Detailed Vector Information: pCIG
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Mammalian expression vector with CMV promoter, nuclear EGFP fluorescent marker, IRES, intron; amp resistance; restriction enzyme cloning.
Comments:
Made by 3xnls linker added to Clontech pIRES2-EFP then that into pCAGGS (Niwa et al. 1991)
Properties:
fluorescent marker, mammalian expression, multiple cloning site, with tag/fusion/marker
Author Name:
Sean Megason
Andrew McMahon
Publications:
Title: A mitogen gradient of dorsal midline Wnts organizes growth in the CNS
Type
Name
Description
Start
End
IRES
IRES
internal ribosome entry site
0
0
MCS
MCS
multiple coding sequence
0
0
poly-A signal
polyA
polyA site
0
0
promoter
CMV
CMV promoter
0
0
promoter
beta-actin
beta-actin promoter
0
0
reporter gene
nucEGFP
3x nuclear localization signal EGFP
0
0