Vector Name:
pVP65K
Synonyms:
''
Sequencing Primer:
Forward:
MBP Forward
Reverse:
Unknown
Description:
Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette.
Comments:
Vector contains a lethal BarCat cassette that requires the strain BR610 for growth. Lethal cassette is removed during cloning.
Size (bp):
7174
Parent Vector:
None
Properties:
bacterial expression
Author Name:
University of Wisconsin
Center for Eukaryotic Structural Genomics
PSI
Center for Eukaryotic Structural Genomics
PSI
Publications:
PMID:
18988018
Title: Flexi vector cloning.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 bacterial origin of replication
4977
6204
gene fragment
TVMV cds
TVMV gene
139
849
gene fragment
BarCat
BarCat cassette including Barnase
2245
3376
primer
MBP forward
MBP forward primer
2071
2091
primer
T7 term
T7 terminator reverse primer
3426
3443
promoter
T5
T5 promoter region
952
1032
promoter
TET
TET promoter
873
946
promoter
LacIq
LacIq-wt promoter
4899
4967
protease cleavage site
TVMV
TVMV protease cleavage site
2189
2209
repressor binding site
LacI
LacI coding region
3807
4898
selectable marker
KanR
Kanamycin resistance gene
6213
7174
tag
MBP
N-terminal maltose binding protein tag
1061
2165
tag
His
8xHis tag
2210
2234
trxn termination sequence
rrnB
rrnB transcription terminator
15
111
