Detailed Vector Information: pMCSG26
Description:
Bacterial expression vector with C-terminal 6xHis tag and T7 promoter; ampicillin resistance in bacterial; ligation independent cloning (LIC)
Comments:
For more information about MCSG vectors visit their website at: http://bioinformatics.anl.gov/mcsg/technologies/vectors.html
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
Publications:
Title: Cleavable C-terminal His-tag vectors for structure determination.
Title: Structural and functional analysis of AsbF: origin of the stealth 3,4-dihydroxybenzoic acid subunit for petrobactin biosynthesis
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin of replication
3220
3220
primer
T7 forward
T7 forward sequencing primer
299
315
primer
T7 reverse
T7 terminator reverse sequencing primer
66
84
promoter
T7
T7 promoter
300
316
recombination site
LIC
LIC site
217
238
repressor binding site
lac operator
LacO fragment
275
297
repressor protein gene
LacI
Lac I cds
830
1789
selectable marker
AmpR
Ampicillin resistance gene
3978
4838
tag
His
C-terminal 6xHis tag
208
225
trxn termination sequence
T7 term
T7 terminator
26
72