Detailed Vector Information: pTrc99A
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Bacterial expression vector with inducible lacI promoter; amp resistance; restriction enzyme cloning.
Comments:
IPTG induction of protein expression (1mM IPTG)
Properties:
bacterial expression, inducible expression, multiple cloning site
Author Name:
KJ Abel
E Amann
Publications:
Title: Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
0
0
promoter
lac pr
lac promoter (induce with 1mM IPTG)
0
0
selectable marker
AmpR
ampicillin resistance gene
0
0