Vector Name:
pJFT7_nFlag_cHalo_DC
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal Flag and C-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
None
Size (bp):
6238
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Justin Saul
Ji Qiu
Fernanda Festa
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3523
4205
death cassette
ccdB (BsrGI removed)
ccdB death cassette (removed in plasmids containing an insert)
1798
2103
enhancer
CITE
CITE enhancer
17
515
primer
forward primer
nap150f forward sequencing primer cccattgtatgggatctgatc
388
408
primer
reverse primer
cHaloseqR reverse sequencing primer CAACATCGACGTAGTGCAT
2384
2402
promoter
T7
T7 promoter
6214
3
protease cleavage site
TEV
TEV protease cleavage site
2291
2311
recombination site
attR1
attR1 recombinational cloning site
562
588
recombination site
attR2
attR2 recombinational cloning site
2244
2268
reporter gene
LacZ
LAcZ alpha
3059
3127
selectable marker
AmpR
Ampicillin resistance
4303
4962
selectable marker
CmR
chloramphenicol resistance (removed in plasmids containing an insert)
797
1453
ssDNA origin
f1
f1 origin
5731
6037
tag
HALO
C-terminal Halo tag
2327
3211
tag
Flag
N-terminal Flag tag
531
554
trxn termination sequence
T7 term
T7 transcriptional termination
3294
3341
