Detailed Vector Information: pENTR201
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.
Comments:
derived from pDONR201, available from Invitrogen Corp. More information can be found here http://orfeomecollaboration.org/vectors.php
Properties:
Gateway, donor (entry), recombinational cloning
Type
Name
Description
Start
End
bacterial origin
pUC ori
pUC bacterial origin of replication
1629
2268
primer
pDONR201-forward
pDONR201-forward sequencing primer GTAACATCAGAGATTTTGAGACAC
570
593
primer
pDONR201-reverse
pDONR201-reverse sequencing primer TCGCGTTAACGCTAGCATGGATCTC
307
331
recombination site
attL1
attL recombination site 1 present in recombined (with insert) form only
418
338
recombination site
attL2
attL recombination site 2 present in recombined (with insert) form only
488
576
selectable marker
KanR
Kanamycin resistance gene
699
1508
trxn termination sequence
rrnB
T1-T2 rrnb RNA termination
322
56