Vector Name:
pET22b(+)
Synonyms:
pET22, pET22b, pET22bplus, pET22b+
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Expression vector with T7 promoter and terminator flanking MCS that accepts insert, has pelB leader sequence for subcellular targeting and tag cds (may be lost with some insertion strategies); amp resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
5493
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ssDNA production, with tag/fusion/marker
Author Name:
Novagen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (AvaI, XhoI, NotI, EagI, HindIII, SalI, SacI, EcoRI, BamHI, NcoI)
158
225
bacterial origin
E. coli ori
pBR322 origin
3277
0
periplasmic localization signal
pelB
pelB coding sequence (signal sequence for periplasmid localization)
224
289
promoter
T7
T7 promoter
361
377
repressor protein gene
lacI
lacI coding sequence
764
1843
selectable marker
AmpR
ampicillin resistance gene
4038
4895
ssDNA origin
f1 ori
f1 origin for ssDNA production
5027
5482
tag
His
His tag
140
157
