQGIIEGEFAICISLYHGYELLQQMGMRSLYFFL CGIMDGTKGMTRSKNELGRNEDFMKLYNHLECMFARTRSTSANGISAIQQGDKNKKFVYS HPKLKKLEEVVIEHFKSWNAENTTEKKRDETRVMIFSSFRDSVQEIAEMLSQHQPIIRVM TFVGHASGKSTKGFTQKEQLEVVKQFRDGGYNTLVSTCVGEEGLDIGEVDLIICFDSQKS PIRLVQRMGRTGRKRQGRIVIILSEGREERIYNQSQSNKRSIYKAISSNRQVLHFYQRSP RMVPDGINPKLHKMFITHGVYEPEKPSRNLQRKSSIFSYRDGMRQSSLKKDWFLSEEEFK LWNRLYRLRDSDEIKEITLPQVQFSSLQNEENKPAQESTTGIHQLSLSEWRLWQDHPLPT HQVDHSDRCRHFIGLMQMIEGMRHEEGECSYELEVESYLQMEDVTSTFIAPRNESNNLAS DTFITHKKSSFIKNINQGSSSSVIESDEECAEIVKQTHIKPTKIVSLKKKVSKEIKKDQL KKENNHGIIDSVDNDRNSTVENIFQEDLPNDKRTSDTDEIAATCTINENVIKEPCVLLTE CQFTNKSTSSLAGNVLDSGYNSFNDEKSVSSNLFLPFEEELYIVRTDDQFYNCHSLTKEV LANVERFLSYSPPPLSGLSDLEYEIAKGTALENLLFLPCAEHLRSDKCTCLLSHSAVNSQ QNLELNSLKCINYPSEKSCLYDIPNDNISDEPSLCDCDVHKHNQNENLVPNNRVQIHRSP AQNLVGENNHDVDNSDLPVLSTDQDESLLLFEDVNTEFDDVSLSPLNSKSESLPVSDKTA ISETPLVSQFLISDELLLDNNSELQDQITRDANSFKSRDQRGVQEEKVKNHEDIFDCSRD LFSVTFDLGFCSPDSDDEILEHTSDSNRPLDDLYGRYLEIKEISDANYVSNQALIPRDHS KNFTSGTVIIPSNEDMQNPNYVHLPLSAAKNEELLSPGYSQFSLPVQKKVMSTPLSKSNT LNSFSKIRKEILKTPDSSKEKVNLQRFKEALNSTFDYSEFSLEKSKSSGPMYLHKSCHSV EDGQLLTSNESEDDEIFRRKVKRAKGNVLNSPEDQKNSEVDSPLHAVKKRRFPINRSELS SSDESENFPKPCSQLEDFKVCNGNARRGIKVPKRQSHLKHVARKFLDDEAELSEEDAEYV SSDENDESENEQDSSLLDFLNDETQLSQAINDSEMRAIYMKSLRSPMMNNKYKMIHKTHK NINIFSQIPEQDETYLEDSFCVDEEESCKGQSSEEEVCVDFNLITDDCFANSKKYKTRRA VMLKEMMEQNCAHSKKKLSRIILPDDSSEEENNVNDKRESNIAVNPSTVKKNKQQDHCLN SVPSGSSAQSKVRSTPRVNPLAKQSKQTSLNLKDTISEVSDFKPQNHNEVQSTTPPFTTV DSQKDCRKFPVPQKDGSALEDSSTSGASCSKSRPHLAGTHTSLRLPQEGKGTCILVGGHE ITSGLEVISSLRAIHGLQVEVCPLNGCDYIVSNRMVVERRSQSEMLNSVNKNKFIEQIQH LQSMFERICVIVEKDREKTGDTSRMFRRTKSYDSLLTTLIGAGIRILFSSCQEETADLLK ELSLVEQRKNVGIHVPTVVNSNKSEALQFYLSIPNISYITALNMCHQFSSVKRMANSSLQ EISMYAQVTHQKAEEIYRYIHYVFDIQMLPNDLNQDRLKSDI*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pcDNA3.1-3xFLAG-V5-ccdB
Synonyms:
'
Sequencing Primer:
Forward:
CMV forward
Reverse:
Unknown
Description:
with tag/fusion/marker assay, Gateway®, donor (entry) clone, mammalian expression expression with CMV enhancer and CMV Promoter and 3xFlag and V5 and attR1 and lac UV5 promoter and CmR and ccdB and attR2 and BGH pA and f1 ori and SV40 promoter and SV40 Ori and HygR and SV40 poly(A) signal and M13 reverse and lac operator and lac promoter and CAP binding site and ori and AmpR and AmpR promoter; ampicillin resistance in bacterial;
Comments:
None
Size (bp):
7361
Parent Vector:
None
Properties:
Gateway, donor (entry), mammalian expression, with tag/fusion/marker
Author Name:
Economos NG
Thapar U
Balasubramanian N
Karras GI
Glazer PM
Thapar U
Balasubramanian N
Karras GI
Glazer PM
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
3xFlag
three tandem FLAGŪ epitope tags and an enterokinase cleavage site
908
973
CDS
V5
epitope tag from simian virus 5
992
1033
CDS
CmR
confers resistance to chloramphenicol
1264
1923
CDS
ccdB
bacterial toxin that poisons DNA gyrase
2265
2570
CDS
HygR
confers resistance to hygromycin
3882
4907
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
6365
7225
enhancer
CMV enhancer
human cytomegalovirus immediate early enhancer
235
614
poly-A signal
BGH pA
bovine growth hormone polyadenylation signal
2792
3016
poly-A signal
SV40 poly(A) signal
SV40 polyadenylation signal
5037
5158
primer_bind
M13 reverse
common sequencing primer
5207
5223
promoter
CMV Promoter
human cytomegalovirus immediate early promoter
615
818
promoter
lac UV5 promoter
E. coli lac promoter with an "up" mutation
1180
1210
promoter
SV40 promoter
SV40 enhancer and early promoter
3504
3833
promoter
lac promoter
promoter for the E. coli lac operon
5255
5285
promoter
AmpR promoter
null
7226
7330
protein binding site
attR1
recombination site for the Gateway LR reaction
1031
1155
protein binding site
attR2
recombination site for the Gateway LR reaction
2611
2735
protein binding site
lac operator
binding site for lac repressor to inhibit transcription in E. coli
5231
5247
protein binding site
CAP binding site
CAP binding activates transcription in the presence of cAMP.
5300
5321
rep_origin
f1 ori
f1 bacteriophage origin of replication
3062
3490
rep_origin
SV40 Ori
SV40 origin of replication
3684
3819
rep_origin
ori
origin of replication
5609
6194
