Vector Name:
pSpeedE5T
Synonyms:
''
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial expression vector with a pBAD/T7 promoter and TEV-cleavable N-terminal 6xHis tag; arabinose-inducible; kanamycin resistance in bacteria; PIPE cloning
Comments:
None
Size (bp):
4058
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
PSI:Biology
PSI:Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
death cassette
ccdB
ccdB death cassette (not present in plasmids containing gene inserts)
403
1808
primer
pBADfwd
pBAD forward sequencing primer
208
227
primer
pBADrev
pBAD reverse sequencing primer
1909
1926
promoter
T7
T7 promoter
291
307
promoter
pBAD
pBAD promoter
250
277
protease cleavage site
TEV
TEV protease cleavage site
382
402
repressor protein gene
araC
AraC ORF
3157
4032
selectable marker
KanR
kanamycin resistance
2396
3211
tag
His
N-terminal 6xHis tag MGSDKIHHHHHENLYFQG
346
402
trxn regulatory element
arabinose O1 operator
arabinose O1 operator region
4
19
trxn regulatory element
arabinose O2 operator
arabinose O2 operator region
161
182
