Detailed Vector Information: pET28-N-His-thromb

Vector Name:
pET28-N-His-thromb
Synonyms:
N-His-Thrombin pET28
Sequencing Primer:
Forward:  T7
Reverse:  ISFI_newrev
Description:
Expression vector with T7 promoter, adds N-terminal His tag, thrombin site; kanamycin resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
5369
Parent Vector:
None
Properties:
cell-free expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name:
Integrated Center for Structure and Function Innovation
Publications:
PMID: 16374842
Title: Crystal structure of a putative pyridoxine 5'-phosphate oxidase (Rv2607) from Mycobacterium tuberculosis
PMID: 16885468
Title: Solution structure of the conserved hypothetical protein Rv2302 from Mycobacterium tuberculosis
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
1421
2103
primer
reverse
reverse sequencing primer 5?-TTTGTTAGCAGCCGGATCTCAGTGG-3?
5861
5885
promoter
T7
T7 promoter
4987
5006
protease cleavage site
Thrombin
N-terminal His/thrombin cleavage site
0
0
repressor protein gene
LacI
LacI
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
N-terminal 6xHis tag
5847
5864
trxn regulatory element
lac operator
lac operator
5006
5028