Detailed Vector Information: pTrc99A

Vector Name:
pTrc99A
Synonyms:
pTrc99
Sequencing Primer:
Forward:  Unknown
Reverse:  Unknown
Description:
Bacterial expression vector with inducible lacI promoter; amp resistance; restriction enzyme cloning.
Comments:
IPTG induction of protein expression (1mM IPTG)
Size (bp):
4176
Parent Vector:
None
Properties:
bacterial expression, inducible expression, multiple cloning site
Author Name:
KJ Abel
E Amann
Publications:
PMID: 3069586
Title: Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
0
0
promoter
lac pr
lac promoter (induce with 1mM IPTG)
0
0
selectable marker
AmpR
ampicillin resistance gene
0
0