Vector Name:
pGEX2T(PP)
Synonyms:
WISP 08-16
Description:
Bacterial expression vector with tac promoter and N-terminal GST tag and PP site; ampicillin resistance in bacteria; Nde1, BamH1 useful in addition to SLIC cloning
Comments:
Empty vector Sundquist Lab internal database number = WISP 08-16. Note: Usually use pGEX-PP-GFP for SLIC cloning and negative selection (WISP10-32)
Size (bp):
4996
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
University of Utah
Wesley I. Sundquist
Wesley I. Sundquist
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
2359
3041
primer
forward primer
pGEX5p forward sequencing primer GGGCTGGCAAGCCACGTTTGGTG
869
891
primer site
reverse primer
pGEX Reverse sequencing primer CCGGGAGCTGCATGTGTCAGAGG
1046
1068
promoter
tac promoter
tac promoter
180
208
protease cleavage site
PP
PP site
939
962
repressor binding site
lac operator
lac operator
215
237
repressor protein gene
LacI
LacI
3348
4427
selectable marker
AmpR
ampicillin resistance
1602
2261
tag
GST
N-terminal GST tag
258
929
