Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pcDNA3.1-ccdB-V5-3xFLAG
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Economos NG
Thapar U
Balasubramanian N
Karras GI
Glazer PM
Thapar U
Balasubramanian N
Karras GI
Glazer PM
HsCD00970657
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2589nts
Open reading frame: 1 to 2589
ATGTCGTCCCCGGCGTCGACCCCGAGCCGCCGCGGCAGCCGGCGTGGAAGGGCCACCCCC
GCCCAGACGCCTCGGAGTGAGGATGCCAGGTCATCTCCCTCTCAGAGACGTAGAGGCGAG
GATTCCACCTCCACGGGGGAGTTGCAGCCGATGCCAACCTCGCCTGGAGTGGACCTGCAG
AGCCCTGCTGCGCAGGACGTGCTGTTTTCCAGCCCTCCCCAAATGCATTCTTCAGCTATC
CCTCTTGACTTTGATGTTAGTTCACCACTGACATACGGCACTCCCAGCTCTCGGGTAGAG
GGAACCCCAAGAAGTGGTGTTAGGGGCACACCTGTGAGACAGAGGCCTGACCTGGGCTCT
GCACAGAAGGGCCTGCAAGTGGATCTGCAGTCTGACGGGGCAGCAGCAGAAGATATAGTG
GCAAGTGAGCAGTCTCTAGGCCAAAAACTTGTGATCTGGGGAACAGATGTAAATGTGGCA
GCATGCAAAGAAAACTTTCAGAGATTTCTTCAGCGTTTTATTGACCCTCTGGCTAAAGAA
GAAGAAAATGTTGGCATAGATATTACTGAACCTCTATACATGCAACGACTTGGGGAGATT
AATGTTATTGGTGAGCCATTTTTAAATGTGAACTGTGAACACATCAAATCATTTGACAAA
AATTTGTACAGACAACTCATCTCTTACCCACAGGAAGTTATTCCAACTTTTGACATGGCT
GTCAATGAAATCTTCTTTGACCGTTACCCTGACTCAATCTTAGAACATCAGATTCAAGTA
AGACCATTCAACGCATTGAAGACTAAGAATATGAGAAACCTGAATCCAGAAGACATTGAC
CAGCTCATCACCATCAGCGGCATGGTGATCAGGACATCCCAGCTGATTCCCGAGATGCAG
GAGGCCTTCTTCCAGTGCCAAGTGTGTGCCCACACGACCCGGGTGGAGATGGACCGCGGC
CGCATTGCAGAGCCCAGTGTGTGCGGGCGCTGCCACACCACCCACAGCATGGCACTCATC
CACAACCGCTCCCTCTTCTCTGACAAGCAGATGATCAAGCTTCAGGAGTCTCCGGAAGAC
ATGCCTGCAGGGCAGACACCACACACAGTTATCCTGTTTGCTCACAATGATCTCGTTGAC
AAGGTCCAGCCTGGGGACAGAGTGAATGTTACAGGCATCTATCGAGCTGTGCCTATTCGA
GTCAATCCAAGAGTGAGTAATGTGAAGTCTGTCTACAAAACCCACATTGATGTCATTCAT
TATCGGAAAACGGATGCAAAACGTCTGCATGGCCTTGATGAAGAAGCAGAACAGAAACTT
TTTTCAGAGAAACGTGTGGAATTGCTTAAGGAACTTTCCAGGAAACCAGACATTTATGAG
AGGCTTGCTTCAGCCTTGGCTCCAAGCATTTATGAACATGAAGATATAAAGAAGGGAATT
TTGCTTCAGCTCTTTGGCGGGACAAGGAAGGATTTTAGTCACACTGGAAGGGGCAAATTT
CGGGCTGAGATCAACATCTTGCTGTGTGGCGACCCTGGTACCAGCAAGTCCCAGCTGCTG
CAGTACGTGTACAACCTCGTCCCCAGGGGCCAGTACACGTCTGGGAAGGGCTCCAGTGCA
GTTGGCCTCACTGCGTACGTAATGAAAGACCCTGAGACAAGGCAGCTGGTCCTGCAGACA
GGTGCTCTTGTCCTGAGTGACAACGGCATCTGCTGTATCGATGAGTTCGACAAGATGAAT
GAAAGTACAAGATCGGTATTGCATGAAGTCATGGAACAGCAGACTCTGTCCATTGCAAAG
GCTGGGATCATCTGTCAGCTCAATGCGCGCACCTCTGTCCTGGCAGCAGCAAATCCCATT
GAGTCTCAGTGGAATCCTAAAAAAACAACCATTGAAAACATCCAGCTGCCTCATACTTTA
TTATCAAGGTTTGATTTGATCTTCCTCATGCTGGACCCTCAGGACGAAGCCTATGACAGG
CGTCTGGCTCACCACCTGGTCGCACTGTACTACCAGAGCGAGGAGCAGGCAGAGGAGGAG
CTCCTGGACATGGCGGTGCTAAAGGACTACATTGCCTACGCGCACAGCACCATCATGCCG
CGGCTAAGTGAGGAAGCCAGCCAGGCTCTCATCGAGGCTTATGTAGACATGAGGAAGATT
GGCAGTAGCCGGGGAATGGTTTCTGCATACCCTCGACAGCTAGAGTCATTAATCCGCTTA
GCAGAAGCCCATGCTAAAGTAAGATTGTCTAACAAAGTTGAAGCCATTGATGTGGAAGAG
GCCAAACGCCTCCATCGGGAAGCTCTGAAGCAGTCTGCAACTGATCCCCGGACTGGCATC
GTGGACATATCTATTCTTACTACGGGGATGAGTGCCACCTCTCGTAAACGGAAAGAAGAA
TTAGCTGAAGCATTGAAAAAGCTTATTTTATCTAAGGGCAAAACACCAGCTCTAAAATAC
CAGCAACTTTTTGAAGATATTCGGGGACAATCTGACATAGCAATTACTAAAGATATGTTT
GAAGAAGCACTGCGTGCCCTGGCAGATGATGATTTCCTGACAGTGACTGGGAAGACCGTG
CGCTTGCTC
Protein sequence predicted based on nucleotide sequence
MSSPASTPSRRGSRRGRATPAQTPRSEDARSSPSQRRRGEDSTSTGELQPMPTSPGVDLQ SPAAQDVLFSSPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPDLGS AQKGLQVDLQSDGAAAEDIVASEQSLGQKLVIWGTDVNVAACKENFQRFLQRFIDPLAKE EENVGIDITEPLYMQRLGEINVIGEPFLNVNCEHIKSFDKNLYRQLISYPQEVIPTFDMA VNEIFFDRYPDSILEHQIQVRPFNALKTKNMRNLNPEDIDQLITISGMVIRTSQLIPEMQ EAFFQCQVCAHTTRVEMDRGRIAEPSVCGRCHTTHSMALIHNRSLFSDKQMIKLQESPED MPAGQTPHTVILFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHIDVIH YRKTDAKRLHGLDEEAEQKLFSEKRVELLKELSRKPDIYERLASALAPSIYEHEDIKKGI LLQLFGGTRKDFSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSA VGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAK AGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDEAYDR RLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKI GSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGI VDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMF EEALRALADDDFLTVTGKTVRLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pcDNA3.1-ccdB-V5-3xFLAG
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
V5 reverse
Description:
with tag/fusion/marker assay, Gateway®, donor (entry) clone, mammalian expression expression with CMV enhancer and CMV Promoter and attR1 and lac UV5 promoter and CmR and ccdB and attR2 and V5 and 3xFlag and BGH pA and f1 ori and SV40 promoter and SV40 Ori and HygR and SV40 poly(A) signal and M13 reverse and lac operator and lac promoter and CAP binding site and ori and AmpR and AmpR promoter and T7 promoter; ampicillin resistance in bacterial;
Comments:
None
Size (bp):
7419
Parent Vector:
None
Properties:
Gateway, donor (entry), mammalian expression, with tag/fusion/marker
Author Name:
Economos NG
Thapar U
Balasubramanian N
Karras GI
Glazer PM
Thapar U
Balasubramanian N
Karras GI
Glazer PM
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
CmR
provides resistance to chloramphenicol
1264
1923
CDS
ccdB
bacterial toxin that poisons DNA gyrase
2265
2570
CDS
V5
epitope tag from simian virus 5
992
1033
CDS
3xFlag
three tandem FLAG epitope tags and an enterokinase cleavage site
908
973
CDS
HygR
provides resistance to hygromycin
3882
4907
CDS
AmpR
provides resistance to ampicillin, carbenicillin, and related antibiotics
6365
7225
enhancer
CMV enhancer
human cytomegalovirus immediate early enhancer
235
614
poly-A signal
BGH pA
polyadenylation signal for bovine growth hormone
2792
3016
poly-A signal
SV40 poly(A) signal
SV40 polyadenylation signal
5037
5158
primer_bind
M13 reverse
common sequencing primer
5207
5223
promoter
CMV Promoter
human cytomegalovirus immediate early promoter
615
818
promoter
lac UV5 promoter
E. coli lac promoter with a mutation to trigger transcription initiation
1180
1210
promoter
SV40 promoter
SV40 enhancer and early promoter
3504
3833
promoter
lac promoter
promoter for the E. coli lac operon
5255
5285
promoter
AmpR promoter
null
7226
7330
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
863
881
protein binding site
attR1
recombination site for the Gateway LR reaction
1031
1155
protein binding site
attR2
recombination site for the Gateway LR reaction
2611
2735
protein binding site
lac operator
binding site for lac repressor to inhibit transcription in E. coli.
5255
5285
protein binding site
CAP binding site
CAP binding site to activate transcription in the presence of cAMP
5300
5321
rep_origin
f1 ori
f1 bacteriophage origin of replication
3062
3490
rep_origin
SV40 Ori
SV40 origin of replication
3684
3819
rep_origin
ori
origin of replication
5609
6194
