Vector Name:
pGBKT7
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Yeast two-hybrid vector with a T7 and ADH1 promoter and N-terminal c-myc tag; TRP1 resistance in yeast, kanamycin resistance in bacteria; restriction enzyme cloning
Comments:
pGADT7 AD is a yeast two-hybrid expression vector that is designed to express a fusion protein between the GAL4 activation domain (AD; amino acids 768–881) and a protein of interest.
Size (bp):
7303
Parent Vector:
None
Properties:
multiple cloning site, two-hybrid, with tag/fusion/marker, yeast expression
Author Name:
Clonetech
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
Multiple cloning site
1281
1334
bacterial origin
pUC ori
pUC origin
1838
2636
binding site
Gal4 Act
Gal4 DNA binding domain
762
1202
promoter
ADH1
truncated ADH1 promoter
30
736
promoter
T7
T7 promoter
1212
1235
selectable marker
KanR
Kanamycin resistance
3221
4143
selectable marker
TRP1
TRP1 selectable marker in yeast
6030
6704
tag
c-myc
N-terminal c-myc tag
1248
1280
trxn termination sequence
T7 term
T7 terminator
1335
1381
trxn termination sequence
ADH1 term
ADH1 transcriptional terminator
1414
1610
