Vector Name:
pHAGE-CMV-fullEF1a-IRES-ZsGreen
Synonyms:
pHAGE-CMV-fullEF1a-IRES-ZsGreen-W, pHAGE-CMV-EF1a-IRES-ZsGreen, pHAGE-CMV-fullEF1a-IRES-ZsGreen-W
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Lentiviral vector with internal EF1a promoter, has IRES-ZsGreen (IRES-fluorescent tag); amp resistance; restriction enzyme cloning.
Comments:
ZsGreen reported to be like GFP but brighter (statement not evaluated at the DNA Resource Core).
Size (bp):
8346
Parent Vector:
None
Properties:
fluorescent marker, mammalian expression, mammalian transduction, multiple cloning site, retroviral vector, with tag/fusion/marker
Author Name:
Jeng-Shin Lee
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
IRES
IRES
internal ribosome entry site (IRES)
0
0
MCS
MCS
MCS (between fEF1 promoter and IRES is NotI, NheI, XbaI, BamHI)
0
0
bacterial operon
LacO
LacO fragment
0
0
bacterial origin
ColE1 ori
ColE1-type origin of replication
0
0
fluorescent marker
ZsGreen
ZsGreen fluorescent coding sequence (reportedly like GFP but brighter)
0
0
intervening sequence
cpPu
central polypurine track
0
0
intervening sequence
3'Flank
3' flanking fragment
0
0
promoter
EF-1 alpha pr
EF1a promoter (full-length)
0
0
selectable marker
AmpR
ampicillin resistance gene
0
0
viral LTR
LTR
viral long terminal repeat (LTR)
0
0
viral LTR
dU3LTR
viral long terminal repeat (LTR)
0
0
viral origin
SV40 ori
SV40 origin (and promoter)
0
0
viral response element
RRE
rev response element
0
0
viral response element
WPRE
WPRE response element
0
0
