Vector Name:
p283
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector, adds His tag, C-terminal TEV cleavage site fusion; kanamycin resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
5282
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
PSI
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
1421
2103
promoter
T7
T7 promoter
4984
5003
repressor binding site
lacO
lacO regulatory sequence
5003
5025
repressor protein gene
lacI
lacI coding region
3518
4597
selectable marker
KanR
kanamycin resistance gene
1375
563
ssDNA origin
f1 ori
f1 origin
17
457
trxn termination sequence
T7 term
T7 termination sequence
5211
5257
