Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH464148.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
ZM552
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
ScCD00098635
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4740nts
Open reading frame: 1 to 4740
ATGTCGCATTCAGACTACTTCAATTATAAGCCTTACGGCGATTCCACGGAAAAGCCCAGC
AGCTCCAAGATGAGGCAGTCATCTTCATCATCTTCATCTAGACTAAGGTCGGAAAGTTTA
GGGCGAAATTCCAATACTACGCAGGCTCGAGTAGCGTCATCGCCCATCAGCCCAGGGCTG
CATTCTACCCAGTACTTTAGATCACCAAACGCTGTTTACAGCCCTGGAGAGTCTCCATTA
AATACAGTACAGCTATTCAATCGTCTTCCGGGTATACCTCAAGGTCAGTTTTTTCATCAA
AATGCCATTTCTGGGTCCTCCAGCTCCTCGGCAAGATCTAGTAGACGGCCCTCGAATATT
GGTCTTCCTCTACCAAAAAATCCTCAGCAGTCTCTACCGAAGTTGTCTACTCAACCTGTT
CCTGTACATAAGAAAGTTGAGGCTAGTAAAACAGAGAGCGAGATTATTAAGAAGCCCGCT
CCCGTGAATAGTAATCAAGATCCATTATTGACGACCCCAACGTTAGTGATATCACCAGAA
CTGGCTTCACTAAATACAACGAATACATCGATTATGTCAACGCCACAAAATATTACAAAC
CAAACTTCGAACAAACACATTCCCACCAGATCGCAACCAAATGGGTCAACAAGTTCCTCC
ACTTTGCAGGATATTGTCACGACAAATAGCTCGCAACGGTCTGTAGGCCACCATGGTGGA
AGCACAACGAGCCTCCGAACATACAAAAAACAATATGTATTAAATGAACAGTTATATTTA
AGAAAAATGAGAAACCGTGCTAATGATGATTATTACACTAGAGGTATAGTCGCATCATCC
AACTTTGAAGATGACGAAGAAAATTTTAGTAACAAAGGTGAAGATGACTTAGAACTAGAA
ATGGATGATCTTTTAAAGGTAGAAGGTGAGGATAAAGATAACGACTTCAATTTTGGTTAT
AATTTTATTACGTCGAGCACAAAAAATAATGAAAATGTTGTTTCGATGAGCCTAAATTAT
CTAAAGGGCAAATTGGATTGGTTGAGGGATGTGAACAATGATCAACCGTGTGAAATAGAA
GATGAGGAGTGGCATTCCATACTGGGGAGCGAGGATTTGCTGTCAAAATTGTTACAAAAT
CCTATGGTGAACAACCGATTTGAATGGCAAACAATGTTATCTAAGGTACTGAAGGGAGAT
ATTGTGAGGAATGAAAAAACGAAGATTGCAAATCAAGGGAAAGGCCCTGGCTTCAATACT
CAGTTTTCAGATGACATTTGGATTGAGTTGAAGGCATGGATGAATGGGAGGACCGTGGAA
GATCAGAACAAATCTCTGAGGATTTTTAGGGATTCTACTGACTCCGTATTTCAAGAAATC
ATGGCCTTTAAACTAGAAGATAATATGAGCGCTGACGAGGCTGCAGAGACTATCAAATCA
CTAGTAGACAAATATTATAGAGTCTTAAATCTATGGCCTAACATTAAAAGAATGCATGCT
GAAAAACCCATTACTAAAACAGAAGCATTCAGGAATCGAATAGATACTTTGAATAGTTGG
CTTAATTTCAAATTTAACTTTGATACTAATATTGCGTACCTGAAAAAATGGATAGTTGGC
AATAAAGAGCTAGAAAGCACTACCGAAGTGGATAACACCACCGTGAATTTGGATGATCCA
GCCGTTTTCGCCACTAATTGTAAACGCTTTGCGGAGCAAATTATGAAGGAAAAGGATATT
GAACTGATATTTCAAAAAAAAATATTCTTTCCATTAGCACCATGGATTTTGAAGGCCAAA
TTTTTCTTCTTGAAATACCAAAAAACTTGGAATGAATTGAATCTATCTTATTTGGATCAA
GATCTGGAATTTTTATTGATGTTTCCAATGCGTTTGGTAAAAGATATAATACTAATTCGC
CTATCTTACGCGAAGAAAATACAGAATCCAACCTTGATGATGATCGATCAAATGATGGAC
GATTTTAGTACATATATTAAGTTGGCAGTCCAAATGAAATTCACTGTTGCTTCTTACTGT
AATGACTGGTTTTTTAAAGTGAAAATCGATCCCGAATTTGATCATACCGTTGTTGAAGGA
TTGGAATATTTTTTCTCCATTTTGGAACTGAGAATATTATATAGTGGCAAAAACTCATTC
AAGACTTCTAAAGAACCTGATCTGTTATTAAAATATTGGGAAATGTTTAGAAACGTCGGC
TATTATATTGATGATGCAGGCGAACTGATCGCAGCAGAATTTACGAAGTTGACACTTAGA
TTGGTTCACAGATTGCACGCTTACCTTTTAAGGCAGCAAAACACTCCGCCAAAATTAGAG
AATGAAGCTGCTGCTGAAAAATGGCTGGTGCAAATATTCGAAATACTTGGATCCATGAAA
AGAAAACTCAATAGATTCACCAATATTTTGACAAAAGCATTTCAAAATTTTGTCCGCTAC
AAGATAGAAGATCACAACTATCTGCTAAAGCAACTAAAAGAAACAGGCCACTTTCTTATA
TACACAGGAGGTTACCTAGAGCAAAATGGTACTTATTTAATTGGTAGCCCAGAGCTATTA
GGCTGTAAAGATGATGATATTTTAAGAATCATTAAGAATTCAGACATCGGTTGTGATTTA
GTGCCTAAGCTAGAAATTAATAACAGTCTGACAATTTATAATGCTTTGGATGATAATTGG
AACTCCAACTCATCACTGGGTTCAGATATCTCGAATGATGGTACCCCATTTTATTATATC
AAAAACGATTTGACCACCCAGCCTAGATCTTATAACGGTAATAGAGTCAATCGTGAACCG
GATTTTGAAAACAGCAGGAGCACGGAGGAAGAGTTTTATGAACTGGAGACAAGATTGAAC
TCTCTTGGTTATGTATTGGTATTGACTCCGCAAGAGCCATTACTTTGGGAGGGTGAGATG
TATAATCTATCCGATAACAAAACAATTAAACCAGAGGGATTGAACTTGAAAGTAATTCCT
AATTCAATAGATTTGATGTGCCAAGGATCCAGTTATGCCTTAGAATACCAATGTGACAGG
TTCCAACAGATATCTGGTAGCTCAGTTTCTTTCTTGGAAAAAAAATCTTCCTCAGAAACG
GTTAAAAACAACTTACAAAGGATAAATAAAGCATATTTCAGATGCACGTACAGTGTTCTG
AAGAACTATACAAAGATTGTGACCACGTTCAAGAAGGTAAGTCCTGTCAATGATTTATTG
AATAATATTTTCCTTTTTGGGAGGGATTTTGGTCTGAACTTTTTGAGAATTAATGTTGCC
AACAATGAAAAAAGATCCATTATAATACTTTTAATGATGCGGTTAAGTATCGGATGGCTG
AAGTTCTTAGCTGAAGACTGTGATCCGACTGATCAAAGGGTATTTAGGTGGTGTGTCACG
TCAATGGAGTTCGCGATGCATATGGTAAGCGGTTGGAACATACTAGCTCTTGATGAATGC
CAATTTTCTTCTTTAAAACAAAAAATTTCAGAATGCATGTCATTACTTATTTCTCATTTT
GATATAATAGGCGCACGCTCCATAGAAGTTGAGAAAATCAATCAACAAGCTAGATCAAAT
CTTGATTTGGAAGATGTGTTTGACGATGATATGATGCTACAAGTGAATTCCGAGTTCCGA
GTACAAAGTATAATGGAATTGGAAGAAAGGATAAAGCGGAATCCTCATCAAACTGGTAAA
GTAATTGATGATAGTGACAAAGGTAACAAGTACCTTGTTTCTTTGGCATCTTCCATATCG
AACGTTTCTATGAGATGGCAAAAGAGGAACTTTATTGGTGGCGGTACTTTTGGAAGGGTA
TATTCTGCTGTTGATTTGGATAATGGTGAGATTTTAGCAGTCAAGGAAATCAATATTCAA
GATAGCAAATCAATGCAAAAAATATTCCCCTTAATCAAGGAGGAAATGAGTGTCTTAGAG
ATATTGAACCATCCAAATATAGTTTCATATTACGGTGTTGAAGTTCATCGTGATAAAGTT
AACATCTTTATGGAATATTGTGAAGGCGGTTCCCTAGCAGCTCTTTTGGAGCATGGTCGT
ATTGAAGATGAAATGGTCACTCAAGTCTACACTTTACAATTGCTAGAAGGACTTGCATAT
TTGCATGAATCCGGCATTGTTCACCGTGATGTTAAACCCGAAAACATCCTACTAGATTTT
AATGGTGTTATTAAGTATGTTGATTTTGGTGCTGCTAAAAAAATTGCTAATAATGGAACT
AGATTGGCAAGTATGAACAAAATCGAAAACGCAGATGGTGAACACGAAGATGTTACCCAT
GTTTCTGATTCAAAGGCAGTGAAAAATAACGAAAATGCTCTATTAGACATGATGGGAACT
CCTATGTACATGGCTCCAGAATCCATCACTGGATCTACCACCAAAGGCAAACTTGGGGCA
GACGATGTTTGGTCGTTAGGCTGCGTGGTATTAGAAATGATCACTGGTAGACGGCCATGG
GCTAACTTAGATAATGAATGGGCTATCATGTACCATGTTGCTGCAGGACATACCCCACAA
TTCCCTACTAAGGATGAAGTGTCGTCTGCTGGTATGAAATTTCTGGAAAGATGTCTTATT
CAAAACCCCTCCAAGAGAGCCAGTGCGGTTGAGCTGTTGATGGATCCTTGGATTGTACAA
ATTAGAGAAATAGCCTTTGGCGACGATTCTTCCTCTACAGATACTGAGGAAAGAGAGTAG
Protein sequence predicted based on nucleotide sequence
MSHSDYFNYKPYGDSTEKPSSSKMRQSSSSSSSRLRSESLGRNSNTTQARVASSPISPGL HSTQYFRSPNAVYSPGESPLNTVQLFNRLPGIPQGQFFHQNAISGSSSSSARSSRRPSNI GLPLPKNPQQSLPKLSTQPVPVHKKVEASKTESEIIKKPAPVNSNQDPLLTTPTLVISPE LASLNTTNTSIMSTPQNITNQTSNKHIPTRSQPNGSTSSSTLQDIVTTNSSQRSVGHHGG STTSLRTYKKQYVLNEQLYLRKMRNRANDDYYTRGIVASSNFEDDEENFSNKGEDDLELE MDDLLKVEGEDKDNDFNFGYNFITSSTKNNENVVSMSLNYLKGKLDWLRDVNNDQPCEIE DEEWHSILGSEDLLSKLLQNPMVNNRFEWQTMLSKVLKGDIVRNEKTKIANQGKGPGFNT QFSDDIWIELKAWMNGRTVEDQNKSLRIFRDSTDSVFQEIMAFKLEDNMSADEAAETIKS LVDKYYRVLNLWPNIKRMHAEKPITKTEAFRNRIDTLNSWLNFKFNFDTNIAYLKKWIVG NKELESTTEVDNTTVNLDDPAVFATNCKRFAEQIMKEKDIELIFQKKIFFPLAPWILKAK FFFLKYQKTWNELNLSYLDQDLEFLLMFPMRLVKDIILIRLSYAKKIQNPTLMMIDQMMD DFSTYIKLAVQMKFTVASYCNDWFFKVKIDPEFDHTVVEGLEYFFSILELRILYSGKNSF KTSKEPDLLLKYWEMFRNVGYYIDDAGELIAAEFTKLTLRLVHRLHAYLLRQQNTPPKLE NEAAAEKWLVQIFEILGSMKRKLNRFTNILTKAFQNFVRYKIEDHNYLLKQLKETGHFLI YTGGYLEQNGTYLIGSPELLGCKDDDILRIIKNSDIGCDLVPKLEINNSLTIYNALDDNW NSNSSLGSDISNDGTPFYYIKNDLTTQPRSYNGNRVNREPDFENSRSTEEEFYELETRLN SLGYVLVLTPQEPLLWEGEMYNLSDNKTIKPEGLNLKVIPNSIDLMCQGSSYALEYQCDR FQQISGSSVSFLEKKSSSETVKNNLQRINKAYFRCTYSVLKNYTKIVTTFKKVSPVNDLL NNIFLFGRDFGLNFLRINVANNEKRSIIILLMMRLSIGWLKFLAEDCDPTDQRVFRWCVT SMEFAMHMVSGWNILALDECQFSSLKQKISECMSLLISHFDIIGARSIEVEKINQQARSN LDLEDVFDDDMMLQVNSEFRVQSIMELEERIKRNPHQTGKVIDDSDKGNKYLVSLASSIS NVSMRWQKRNFIGGGTFGRVYSAVDLDNGEILAVKEINIQDSKSMQKIFPLIKEEMSVLE ILNHPNIVSYYGVEVHRDKVNIFMEYCEGGSLAALLEHGRIEDEMVTQVYTLQLLEGLAY LHESGIVHRDVKPENILLDFNGVIKYVDFGAAKKIANNGTRLASMNKIENADGEHEDVTH VSDSKAVKNNENALLDMMGTPMYMAPESITGSTTKGKLGADDVWSLGCVVLEMITGRRPW ANLDNEWAIMYHVAAGHTPQFPTKDEVSSAGMKFLERCLIQNPSKRASAVELLMDPWIVQ IREIAFGDDSSSTDTEERE*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
4740
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
LEU2
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
ZM552
Synonyms:
ZM552
Description:
Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
Comments:
ZM511 Stu1 vector plus Gateway attR frame B cassette EcoRV fragment; same as ZM515. Cloning into the vector removes the ccdB cassette and chloramphenicol resistance.
Size (bp):
8302
Parent Vector:
None
Properties:
Gateway, acceptor (destination), recombinational cloning, with tag/fusion/marker, yeast expression
Author Name:
Zarmik Moqtaderi
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
primer site
M13F
M13 forward sequencing primer
8286
8301
primer site
M13R
M13 reverse sequencing primer
2630
2647
promoter
CMD1
CMD1 promoter
295
484
recombination site
attR1
attR1 recombinational cloning site
650
666
recombination site
attR2
attR2 recombination site
2337
2352
reporter gene
LacZ
LacZ alpha gene
8146
8214
selectable marker
AmpR
ampcillin resistance
3795
4454
selectable marker
CmR
chloramphenicol resistance. removed with the ccdB cassette in clones with insert
883
1539
selectable marker
LEU2
LEU2 selectable marker in yeast
5273
5344
tag
V5
N-terminal triple V5 tag
494
631
trxn termination sequence
CYC1
CYC1 terminator
2377
2582
yeast ARS
ARS
ARS yeast origin of replication
0
0
yeast centromere
CEN
yeast centromere
0
0
