Vector Name:
pMinVecK
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Bacterial expression vector used in the pGTDelta238 plasmid with a T5 promoter; kanamycin resistance in bacteria; restriction enzyme cloning
Comments:
This vector only exists in silico
Size (bp):
3914
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site
Author Name:
University of Wisconsin
Brian Fox
Paul Blommel
Center for Eukaryotic Structural Genomics
Brian Fox
Paul Blommel
Center for Eukaryotic Structural Genomics
Publications:
PMID:
17543538
Title: A combined approach to improving large-scale production of tobacco etch virus protease.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin
1711
2938
primer
forward primer
"Upstream NcoI" forward sequencing primer 5' TTGCTTTGTGAGCGGATAAC 3'
141
160
primer
reverse primer
"MinVec Rev" reverse sequencing primer 5' AAGCTTCGAACGCGTATCG 3'
310
329
promoter
T5
T5 promoter
122
202
repressor protein gene
LacI
LacI-wt coding region
541
1632
selectable marker
KanR
Kanamycin resistance gene
2946
3740
trxn termination sequence
rrnB
rrnB terminator
357
453
