Vector Name:
pCAG.OSF
Synonyms:
''
Description:
Mammalian expression vector with a CBA promoter and N-terminal Flag tag; ampicillin resistance in bacteria; restriction enzyme cloning (Cla1 and Kpn1)
Comments:
Empty vector Sundquist Lab internal database number = WISP 06-65. Myc tag sequence of pCAG.Myc was substituted with One-STrEP-FLAG tag sequence to create this vector. Restriction enzyme cloning can be used with this vector - e.g. Cla1, Kpn1 works well
Size (bp):
4980
Parent Vector:
None
Properties:
mammalian expression, multiple cloning site, with tag/fusion/marker
Author Name:
University of Utah
Center for Membrane Proteins in Infectious Diseases
Kathy Sykes
Brenda Hogue
Center for Membrane Proteins in Infectious Diseases
Kathy Sykes
Brenda Hogue
Publications:
PMID:
19525971
Title: Structural basis for ESCRT-III protein autoinhibition.
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin
0
0
primer
CAGF
forward sequencing primer (5' TCGGCTTCTGGCGTGTGACC 3'
)
1606
1625
primer
CAGR
reverse sequencing primer (5' TGTCCCCATAATTTTTGGCAGAGG
3')
2010
2033
promoter
CBA
Chicken beta-actin (CBA) promoter
0
0
protease cleavage site
Enterokinase
enterokinase (EK) cleavage recognition sequence
0
0
selectable marker
AmpR
ampicillin resistance marker
3992
4651
tag
Flag
N-terminal Flag tag
1858
1881
