Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
FLH464375.01X
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
ZM552
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
ScCD00099154
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3108nts
Open reading frame: 1 to 3108
ATGGAAGAGCTGAATATTGATTTTGACGTATTTAAGAAAAGGATTGAATTGTTGTATTCC
AAGTACAATGAGTTTGAAGGTTCTCCAAATTCGCTGCTGTTTGTTTTAGGTTCGTCCAAC
GCTGAAAACCCGTACCAGAAGACGACCATATTGCATAATTGGTTGCTAAGTTACGAATTT
CCCGCTACTTTGATTGCATTAGTTCCCGGAAAGGTTATTATAATTACTAGTTCTGCCAAG
GCCAAGCATTTACAAAAGGCAATTGATCTATTTAAAGACCCTGAAAGCAAAATTACGCTG
GAATTGTGGCAAAGAAACAATAAAGAACCAGAACTCAATAAAAAGTTATTTGATGACGTT
ATTGCCTTAATCAATAGCGCTGGTAAAACAGTGGGTATCCCTGAAAAGGACTCTTACCAA
GGTAAATTTATGACTGAGTGGAACCCAGTATGGGAAGCGGCTGTGAAGGAGAACGAATTC
AATGTCATTGATATTTCGCTGGGTCTTTCTAAAGTTTGGGAAGTGAAGGATGTTAACGAA
CAAGCCTTCCTGTCCGTTTCAAGTAAGGGGTCGGACAAATTTATGGACCTTTTATCCAAT
GAAATGGTTCGGGCAGTTGACGAGGAGTTGAAAATCACCAATGCCAAGTTATCAGACAAA
ATTGAAAATAAAATTGATGATGTGAAATTTTTGAAGCAATTGAGTCCCGATTTAAGCGCA
TTATGCCCACCAAACTACAAATTCAACTTTGATTTATTGGATTGGACCTATTCTCCAATC
ATTCAGTCCGGGAAAAAGTTTGATCTTAGGGTTTCTGCCCGTTCTACCAATGATCAGTTG
TACGGCAACGGTTGTATTTTAGCTTCATGTGGTATTCGTTACAATAATTATTGTTCTAAT
ATTACTAGGACCTTTTTGATCGATCCATCTGAAGAAATGGCCAACAACTACGATTTTCTA
TTAACTTTGCAAAAAGAAATTGTAACTAACATTCTGAAGCCCGGCCGTACACCAAAAGAA
GTTTATGAATCAGTAATAGAATACATCGAAAAAACCAAACCTGAACTAGTGCCCAATTTC
ACCAAAAACATTGGTTCATTGATTGGTCTCGAATTTAGAGATTCTAACTTTATATTAAAT
GTTAAGAATGATTACCGTAAAATCCAACGCGGTGACTGCTTTAATATTTCGTTTGGTTTC
AATAATCTGAAAGATTCTCAAAGTGCTAACAACTACGCTTTACAATTAGCTGATACGGTT
CAAATTCCTCTCGATGAAACAGAGCCTCCACGCTTCTTAACAAATTACACCAAAGCCAAA
TCCCAGATATCATTTTATTTCAATAATGAAGAAGAAGACAATAATAAAAAGAAATCTTCA
CCTGCCACCAAAGTCCCTAGTAAACCAGACAGAAATTCCAAAATTTTAAGAACAAAGTTA
CGTGGCGAAGCCCGTGGTGGTGCCGAAGACGCTCAAAAGGAGCAAATTCGTAAAGAAAAC
CAAAAGAAGCTACACGAAAAATTGGAAAAGAATGGATTACTCAGATTTAGTGCTGCTGAT
GCCAATGGCCCAGATAGTGAACCTCGTCAATACTTCAAAAAATATGAATCTTATGTTCGG
GATTCACAACTACCAACCAATATTCGTGATCTAAGAATTCATGTCGACTGGAAAAGCCAA
ACAATTATTCTACCCATTTACGGTAGGCCAGTCCCATTTCATATAAATTCATATAAGAAT
GGTTCTAAAAACGAAGAAGGCGAATATACGTATTTACGTTTGAACTTTAATTCACCGGGA
TCTTCTGGTGGTATTTCTAAAAAAGTGGAGGAATTGCCGTATGAGGAATCAGCAGACAAT
CAATTTATACGTTCGATTACACTAAGATCCAAAGATGGTGACCGCATGAGTGAAACCTTT
AAACAAATTGCAGATTTGAAAAAAGAAGCCACAAAGAGAGAGCAAGAACGTAAGGCGCTT
GCTGATGTTGTTCAACAGGACAAATTGATTGAAAATAAAACTGGAAGAACGAAAAGACTG
GATCAAATTTTTGTGAGGCCAAATCCAGATACAAAACGTGTACCTAGTACTGTCTTTATC
CATGAAAATGGTATCAGATTTCAATCCCCACTAAGGACTGATAGCAGAATAGACATATTA
TTCTCAAACATTAAGAATCTAATTTTTCAATCTTGTAAAGGTGAATTAATTGTCGTCATT
CATATTCATTTGAAGAATCCAATTTTAATGGGTAAAAAGAAAATACAAGACGTCCAATTC
TATCGTGAAGCTTCTGATATGTCCGTCGATGAAACTGGAGGTGGAAGACGAGGTCAATCC
AGATTCAGAAGATATGGTGATGAGGATGAGCTGGAACAAGAACAAGAAGAAAGAAGAAAA
CGAGCTGCGCTTGATAAAGAATTTAAGTATTTTGCAGACGCAATCGCAGAAGCATCAAAC
GGTTTATTGACGGTGGAGAATACATTTAGAGATTTGGGCTTCCAAGGTGTCCCAAATAGA
TCGGCAGTTTTCTGTATGCCAACTACAGATTGTTTAGTTCAATTGATTGAACCACCATTT
TTGGTGATTAACCTAGAGGAAGTCGAAATCTGTATTTTAGAAAGAGTTCAATTTGGTTTG
AAAAACTTCGACATGGTTTTTGTTTATAAAGATTTCAATAAACCCGTCACTCATATCAAT
ACAGTTCCGATCGAATCTTTAGATTTCTTGAAGCAGTGGTTAACAGATATGGATATTCCT
TACACTGTCTCAACAATCAATTTGAATTGGGCAACTATTATGAAGTCATTACAAGATGAT
CCATATCAGTTTTTCTTAGATGGTGGTTGGAATTTTCTGGCTACTGGTTCAGATGATGAA
GCATCTGATGAAAGTGAAGAAGAAGCTAGTGAATATGAGGCTTCAGAAGACGACGTAAGT
GATGAAAGCGCATTTTCTGAAGATGAAGAAGGATCAGAGGTGGATGACGATATTAGTGGT
GATGAAAGTGAGGACTATACTGGCGACGAGAGTGAGGAAGGTGAAGACTGGGATGAATTA
GAGAAAAAGGCTGCTAGGGCTGATAGGGGTGCAAACTTTAGAGATTAG
Protein sequence predicted based on nucleotide sequence
MEELNIDFDVFKKRIELLYSKYNEFEGSPNSLLFVLGSSNAENPYQKTTILHNWLLSYEF PATLIALVPGKVIIITSSAKAKHLQKAIDLFKDPESKITLELWQRNNKEPELNKKLFDDV IALINSAGKTVGIPEKDSYQGKFMTEWNPVWEAAVKENEFNVIDISLGLSKVWEVKDVNE QAFLSVSSKGSDKFMDLLSNEMVRAVDEELKITNAKLSDKIENKIDDVKFLKQLSPDLSA LCPPNYKFNFDLLDWTYSPIIQSGKKFDLRVSARSTNDQLYGNGCILASCGIRYNNYCSN ITRTFLIDPSEEMANNYDFLLTLQKEIVTNILKPGRTPKEVYESVIEYIEKTKPELVPNF TKNIGSLIGLEFRDSNFILNVKNDYRKIQRGDCFNISFGFNNLKDSQSANNYALQLADTV QIPLDETEPPRFLTNYTKAKSQISFYFNNEEEDNNKKKSSPATKVPSKPDRNSKILRTKL RGEARGGAEDAQKEQIRKENQKKLHEKLEKNGLLRFSAADANGPDSEPRQYFKKYESYVR DSQLPTNIRDLRIHVDWKSQTIILPIYGRPVPFHINSYKNGSKNEEGEYTYLRLNFNSPG SSGGISKKVEELPYEESADNQFIRSITLRSKDGDRMSETFKQIADLKKEATKREQERKAL ADVVQQDKLIENKTGRTKRLDQIFVRPNPDTKRVPSTVFIHENGIRFQSPLRTDSRIDIL FSNIKNLIFQSCKGELIVVIHIHLKNPILMGKKKIQDVQFYREASDMSVDETGGGRRGQS RFRRYGDEDELEQEQEERRKRAALDKEFKYFADAIAEASNGLLTVENTFRDLGFQGVPNR SAVFCMPTTDCLVQLIEPPFLVINLEEVEICILERVQFGLKNFDMVFVYKDFNKPVTHIN TVPIESLDFLKQWLTDMDIPYTVSTINLNWATIMKSLQDDPYQFFLDGGWNFLATGSDDE ASDESEEEASEYEASEDDVSDESAFSEDEEGSEVDDDISGDESEDYTGDESEEGEDWDEL EKKAARADRGANFRD*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3108
Mutations:
None
Discrepancy:
g1867a,V623I; t2906c,V969A
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
yeast
Marker:
LEU2
Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
ZM552
Synonyms:
ZM552
Description:
Yeast expression vector with N-terminal 3X V5 epitope tag; ampicillin resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
Comments:
ZM511 Stu1 vector plus Gateway attR frame B cassette EcoRV fragment; same as ZM515. Cloning into the vector removes the ccdB cassette and chloramphenicol resistance.
Size (bp):
8302
Parent Vector:
None
Properties:
Gateway, acceptor (destination), recombinational cloning, with tag/fusion/marker, yeast expression
Author Name:
Zarmik Moqtaderi
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
primer site
M13F
M13 forward sequencing primer
8286
8301
primer site
M13R
M13 reverse sequencing primer
2630
2647
promoter
CMD1
CMD1 promoter
295
484
recombination site
attR1
attR1 recombinational cloning site
650
666
recombination site
attR2
attR2 recombination site
2337
2352
reporter gene
LacZ
LacZ alpha gene
8146
8214
selectable marker
AmpR
ampcillin resistance
3795
4454
selectable marker
CmR
chloramphenicol resistance. removed with the ccdB cassette in clones with insert
883
1539
selectable marker
LEU2
LEU2 selectable marker in yeast
5273
5344
tag
V5
N-terminal triple V5 tag
494
631
trxn termination sequence
CYC1
CYC1 terminator
2377
2582
yeast ARS
ARS
ARS yeast origin of replication
0
0
yeast centromere
CEN
yeast centromere
0
0
