Detailed Vector Information: pEU-His-FV

Vector Name:
pEU-His-FV
Synonyms:
''
Sequencing Primer:
Forward:  pEU319 Forward
Reverse:  pEU Reverse
Description:
Cell-free expression vector, adds N-terminal 6xHis tag; amp resistance; restriction enzyme cloning (SgfI, PmeI).
Comments:
Cloning into this vector will remove the BarCat casstte. To avoid, the toxicity of the barnase gene, the vector pEU-HSBC (EvNO00306667) can be ordered, which substitutes sacB for barnase.
Size (bp):
4966
Parent Vector:
None
Properties:
cell-free expression, multiple cloning site, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
Publications:
PMID: 15782178
Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
2809
3491
gene fragment
Omega
TMV Omega
397
520
gene fragment
TMV-L
TMV-L
278
2238
gene fragment
BarCat-bar
BarCat cassette, including Barnase
572
908
gene fragment
BarCat-cat
BarCat cassette, including CAT
1010
1667
primer
His2 forward
His2 forward sequencing primer TCTTTCAAATACTTCTAGCTAGAGTA
424
449
promoter
SP6
SP6 promoter
373
390
recombination site
MCS
pF1K (Flexi-Vector) homology domain
1675
1803
selectable marker
AmpR
ampicillin resistance gene
3589
4248
tag
His
6xHis tag
527
544