Detailed Vector Information: pMCSG19C

Vector Name:
pMCSG19C
Synonyms:
''
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC).
Comments:
as 19 + constitutive expression of TVMV protease from integrated gene
Size (bp):
7468
Parent Vector:
None
Properties:
in vitro transcription, ligation independent cloning (LIC)
Author Name:
Midwest Center for Structural Genomics
M.Donnelly
Midwest Center for Structural Genomics
PSI
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
5432
5432
gene fragment
TVMV cds
TVMV protease gene
1927
2637
ligation independent cloning
LIC
LIC Site
204
235
promoter
T7
T7 promoter
1515
1531
protease cleavage site
TEV
protease cleavage site
223
243
protease cleavage site
TVMV
TVMV protease cleavage site
286
306
repressor binding site
lac operator
LacO fragment
1490
1512
repressor binding site
tet operator
TetO fragment
1753
1908
repressor protein gene
LacI
LacI CDS
3072
4031
selectable marker
AmpR
ampicillin resistance gene
6220
7080
tag
His
N-terminal 6x His tag
268
285
tag
MBP
N-terminal MBP tag
337
1443
trxn termination sequence
T7 term
T7 terminator
26
72