Vector Name:
pET151-PP
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Bacterial expression vector with a T7 promoter and a prescission protease-cleavable N-terminal 6xHis V5 tag; ampicillin resistance in bacteria; sequence and ligation independent (SLIC) cloning
Comments:
None
Size (bp):
5754
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
University of Utah
Christopher P. Hill
Han Han
Christopher P. Hill
Han Han
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
1897
2579
copy control gene
ROP
Repressor of protein (ROP)
3003
3179
promoter
T7
T7 promoter
209
228
protease cleavage site
3C
3C prescission protease cleavage site
359
383
repressor binding site
lac operator
Lac operator sequence
228
250
repressor protein gene
LacI
LacI
4494
5573
selectable marker
AmpR
Ampicillin resistance gene
1140
1799
tag
His
N-terminal 6xHis tag
300
317
tag
V5
N-terminal V5 epitope tag
318
359
trxn termination sequence
T7 term
T7 terminator
467
513
