Vector Name:
pSGP47
Synonyms:
''
Sequencing Primer:
Forward:
pSGP-Forward
Reverse:
pSGP-Reverse
Description:
Yeast expression vector with Adh2 promoter, adds 3C protease cleavage site and 10xHis tag; ampicillin resistance (bacteria); uracil (yeast); ligation independent cloning (LIC).
Comments:
S. cerevisiae expression strain
Size (bp):
6296
Parent Vector:
None
Properties:
ligation independent cloning (LIC), with tag/fusion/marker, yeast expression
Author Name:
Mark Dumont
Nadia Fedoriw
Center for High Throughput Structural Biology
PSI
Nadia Fedoriw
Center for High Throughput Structural Biology
PSI
Publications:
PMID:
20045057
Title: Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ligation independent cloning
LIC
Ligation independent cloning site
4220
4231
primer
primer
sequencing primer: CAATCAACTATCAACTATTAACTA
4155
4178
promoter
Ura3 promoter
Ura3 promoter
5981
6010
protease cleavage site
3C
Rhinovirus 3C protease cleavage site
4233
4256
selectable marker
AmpR
ampicillan selectable marker
1742
2401
selectable marker
Ura3
URA3 nutritional marker
5092
5895
tag
His
10xHis tag
4275
4304
