Vector Name:
pSG5
Synonyms:
pSG-5
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Mammalian expression vector with SV40 promoter and origin, beta-blobin intron, T7; amp resistance; restriction enzyme cloning.
Comments:
Highest expression of insert in T-antigen expressing cells
Size (bp):
4100
Parent Vector:
None
Properties:
in vitro transcription, mammalian expression, multiple cloning site, ssDNA production
Author Name:
Stratagene
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (EcoRI BamHI BglII)
1043
1055
bacterial origin
pUC ori
pUC-type ColE1 origin
1342
2009
intron
beta-globin intron
beta-globin intron
395
967
poly-A signal
SV40 polyA
SV40 PolyA signal sequence
1069
1202
promoter
SV40
SV40 promoter and origin
28
366
promoter
T7
T7 prokaryotic promoter
1022
1040
selectable marker
AmpR
ampicillin resistance gene (bla)
2160
3017
ssDNA origin
f1 ori
f1 origin for ssDNA production
3587
3893
viral origin
SV40 ori
SV40 origin
28
366
