Vector Name:
pGEX2T(TEV)
Synonyms:
''
Description:
Bacterial Expression vector with an N-terminal GST tag and TEV site; ampicillin resistance in bacteria; restriction enzyme cloning.
Comments:
Sundquist Lab internal database number = WISP 01-69; Nde1, BamH1 restriction enzyme cloning can be used easily with this vector
Size (bp):
4993
Parent Vector:
None
Properties:
bacterial expression, with tag/fusion/marker
Author Name:
University of Utah
Wesley I. Sundquist
Wesley I. Sundquist
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
primer
forward primer
pGEX forward sequencing primer GGTTCCGCGTGGATCTCCGG
920
939
primer
reverse primer
pGEX reverse sequencing primer CCGGGAGCTGCATGTGTCAGAGG
1043
1065
promoter
tac promoter
tac promoter
180
209
protease cleavage site
TEV
N-terminal TEV protease cleavage site
0
0
reporter gene
LacZ
LacZ alpha
4655
0
repressor binding site
lac operator
lac operator binding site
215
238
selectable marker
AmpR
Ampicillin resistance
1599
2259
tag
GST
N-terminal GST tag
258
929
