Vector Name:
pET11HisPP
Synonyms:
WISP11-262
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter and N-terminal precision protease-cleavable 7xHis tag; ampicillin resistance in bacteria; NheI, XhoI restriction enzyme cloning
Comments:
Empty vector Sundquist Lab internal database number = WISP11-262
Size (bp):
5728
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
University of Utah
Wesley I. Sundquist
Devin Christensen
Wesley I. Sundquist
Devin Christensen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
1164
1846
promoter
T7
T7 promoter
5230
5249
protease cleavage site
PP
Precision protease cleavage site
5348
5371
repressor binding site
lac operator
lac operator
5249
5271
repressor protein gene
LacI
LacI
3761
4840
selectable marker
AmpR
ampicillin resistance
407
1066
tag
His
N-terminal 7xHis tag
5321
5341
trxn termination sequence
T7 term
T7 terminator
5470
5516
