Vector Name:
pLenti4/V5-DEST
Synonyms:
''
Sequencing Primer:
Forward:
CMV forward
Reverse:
V5 reverse
Description:
Mammalian lentiviral expression vector with a CMV promoter and C-terminal V5 tag; ampicillin and zeocin resistance in bacteria; zeocin resistance in mammalian cells; Gateway recombinational cloning
Comments:
None
Size (bp):
8634
Parent Vector:
None
Properties:
Gateway, acceptor (destination), mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pUC ori
pUC bacterial origin
7554
8227
death cassette
ccdB
ccdB death cassette (removed in plasmids containing an insert)
3674
3979
packaging signal
HIV-1 psi
HIV-1 psi packaging signal
521
565
promoter
RSV
RSV/5;LTR hybrid promoter
1
410
promoter
CMV
CMV promoter
1809
2392
promoter
EM7
EM7 promoter
4621
4687
promoter
SV40
SV40 promoter
4293
4602
recombination site
attR1
attR1 recombination site
2440
2564
recombination site
attR2
attR2 recombinational cloning site
4020
4144
response element
HIV-1 RRE
HIV Rev Response Element (RRE)
1075
1308
selectable marker
CmR
chloramphenicol resistance gene (removed in plasmids containing an insert)
2673
3332
selectable marker
ZeoR
Zeocin resistance gene
4688
5062
selectable marker
AmpR
ampicillin resistance gene
6549
7409
splice acceptor site
3' splice acceptor
3' splice acceptor
1656
1656
splice donor site
5' splice donor
5' splice donor
520
520
tag
V5
C-terminal V5 epitope tag
4197
4238
