Detailed Vector Information: pSGX2

Vector Name:
pSGX2
Synonyms:
pSGX-2, pSGX2_BN
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds N-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5328
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
TOPO recognition site
TOPO
TOPO cloning site
5097
5106
bacterial origin
ColE
ColE1 bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4984
5003
repressor protein gene
LacI
LacI cds
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
N-terminal 6xHis tag
5078
5095
trxn regulatory element
lac operator
lac operator
5003
5025