Vector Name:
pGEX-PP-GFP
Synonyms:
WISP 10-32
Description:
Bacterial expression vector with tac promoter and N-termianl GST pag and precision protease cleavage site; GFP insert is removed in clones with inserts; ampicillin resistance in bacteria; ligation independent cloning
Comments:
This construct is used as a cloning intermediate
Final constructs will not contain the GFP insert.
Sundquist Lab internal database number = WISP 10-32
Size (bp):
5704
Parent Vector:
None
Properties:
bacterial expression, fluorescent marker, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
University of Utah
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3067
3749
gene fragment
GFP
GFP insert. GFP is replace by your insert of interest.
972
1382
promoter
tac promoter
tac promoter
180
208
protease cleavage site
PP
precision protease cleavage site
939
962
repressor binding site
lac operator
lac operator
215
237
repressor protein gene
LacI
LacI
4056
5135
selectable marker
AmpR
ampicillin resistance
3210
3969
tag
GST
N-terminal GST
258
929
