Vector Name:
pet16B-PP
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Bacterial expression vector with T7 promoter and N-terminal 10X His tag and precision protease cleavage site; ampicillin resistance in bacteria; restriction enzyme cloning
Comments:
None
Size (bp):
5717
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Wesley I. Sundquist
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
1164
1846
primer
T7 forward
T7 forward sequencing primer TAA TAC GAC TCA CTA TAG G
5230
5248
primer
T7 reverse
T7 terminator sequencing primer GCT AGT TAT TGC TCA GCG G
5444
5462
promoter
T7
T7 promoter
5230
5249
repressor binding site
lac operator
lac operator
5249
5271
repressor protein gene
LacI
LacI
3761
4840
selectable marker
AmpR
ampicillin resistance
407
1066
tag
His
N-terminal 10X His tag
5323
5352
trxn termination sequence
T7 term
T7 terminator
5459
5505
